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Rhizoglomus melanum, a new arbuscular mycorrhizal fungal species associated with submerged plants in freshwater lake Avsjøen in Norway

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Abstract

A new species of arbuscular mycorrhizal (AM) fungi (Glomeromycota) was isolated from the rhizosphere of isoetid vegetation, namely of Littorella uniflora and Isoëtes lacustris, in the freshwater lake Avsjøen (Central Norway), and propagated on bait cultures on Zea mays and L. uniflora, respectively. The fungus is distinguished by chestnut brown to dark brown, triple-layered spores, (137–)165–226(−285) μm in diameter, and 1–2 concolorous subtending hyphae that regularly form multiple septae within 100–600 μm from the spore base, each approximately 10–25 μm apart, while the pore directly at the spore base is open. It can easily be differentiated from all other species in the Glomeraceae by the combination of spore size, spore color, and the characteristics of the subtending hyphae. Phylogenetic analyses of the partial SSU, ITS, and partial LSU nrDNA sequences placed the fungus in the genus Rhizoglomus, well separated from all other species in the genus. The fungus described here as Rhizoglomus melanum was hitherto only found in lake sediments which might suggest its specificity for aquatic environment. It is the first new AM fungus ever, isolated and propagated from submerged, aquatic vegetation.

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Acknowledgments

The authors thank Miroslava Opičková for her excellent technical assistance and Jan Suda for his help in the field. Hana Strusková is acknowledged for chemical analyses of the sediment. The study was supported by the Czech Science Foundation (project P504/10/0781) and by the Swiss National Science Foundation (project IZ73Z0_152740).

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Authors and Affiliations

  1. Institute of Botany, Academy of Sciences of the Czech Republic, CZ-242 43, Průhonice, Czech Republic

    Radka Sudová, Zuzana Sýkorová & Jana Rydlová

  2. Biology Centre, Institute of Hydrobiology, Academy of Sciences of the Czech Republic, CZ-370 05, České Budějovice, Czech Republic

    Martina Čtvrtlíková

  3. Federal Research Institute for Sustainability Sciences, Plant-Soil-Interactions, Agroscope, Reckenholzstrasse 191, CH-8046, Zürich, Switzerland

    Fritz Oehl

  4. Departamento de Micologia, CCB, Universidade Federal de Pernambuco, Av. da Engenharia s/n, Cidade Universitária, 50740-600, Recife, PE, Brazil

    Fritz Oehl

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  1. Radka Sudová
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Correspondence to Fritz Oehl.

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Sudová, R., Sýkorová, Z., Rydlová, J. et al. Rhizoglomus melanum, a new arbuscular mycorrhizal fungal species associated with submerged plants in freshwater lake Avsjøen in Norway. Mycol Progress 14, 9 (2015). https://doi.org/10.1007/s11557-015-1031-5

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