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Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina

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Abstract

Fusarium graminearum is the primary causal agent of Fusarium head blight of wheat in Argentina. This disease affects yield losses and quality of grains, reducing the wheat end-use, also causing mycotoxin contamination. In this study, the genetic variability and deoxynivalenol (DON) potential/production of F. graminearum sensu stricto (s.s.) isolates obtained from wheat samples of the 2009, 2010, and 2011 growing seasons from a single location in Argentina were evaluated. The genetic variability detected using inter-simple sequence repeat (ISSR) was analyzed in relation to the in vitro deoxynivalenol production, the main monitored and quantified mycotoxin according to the current regulations for the international marketing of cereals. Of the 68 F. graminearum s.s. isolates obtained in this study, 95 % showed a different banding pattern with ISSR molecular markers and a high variability was detected within the population. However, no clustering was found in relation with year or DON production. All isolates amplify for the DON-related gene and a high variability in DON production was observed among the isolates, with production values between non-producers and 1741 μg/g. The results suggest that the F. graminearum s.s. population varies significantly in both genetic structure and toxin production in a limited sampled area.

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Acknowledgments

We thank Consejo Nacional de Investigaciones Científicas y Tecnológicas (grants PIP 0925 and PIP 0551) and FONCYT PICT PICT-2011-0851 and 030/2011 for financial support, and the technical assistance of Esteban González.

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Authors and Affiliations

  1. Facultad de Ciencias Exactas, Centro de Investigación y Desarrollo en Fermentaciones Industriales (CINDEFI), Universidad Nacional de La Plata (UNLP); CCT-La Plata, CONICET, Calle 47 y 115, B1900ASH, La Plata, Argentina

    Leonel M. Ortega, Andrea L. Astoreca & Teresa M. Alconada

  2. Laboratorio de Biología Funcional y Biotecnología (BIOLAB)-CICBA-INBIOTEC-CONICET, Facultad de Agronomía de Azul, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Av. República de Italia N° 780, 7300, Azul, Buenos Aires, Argentina

    María I. Dinolfo & Sebastián A. Stenglein

  3. Cátedra de Microbiología, Facultad de Agronomía de Azul, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA), Av. República de Italia N° 780, 7300, Azul, Buenos Aires, Argentina

    Sebastián A. Stenglein

  4. Patología Vegetal del Cultivo de Trigo, Instituto Nacional de Tecnología Agropecuaria (INTA), Ruta 12, km 3, 2580, Marcos Juárez, Córdoba, Argentina

    Enrique J. Alberione

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  1. Leonel M. Ortega
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  2. María I. Dinolfo
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Ortega, L.M., Dinolfo, M.I., Astoreca, A.L. et al. Molecular and mycotoxin characterization of Fusarium graminearum isolates obtained from wheat at a single field in Argentina. Mycol Progress 15, 1 (2016). https://doi.org/10.1007/s11557-015-1147-7

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