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Mycological Progress

, Volume 9, Issue 4, pp 469–484 | Cite as

The delimitation of Flammulina fennae

  • Soňa Ripková
  • Karen Hughes
  • Slavomír Adamčík
  • Viktor Kučera
  • Katarína Adamčíková
Original article

Abstract

Multivariate morphometric analyses of micromorphological characters measured on 35 specimens of Flammulina fennae and related species show that only a combination of spore dimensions and ixohyphidia characters are suitable for delimitation of this species. In order to confirm species identifications based on micromorphology, ribosomal ITS DNA sequences were obtained and compared with those previously deposited in GenBank, and phylogenetic analyses were performed using an ITS dataset of all known Flammulina species. All six specimens morphologically determined as F. fennae were identified by molecular data. Two of twelve specimens morphologically assigned to F. velutipes had F. elastica sequences. One ITS sequence of F. velutipes appears to be a hybrid: the ITS1 region is homologous to F. velutipes and ITS2 is homologous to another Flammulina species, F. rossica. Variability of morphocharacters observed for F. fennae and related species is discussed and compared with the data from previous studies. A key is provided to European taxa of the genus Flammulina together with a full description of F. fennae.

Keywords

Flammulina velutipes F. ononidis Flammulina hybrid Multivariate morphometrics ITS sequences 

Notes

Acknowledgements

Jan Borovička, Ladislav Hagara, Per Marstad, and Anna Ronikier are acknowledged for providing us with additional material of Flammulina fennae. We are grateful to Dagmar Triebel, who corrected information rewritten from the original hand-written text on herbarium sheets of specimens from herbarium M. The Slovak Grant Agency VEGA (grant no. 2/7071/7 and 2/5087/25) supported this study.

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Copyright information

© German Mycological Society and Springer 2010

Authors and Affiliations

  • Soňa Ripková
    • 1
  • Karen Hughes
    • 2
  • Slavomír Adamčík
    • 3
  • Viktor Kučera
    • 3
  • Katarína Adamčíková
    • 4
  1. 1.Faculty of Natural Sciences, Department of BotanyComenius University in BratislavaBratislavaSlovakia
  2. 2.Ecology and Evolutionary BiologyUniversity of TenneseeKnoxvilleUSA
  3. 3.Institute of Botany SASBratislavaSlovakia
  4. 4.Dúbravská Organization Branch for Woody Plants Biology NitraInstitute of Forest Ecology SASNitraSlovakia

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