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Development of SSR markers for typing cultivars in the mushroom Auricularia auricula-judae

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Abstract

Auricularia auricula-judae, also known as black wood ear, is one of the most popular edible mushrooms in China. But the confusion about cultivars has limited the development of A. auricula-judae production. In this article, 17 polymorphic SSR markers were cloned and used to differentiate the cultivars of A. auricula-judae. The polymorphism information content (PIC) of these SSR ranged from 0.10 to 0.84, while the average was 0.47. The number of alleles detected for each locus was 2–11, with an average of 4.7 alleles per locus. The dendrogram, based on 17 SSR markers by UPGMA clustering, could differentiate the 16 A. auricula-judae cultivars in this study. In fact, the 16 cultivars analyzed in this study could be efficiently differentiated using a combination of three polymorphic SSR loci with high PIC. The total of 17 polymorphic SSR loci could also be amplified correctly in the A. polytricha strains surveyed. This is the first report on the development of SSR markers in the genus Auricularia.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31101588), and the China agriculture research system (No.CARS-24).

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Authors and Affiliations

  1. Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture, and Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Rui Ying Zhang, Jin Gang Gu, Qing Xiu Hu & Xue Mei Zuo

  2. Beijing Academy of Science and Technology, Beijing, 100089, China

    Dan Dan Hu

  3. State Key Laboratory for Agrobiotechnology and Department of Microbiology, China Agricultural University, Beijing, 100094, China

    He Xiang Wang

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  1. Rui Ying Zhang
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  2. Dan Dan Hu
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  3. Jin Gang Gu
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  4. Qing Xiu Hu
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  5. Xue Mei Zuo
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Correspondence to Rui Ying Zhang.

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Zhang, R.Y., Hu, D.D., Gu, J.G. et al. Development of SSR markers for typing cultivars in the mushroom Auricularia auricula-judae . Mycol Progress 11, 587–592 (2012). https://doi.org/10.1007/s11557-011-0798-2

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  • DOI: https://doi.org/10.1007/s11557-011-0798-2

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