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Effect of oxidative stress on cell wall morphology in four pathogenic Candida species

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Abstract

Candida species are a primary cause of high mortality in immunocompromised and hospitalized patients. The cell wall (CW) of these pathogens is the first structure contacted by the reactive oxygen species (ROS) generated during respiratory bursting in phagocytic cells. The purpose of this work was to evaluate the effect of oxidative stress on CW and some metabolic activities of C. albicans, C. glabrata, C. krusei and C. parapsilosis. Data revealed that C. krusei is more resistant to H2O2, while C. albicans is more resistant to O2 •‾ ions. It was also observed that cells exposed to oxidative stress transited from the normal, oval morphology to other aberrant forms that exhibited indentations and protrusions. In addition, oxidative stress caused damage not only to the CW, but also altered a number of cell biological functions, such as lipidic peroxidation, antioxidant capacity, protein carbonylation and catalase activity. The morphological and other cellular changes undergone by Candida cells exposed to ROS in vitro are possibly similar to those occurring in vivo, and therefore may be important during infection and persistence of the pathogen in the human host.

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Acknowledgments

The authors thank the financial support granted to M. Cuéllar-Cruz by Proyecto-Institucional-UGTO-id202/2013 from Universidad de Guanajuato, México, and PROMEP-UGTO-PTC-328. The authors would like to thank John Dye for his invaluable support in reviewing the manuscript. We are also grateful to Prof. M.A. Martínez-Rivera (Departamento de Microbiología, ENCB-IPN, México), for kindly providing the clinical isolates of C. albicans, C. glabrata, C. krusei and C. parapsilosis used in this study. We are also grateful to Dr. Mario Ávila-Rodríguez and M. en C. Paulina Lozano-Sotomayor for the facilities provided and technical assistance in the SEM microphotographs. We also thank Dr. B. Franco, F. Padilla and C. Leal for providing some of the chemicals and equipment.

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The authors declare that there were no conflicts of interest with any organization or entity with a financial interest or financial conflict with the material discussed in this work.

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  1. Departamento de Biología, División de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta S/N, C.P., 36050, Guanajuato, Guanajuato, México

    Mayra Denisse Ramírez-Quijas, Roberto Zazueta-Sandoval, Armando Obregón-Herrera, Everardo López-Romero & Mayra Cuéllar-Cruz

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  1. Mayra Denisse Ramírez-Quijas
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  2. Roberto Zazueta-Sandoval
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Correspondence to Mayra Cuéllar-Cruz.

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Dedication: This contribution is dedicated to the late Julio César Cuéllar-Cruz. He will always be remembered as the beloved brother who always inspired me to pursuit my goals. He was also an example of perseverance to our family.

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Ramírez-Quijas, M.D., Zazueta-Sandoval, R., Obregón-Herrera, A. et al. Effect of oxidative stress on cell wall morphology in four pathogenic Candida species. Mycol Progress 14, 8 (2015). https://doi.org/10.1007/s11557-015-1028-0

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