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Mycological Progress

, Volume 13, Issue 1, pp 21–31 | Cite as

Boletus roseoflavus, a new species of Boletus in section Appendiculati from China

  • Haibo LiEmail author
  • Hailong Wei
  • Huazheng Peng
  • Hongmei Ding
  • Liling Wang
  • Liang He
  • Lizhong Fu
Original Article

Abstract

Boletus roseoflavus is described as a new species based on morphological and molecular studies of the type collection and additional materials. Boletus roseoflavus is morphologically distinct from other related species (B. regius, B. appendiculatus, and B. speciosus) by its light pink, pink, or rosy pileus, lemon yellow or golden yellow context, and lemon yellow or light yellow, enlarged download stipe with yellow reticulation. Phylogenetic analyses based on ribosomal DNA internal transcribed spacer regions and nuclear large subunit ribosomal RNA indicate that B. roseoflavus belongs to Boletus section Appendiculati.

Keywords

Morphology Taxonomy Molecular phylogeny Boletus sect. Appendiulati 

Notes

Acknowledgments

This work was supported by the Natural Science Foundation of Zhejiang Province, China (Grant Nos. Y3100345, Y3090380, Y3110048, and Y3110530) and the Project of Innovation team construction and Talents cultivation on Forest food research (Grant No. 2012F20012). We are very grateful to Dr. Zhu L. Yang and Dr. R. H. Petersen for providing us with specimens, reviewing the manuscript, and giving valuable suggestions. We also thank Dr. Xiang-Hua Wang for providing valuable materials used in this study; the herbarium of the National Botanical Garden of Belgium for sending us Boletus specimens on loan; the University of Tennessee Herbarium and Herbarium WU, Institute of Botany, University of Vienna, for lending us specimens on loan and permitting us to extract DNA from the materials; and the Herbarium of Cryptogams, Kunming Institute of Botany, Chinese Academy of Sciences, for providing us B. speciosus specimens on loan. We greatly appreciate the assistance of Ms. Xiaoxia Sun and Dr Hepeng Li (Zhejiang Forestry Academy) in the preparation of the line-drawings.

References

  1. Assyov B (2012) Revision of Boletus section Appendiculati (Boletaceae) in Bulgaria with a key to the Balkan species. Turk J Bot 36:408–419Google Scholar
  2. Assyov B, Gyosheva MM (2010) First encounters of Boletus subappendiculatus (Boletaceae) in Bulgaria. Phytol Balc 16(3):323–327Google Scholar
  3. Beugelsdijk DCM, Van Der Linde S, Zuccarello GC, Den Bakker HC, Draisma SGA, Noordeloos ME (2008) A phylogenetic study of Boletus section Boletus in Europe. Persoonia 20:1–7PubMedCentralPubMedCrossRefGoogle Scholar
  4. Binder M, Bresinsky A (2002) Retiboletus, a new genus for a species-complex in the Boletaceae producing retipolides. Feddes Repertorium 113(1–2):30–40CrossRefGoogle Scholar
  5. Binder M, Hibbett DS (2006) Molecular systematics and biological diversification of Boletales. Mycologia 98(6):971–981PubMedCrossRefGoogle Scholar
  6. Castresana J (2000) Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol 17:540–552PubMedCrossRefGoogle Scholar
  7. Chiu WF (1948) The Boletes of Yunan. Mycologia 40:199–231CrossRefGoogle Scholar
  8. Den Bakker HC, Gravendeel B, Kuyper TW (2004) An ITS phylogeny of Leccinum and an analysis of the evolution of minisatellite-like sequences within ITS1. Mycologia 96(1):102–118CrossRefGoogle Scholar
  9. Dentinger BTM, Ammirati JF, Both EE, Desjardin DE, Halling RE, Henkel TW, Moreau PA, Nagasawa E, Soytong K, Taylor AF, Watling R, Moncalvo JM, McLaughlin DJ (2010) Molecular phylogenetics of porcini mushrooms (Boletus section Boletus). Mol Phylogenet Evol 57:1276–1292PubMedCrossRefGoogle Scholar
  10. Eyssartier G, Stubbe D, Walleyn R, Verbeken A (2009) New records of Cantharellus species (Basidiomycota, Cantharellaceae) from Malaysian dipterocarp rainforest. Fungal Divers 36:57–67Google Scholar
  11. Felsenstein J (1981) Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol 17(6):368–376PubMedCrossRefGoogle Scholar
  12. Feng B, Xu J, Wu G, Hosen MI, Zeng NK, Li YC, Tolgor B, Kost GW, Yang ZL (2012) DNA sequence analyses reveal abundant diversity, endemism and evidence for Asian origin of the porcini mushrooms. PLoS One 7(5):e37567PubMedCentralPubMedCrossRefGoogle Scholar
  13. Kimura M (1980) A simple method for estimating evolutionary rates of nucleotide substitution through comparative studies of nucleotide sequences. J Mol Evol 16:111–120PubMedCrossRefGoogle Scholar
  14. Leonardi M, Paolocci F, Rubini A, Simonini G, Pacioni G (2005) Assessment of inter- and intra-specific variability in the main species of Boletus edulis complex by ITS analysis. FEMS Microbiol Lett 243:411–416PubMedCrossRefGoogle Scholar
  15. Li HB, Wu XQ, Wei HL, Fu LZ, Wu QQ, Chen YL, Fan LM, Liu Q, Wu CL, Wu XP (2005) Brief report on investigation of ectomycorrhizal fungi resources in Lishui area, Zhejiang province(in Chinese). Edible Fungi China 24(5):10–14Google Scholar
  16. Li HB, Wu XQ, Ying GH, Wei HL, Fu LZ, Wu QQ, Xu L, Liu Q (2007) Authentication of fungal mycelium isolated from Boletus appendiculatus fruit body tissue using RAPD and ITS markers (in Chinese). Acta Edulis Fungi 12(1):46–50Google Scholar
  17. Li YC, Yang ZL, Tolgor B (2009) Phylogenetic and biogeographic relationships of Chroogomphus species as inferred from molecular and morphological data. Fungal Divers 38:85–104Google Scholar
  18. Manian S, Sreenivasaprasad S, Bending GD, Mills PR (2001) Genetic diversity and interrelationships among common European Suillus species based on ribosomal DNA sequences. FEMS Microbiol Lett 204(1):117–121PubMedCrossRefGoogle Scholar
  19. Mao XL (2000) The macrofungi in China (in Chinese). Henan Science and Technology, ZhengzhouGoogle Scholar
  20. Möller EM, Bahnweg G, Sandermann H, Geiger HH (1992) A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res 20:6115–6116PubMedCentralPubMedCrossRefGoogle Scholar
  21. Muñoz JA (2005) Boletus s.l. In: Fungi Europaei. Vol. 1. Candusso, AlassioGoogle Scholar
  22. Singer R (1986) The Agaricales in modern taxonomy, 4th edn. Koeltz, KönigsteinGoogle Scholar
  23. Smith JE, Lebel T (2001) A comparison of taxonomic keys to species within the genus Russula. Mcllvainea 15(1):9–22Google Scholar
  24. Smith AH, Thiers HD (1971) The Boletes of Michigan. University of Michigan Press, Ann Arbor, p 428Google Scholar
  25. Takehashi S, Kasuya T (2009) Pluteus magnus and Pluteus podospileus f. podospileus, two agaric species new to Japan. Mycoscience 50:74–77CrossRefGoogle Scholar
  26. Tamura K, Peterson D, Peterson N, Stecher G, Nei M, Kumar S (2011) MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol 28(10):2731–2739PubMedCrossRefGoogle Scholar
  27. Thompson JD, Gibson TJ, Plewniak F, Higgins DG (1997) The Clustal X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25(24):4876–4882PubMedCentralPubMedCrossRefGoogle Scholar
  28. Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. J Bacteriol 172:4238–4246PubMedCentralPubMedGoogle Scholar
  29. Watling R, Hills AE, Turnbull E (2005) Boletes and their allies (revised and enlarged edition). In: Henderson DM, Orton PD, Watling R (eds) British fungus flora. Agarics and boleti, vol 1. Royal Botanic Garden, EdinburghGoogle Scholar
  30. White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, San Diego, pp 315–322Google Scholar
  31. Yang ZL (1997) Die Amanita-Arten von Südwestchina. Bibl Mycol 170:1–240Google Scholar
  32. Yang ZL (2005) Diversity and biogeography of higher fungi in China. In: Xu J (ed) Evolutionary genetics of fungi. Horizon, Norfolk, pp 35–62Google Scholar
  33. Ying GH, Lv ML, Chen YL, Mei JW, Jia YN, Gu XW (2005) The current situation of Boletus appendiculatus picking and the countermeasures (in Chinese). Acta Edulis Fungi 12(1):46–50Google Scholar
  34. Zang M (2006) Flora fungorum sinicorum Boletaceae (I), vol 22. Science Press, Beijing, pp 49–50, 143–144 (in Chinese)Google Scholar
  35. Zeng NK, Tang LP, Li YC, Tolgor B, Zhu XT, Zhao Q, Yang ZL (2013) The genus Phylloporus (Boletaceae, Boletales) from China: morphological and multilocus DNA sequence analyses. Fungal Divers. doi: 10.1007/s13225-012-0184-7

Copyright information

© German Mycological Society and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Haibo Li
    • 1
    Email author
  • Hailong Wei
    • 1
  • Huazheng Peng
    • 1
  • Hongmei Ding
    • 2
  • Liling Wang
    • 1
  • Liang He
    • 1
  • Lizhong Fu
    • 1
  1. 1.Zhejiang Forestry AcademyHangzhouChina
  2. 2.Zhejiang Chinese Medical UniversityHangzhouChina

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