Two new Pythium species from China based on the morphology and DNA sequence data
- 376 Downloads
In an investigation of Pythium species in lawn grassland of south China, two new species, Pythium breve and P. baisense, were identified based on morphological characteristics and DNA sequence data. These two new species differ morphologically from the other Pythium species by the oogonia encompassed by many antheridia and with 1–3 antheridia on the wavy and curved stalks. Furthermore, P. baisense with complexly lobed antheridial stalks differs from P. breve with antheridial stalks entwining the hyphae nearby the oogonia with several turns. Results of phylogenetic analyses showed that these two new species were clearly separated from morphologically similar Pythium species based on the internal transcribed spacer region (ITS1, 5.8S, ITS2) and cytochrome c oxidase subunit I (COXI) gene sequences using maximum parsimony and Bayesian methods. These two new species are described and illustrated in detail.
KeywordsPythium breve Pythium baisense Morphology ITS COXI
This work was supported by the National Natural Science Foundation of China (No. 30670012), the Guangxi Natural Science Foundation (No. 0640013) and the Plans for Construction of Scientific Topnotch and Innovation Team in Guangxi University.
- Bala K, Robideau GP, Lévesque CA, de Cock AWAM, Abad ZG, Lodhi AM, Shahzad S, Ghaffar A, Coffey MD (2010b) Phytopythium Abad, de Cock, Bala, Robideau & Lévesque, gen. nov. and Phytopythium sindhum Lodhi, Shahzad & Lévesque, sp. nov. Persoonia 24:136–137Google Scholar
- Belbahri L, McLeod A, Paul B, Calmin G, Moralejo E, Spies CFJ, Botha WJ, Clemente A, Descals E, Sánchez-Hernández E, Lefort F (2008) Intraspecific and within-isolate sequence variation in the ITS rRNA gene region of Pythium mercuriale sp. nov. (Pythiaceae). FEMS Microbiol Lett 284:17–27PubMedCrossRefGoogle Scholar
- Galland D, Paul B (2001) Pythium perplexum isolated from soil in France: morphology, molecular characterisation and biological control. Mycol Res 156:185–189Google Scholar
- Long YY, Wei JG, Huang CL, He YQ, Yuan GQ, Shi Y, Xiong Y (2010) A new Pythium species isolated from vegetable fields and analysis by rDNA ITS sequence. Mycosystema 29(6):795–800Google Scholar
- Nylander JAA (2008) MrModeltest 2.3 README. http://www.abc.se/~nylander/mrmodeltest2/mrmodeltest2.html. Accessed 22 May 2008
- Paul B (1991) Pythium folliculosum, a new species from the bank of Lake Zǖrich. Mycol Helvetica 4:203–208Google Scholar
- Swofford DL (2002) Phylogenetic Analysis Using Parsimony (and other methods). Version 4, Sinauer, Sunderland, Mass. http://paup.csit.fsu.edu/Cmd_ref_v2.pdf
- Van der Plaats-Niterink AJ (1981) Monograph of the genus Pythium. Stud Mycol 21:1–242Google Scholar
- White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: Innis MA, Sninsky DH, White TJ (eds) PCR Protocols. Academic, London, pp 315–322Google Scholar
- Yu YN (1998) Peronosporales [Flora Fungorum Sinicorum Vol. 6]. Science Press, Beijing (in Chinese)Google Scholar