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Genetic diversity of Chinese Wolfiporia cocos cultivars revealed by phenotypic traits and ISSR markers

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Abstract

Wolfiporia cocos, a well-known medicinal fungus, has been widely used in China and other Asian countries due to its various therapeutic value. In this study, genetic diversity among 22 Chinese W. cocos cultivars were assessed using phenotypic traits and inter-simple sequence repeat (ISSR). The results demonstrated that a total of 686 bands were amplified by 17 selected primers, 81.9 % of which were found to be polymorphic. Genetic distance was from 1.05 to 7.62 (physiological characters), and the similarity coefficients were from 0.63 to 0.82 (ISSR). Both methods were effective in differentiating among strains. The unweighted pair-group method with arithmetic averages (UPGMA) dendrogram classified the 22 strains into six major groups. Comprehensive analysis indicated that the cultivated germplasm of W. cocos in China were rich in genetic diversity. The results of this study has contributed substantially to our knowledge of W. cocos genetic diversity and also provides a theoretical basis of use and conservation for W. cocos resource and breeding.

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Acknowledgments

We thank the anonymous reviewers for their constructive and helpful comments. This research was supported by the State of Traditional Chinese Medicine Industry Research Program (No. 201107009).

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    Authors and Affiliations

    1. College of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, 430023, People’s Republic of China

      Qiao Sun, Wenjun Zhu, Shaopeng Zhang, Jia Song, Yonglian Zheng & Ping Chen

    2. Institute for Interdisciplinary Research, Jianghan University, Wuhan, 430056, People’s Republic of China

      Wei Wei

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    1. Qiao Sun
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    2. Wei Wei
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    3. Wenjun Zhu
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    4. Shaopeng Zhang
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    Correspondence to Wenjun Zhu or Ping Chen.

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    Section Editor: Marc Stadler

    Qiao Sun and Wei Wei share joint first authorship.

    Qiao Sun and Wei Wei contributed equally to this work.

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    Sun, Q., Wei, W., Zhu, W. et al. Genetic diversity of Chinese Wolfiporia cocos cultivars revealed by phenotypic traits and ISSR markers. Mycol Progress 14, 61 (2015). https://doi.org/10.1007/s11557-015-1088-1

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