Abstract
The genus Albugo s.str. causes white blister rust on four families of the Brassicales, Brassicaceae, Capparaceae, Cleomaceae, and Resedaceae. Recent phylogenetic studies have revealed that several host specific lineages are present within Albugo on Brassicales, while it was also confirmed that Albugo candida has an exceptionally wide host range which extends from Brassicaceae to Cleomaceae and Capparaceae. The Albugo species infecting the Resedaceae was attributed in monographic studies as well as local floras to either A. resedae or, applying a broader species concept, to A. candida. In the present study, A. resedae specimens were morphologically and molecularly compared to the five Albugo species so far confirmed from Brassicales, A. candida, A. koreana, A. laibachii, A. lepidii, and A. voglmayrii. Both morphological differences of oospore ornamentation and phylogenetic analysis of cox2 mtDNA sequences provided evidence that A. resedae is distinct from A. candida and from the additional four species so far described from Brassicaceae. It thus seems possible that so far unknown factors restrict Albugo candida to Brassicaceae and its sister families, Cleomaceae and Capparaceae.
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Angiosperm Phylogeny Group (APG) (1998) An ordinal classification for the families of flowering plants. Ann Mo Bot Gard 85:531–553
Angiosperm Phylogeny Group II (APG II) (2003) An update of the Angiosperm Phylogeny Group classification for the orders and families of flowering plants: APG II. Bot J Linn Soc 141:399–436
Biga MLB (1955) Review of the species of the genus Albugo based on the morphology of the conidia. Sydowia 9:339–358
Choi D, Priest MJ (1995) A key to the genus Albugo. Mycotaxon 53:261–272
Choi YJ, Hong SB, Shin HD (2006) Genetic diversity within the Albugo candida complex (Peronosporales, Oomycota) inferred from phylogenetic analysis of ITS rDNA and COX2 mtDNA sequences. Mol Phylogenet Evol 40:400–409
Choi YJ, Shin HD, Hong SB, Thines M (2007) Morphological and molecular discrimination among Albugo candida materials infecting Capsella bursa-pastoris world-wide. Fungal Divers 27:11–34
Choi YJ, Shin HD, Ploch S, Thines M (2008) Evidence for uncharted biodiversity in the Albugo candida complex, with the description of a new species. Mycol Res 112:1327–1334
Choi YJ, Shin HD, Thines M (2009) The host range of Albugo candida extends from Brassicaceae over Cleomaceae to Capparaceae. Mycol Prog 8:329–335
Felsenstein J (1985) Confidence limits on phylogenies: an approach using the Bootstrap. Evolution 39:783–791
Hall JC, Sytsma KJ, Iltis HH (2002) Phylogeny of Capparaceae and Brassicaceae based on chloroplast sequence data. Am J Bot 89:1826–1842
Hall JC, Iltis HH, Sytsma KJ (2004) Molecular phylogenetics of core Brassicales, placement of orphan genera Emblingia, Forchhammeria, Tirania, and character evolution. Syst Bot 29:654–669
Hudspeth DSS, Nadler SA, Hudspeth MES (2000) A COX2 molecular phylogeny of the peronosporomycetes. Mycologia 92:674–684
Hudspeth DSS, Stenger D, Hudspeth MES (2003) A COX2 phylogenetic hypothesis of the downy mildews and white rusts. Fungal Divers 13:47–57
Jaczewski AA, Jaczewski PA (1931) OpredeliteV Gribov. Sovershennye Griby (diploidnye stadii). Tom I. Fikomitsety, ed. 3. Sel'kolkhozgiz, Moskva-Leningrad [In Russian]
Jörstad I (1964) The Phycomycetous genera Albugo, Bremia, Plasmopara, and Pseudoperonospora in Norway, with an appendix containing unpublished finds of Peronospora. Nytt Mag Bot 11:47–82
Khunti JP, Khandar RR, Bhoraniya MF (2000) Studies on host range of Albugo cuciferarum the incitant of white rust of mustard. Agric Sci Dig 20:219–221
Lee SB, Taylor JW (1990) Isolation of DNA from fungal mycelia and single spores. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications. Academic, New York, pp 282–287
Martín-Bravoa S, Meimberg H, Luceño M, Märkl W, Valcárcel V, Bräuchler C, Vargas P, Heubl G (2007) Molecular systematics and biogeography of Resedaceae based on ITS and trnL-F sequences. Mol Phylogenet Evol 44:1105–1120
Melchers LE (1931) A check list of plant disease and fungi occurring in Egypt. Trans Kans Acad Sci 34:41–106
Mukerji KG (1975) Albugo candida. CMI Descriptions of Pathogenic Fungi and Bacteria No. 460
Novotelnova NS, Pystina KA (1985) Ordo Peronosporales (families Pythiaceae, Phytophthoraceae, Peronosporaceae, Cystopaceae) [Cryptogamic Flora of USSR, vol. 11 (3)], Nauka, Leningrad
Priest MJ (1996) Albugo spp. on the Gentianaceae. Mycotaxon 58:69–76
Riethmüller A, Voglmayr H, Göker M, Weiß M, Oberwinkler F (2002) Phylogenetic relationships of the downy mildews (Peronosporales) and related groups based on nuclear large subunit ribosomal DNA sequences. Mycologia 94:834–849
Saharan GS, Verma PR (1992) White rusts: a review of economically important species. International Development Research Centre, Ottawa, Ont
Stamatakis A (2006) RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics 22:2688–2690
Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599
Telle S, Thines M (2008) Amplification of cox2 (~620 bp) from 2 mg of up to 129 years old herbarium specimens, comparing 19 extraction methods and 15 polymerases. PLoS ONE 3:e3584
Thines A, Spring O (2005) A revision of Albugo (Chromista, Peronosporomycetes). Mycotaxon 92:443–458
Thines M, Voglmayr H (2009) An introduction to the white blister rusts (Albuginaceae) In: Lamour K, Kamoun S (eds) Oomycete genetics and genomics: biology, interactions with plants and animals, and toolbox. Wiley-Blackwell, pp 77–92
Thines M, Choi YJ, Kemen E, Ploch S, Holub EB, Shin HD, Jones JDG (2009) A new species of Albugo parasitic to Arabidopsis thaliana reveals new evolutionary patterns in white blister rusts (Albuginaceae). Persoonia 22:123–128
Ul’yanishchev VI, Osipian LL, Kanchaveli LA, Akhundov TM (1985) Peronosporovye Griby. [Peronosporaceous fungi] In: Ul’yanishchev VI (ed) Opredelitel’ gribov Zakavkaz’ya Erevan University, Erevan
Voglmayr H, Riethmüller A (2006) Phylogenetic relationships of Albugo species (white blister rusts) based on LSU rDNA sequence and oospore data. Mycol Res 110:75–85
Acknowledgments
The authors are grateful to the curators of BPI and GZU for providing the Albugo specimens investigated in this study. The present study was financially supported by the research funding programme “LOEWE–Landes-Offensive zur Entwicklung Wissenschaftlich-ökonomischer Exzellenz” of Hesse's Ministry of Higher Education, Research, and the Arts, as well as by a grant in the Elite Program for Postdocs of the Landesstiftung Baden-Württemberg awarded to MT.
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Choi, YJ., Thines, M. Morphological and molecular confirmation of Albugo resedae (Albuginales; Oomycota) as a distinct species from A. candida . Mycol Progress 10, 143–148 (2011). https://doi.org/10.1007/s11557-010-0683-4
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DOI: https://doi.org/10.1007/s11557-010-0683-4