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Phylogeny and taxonomy of powdery mildew fungi of Erysiphe sect. Uncinula on Carpinus species


A phylogenetic analysis of the Erysiphe with uncinuloid ascoma appendages (Erysiphe section Uncinula, Erysiphales, Ascomycota) on Carpinus spp. was done using sequences of the rDNA ITS regions and the D1/D2 domains of the 28S rDNA. These results, combined with morphological data, revealed a complex consisting of several distinct taxa. These included the already described Erysiphe carpinicola on C. japonica distinguishable from the Erysiphe sp. on C. betulus and C. tschonoskii as well as the one on C. laxiflora. Thus, it was shown that Oidium carpini, described from Europe on Carpinus betulus, the powdery mildew with uncinula-like ascomata, recently found in Europe on this host, as well as an Erysiphe on C. tschonoskii in Japan, described previously as E. carpinicola, all belong to a single new species, named E. arcuata in this paper. As the powdery mildew on C. laxiflora was also distinct from other known species, it is named E. carpini-laxiflorae in this paper. The already described E. pseudocarpinicola and Erysiphe sp. on Carpinus cordata are two additional taxa, which are morphologically and genetically distinguished from the other species of Erysiphe sect. Uncinula on Carpinus spp.

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  • Amano K (1986) Host range and geographical distribution of the powdery mildew fungi. Japan Scientific Societies, Tokyo

    Google Scholar 

  • Bolay A (2005) Les Oïdiums de Suisse (Erysiphaceae). Cryptogam Helv 20:1–173

    Google Scholar 

  • Braun U (1987) A monograph of the Erysiphales (powdery mildews). Nova Hedwigia Beih 89:1–700

    Google Scholar 

  • Braun U (1995) The powdery mildews (Erysiphales) of Europe. G. Fischer, Jena

    Google Scholar 

  • Braun U (1999) Some critical notes on the classification and generic concept of the Erysiphaceae. Schlechtendalia 3:49–55

    Google Scholar 

  • Braun U, Takamatsu S (2000) Phylogeny of Erysiphe, Microsphaera, Uncinula (Erysipheae) and Cystotheca, Podosphaera, Sphaerotheca (Cystotheceae) inferred from rDNA ITS sequences—some taxonomic consequences. Schlechtendalia 4:1–33

    Google Scholar 

  • Bunkina IA (1991) Porjadok Erysiphales Gwinne-Vaughan. In: Azbukina ZM (ed) Nizshie rastenija, griby i mohoobraznye Sovetskogo Dal’nego Vostoka, Griby, Tom 2, Askomicety, Erizifal’nye, Klavicipital’nye, Gelocial’nye. Nauka, Leningrad, pp 11–142

    Google Scholar 

  • Chen GQ, Han SJ, Lai YQ, Yu YN, Zheng RY, Zhao ZY (1987) Flora fungorum sinicorum, vol. 1, Erysiphales. Science, Beijing

    Google Scholar 

  • Chen ZD, Manchester SR, Sun HY (1999) Phylogeny and evolution of the Betulaceae as inferred from DNA sequences, morphology, and paleobotany. Am J Bot 86:1168–1181

    PubMed  Article  CAS  Google Scholar 

  • Conert HJ, Hamann U, Schulze-Motel W, Wagenitz G (1981) Gustav Hegi’s Illustrierte Flora von Mitteleuropa, Band III, Teil 1. Paul Parey Verlag, Berlin–Hamburg

    Google Scholar 

  • Cook RTA, Inman AJ, Billings C (1997) Identification and classification of powdery mildew anamorphs using light and scanning electron microscopy and host range data. Mycol Res 101:975–1002

    Article  Google Scholar 

  • Czerepanov SK (1995) Plantae vasculares Rossicae et civitatum collimitanearum (in limicis URSS olim). Mir i Sem’ja, St. Petersburg

  • Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  • Fang ZF, Zhao S, Skvortsov AK (1999) Betulaceae. In: Wu ZY, Raven PH (eds) Flora of China, Cycadaceae through Fagaceae. Science, Beijing and Missouri Botanical Garden, St. Louis, pp 286–313

    Google Scholar 

  • Hasegawa M, Kishino H, Yano TA (1985) Dating of the human–ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174

    PubMed  Article  CAS  Google Scholar 

  • Heluta VP [Geluta WP] (1989) Flora gribov Ukrainy. Muchnistorosyanye griby. Naukova Dumka, Kiev

  • Hirata T, Takamatsu S (1996) Nucleotide sequence diversity of rDNA internal transcribed spacers extracted from conidia and cleistothecia of several powdery mildew fungi. Mycoscience 37:265–270

    Article  Google Scholar 

  • Holmgren PK, Holmgren NH, Barbett LC (1990) Index herbariorum, Part. 1: The herbaria of the world, 8th edn. Regnum veg 120:1–163

    Google Scholar 

  • Horikawa Y (1972) Atlas of the Japanese flora, an introduction to plant sociology of East Asia. Gakken, Tokyo

    Google Scholar 

  • Huelsenbeck JP, Ronquist F (2001) MrBayes: Bayesian inference of phylogenetic trees. Bioinformatics 17:754–755

    PubMed  Article  CAS  Google Scholar 

  • Kishino H, Hasegawa H (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and the branching order in Hominoidea. J Mol Evol 29:170–179

    PubMed  Article  CAS  Google Scholar 

  • Kusaba M, Tsuge T (1995) Phylogeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA. Curr Genet 28:491–498

    PubMed  Article  CAS  Google Scholar 

  • Maddison DR, Maddison WP (2002) MacClade 4: analysis of phylogeny and character evolution. Version 4.05. Sinauer Associates, Sunderland, Massachusetts

  • Mori Y, Sato Y, Takamatsu S (2000a) Evolutionary analysis of the powdery mildew fungi using nucleotide sequences of the nuclear ribosomal DNA. Mycologia 92:74–93

    Article  CAS  Google Scholar 

  • Mori Y, Sato Y, Takamatsu S (2000b) Molecular phylogeny and radiation time of Erysiphales inferred from the nuclear ribosomal DNA sequences. Mycoscience 41:437–447

    Article  CAS  Google Scholar 

  • Nomura Y (1992) Erysiphaceae of Japan. Yotsukaido C., Japan (published by the author)

  • Nomura Y (1997) Taxonomical study of Erysiphaceae of Japan. Yokendo, Japan

    Google Scholar 

  • Nylander JAA (2004) MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University, Sweden

  • Pitek M (2004) First report of powdery mildew (Oidium carpini) on Carpinus betulus in Poland. New Dis Rep 8, Aug. 2003–Jan. 2004 []

  • Rancovic B (2003) Powdery mildew fungi (order Erysiphales) on plants in Montenegro (Chernogoria). Mikol Fitopatol 37:42–52

    Google Scholar 

  • Scheuer C (2003) Mykofloristische Erkenntnisse der Exkursion zur Lehrveranstaltung BIODIVERSITÄT DER PILZE (VE) am 18. und 25 Oktober 2003 [ html]

  • Shimodaira H, Hasegawa M (1999) Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol 16:1114–1116

    CAS  Google Scholar 

  • Shin HD (2000) Erysiphaceae of Korea. Plant Pathogens of Korea 1. National Institute of Agricultural Science and Technology, Suwon, Korea

    Google Scholar 

  • Siebold PF, Zuccarini JG (1846) Florae japonicae familiae naturales. II. München

  • Simonyan SA (1994) Mikoflora Armenii. VII. Muchnistorosyanye griby Armenii (por. Erysiphales). Izdatelstvo AN Armenii, Yerevan

  • Swofford DL (2001) PAUP: Phylogenetic Analysis Using Parsimony (and other methods) 4.0b8. Sinauer, Sunderland, MA, USA

  • Szabó I (2003) Leaf pathogenic fungi of forest trees and shrubs in Hungary. Fritschiana 42:67–70

    Google Scholar 

  • Tai FL (1936) Notes on Chinese fungi VI. Additional notes on Erysiphaceae of China. Bull Chin Bot Soc 2:16–28

    Google Scholar 

  • Tai FL (1979) Sylloge fungorum sinicorum. Science, Academica Sinica, Peking

    Google Scholar 

  • Takamatsu S, Kano Y (2001) PCR primers useful for nucleotide sequencing of rDNA of the powdery mildew fungi. Mycoscience 42:135–139

    Article  CAS  Google Scholar 

  • Takamatsu S, Braun U, Limkaisang S (2005a) Phylogenetic relationships and generic affinity of Uncinula septata inferred from nuclear rDNA sequences. Mycoscience 46:9–16

    Article  CAS  Google Scholar 

  • Takamatsu S, Niinomi S, Cabrera De Álvarez MG, Álvarez RE, Havrylenko M, Braun U (2005b) Caespitotheca gen. nov., an ancestral genus in the Erysiphales. Mycol Res 109:903–911

    PubMed  Article  Google Scholar 

  • Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

    Article  Google Scholar 

  • To-anun C, Kom-un S. Limkaisang S, Fangfuk W, Sato Y, Takamatsu S (2005) A new subgenus, Microidium, of Oidium (Erysiphaceae) on Phyllanthus spp. Mycoscience 46:1–8

    Article  CAS  Google Scholar 

  • Vajna L (2005) Powdery mildew caused by Erysiphe carpinicola on Carpinus betulus in Hungary: first European report. New Dis Rep 12, Aug. 2005–Jan. 2006 []

  • Walsh PS, Metzger DA, Higuchi R (1991) Chelex 100 as a medium for simple extraction of DNA for PCR-based typing from forensic material. BioTechniques 10:506–513

    PubMed  CAS  Google Scholar 

  • White TJ, Bruns TD, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal genes for phylogenetics. In: Innis MS, Gelfand DH, Sninsky JJ, White TJ (eds) PCR protocols: a guide to methods and applications, Academic, San Diego, California, pp 315–322

    Google Scholar 

  • Wołczańska A (2006) First report of Erysiphe carpinicola s.l. (perfect state) in Poland. New Dis Rep (in press)

  • Yoo KO, Wen J (2002) Phylogeny and biogeography of Carpinus and subfamily Coryloideae (Betulaceae). Int J Plant Sci 163:641–650

    Article  Google Scholar 

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Since thanks are due to J. Fischer (Freiberg, Germany), H. Jage (Kemberg, Germany), V. Kummer (Potsdam, Germany), L. Vajna (Budapest, Hungary), S. Voytyuk (Kiev, Ukraine) and A. Wołczañska (Lublin, Poland) for kindly providing teleomorph collections on Carpinus betulus, to L. Kiss (Plant Protection Institute, Hungarian Academy of Sciences, Budapest) for his kind help and comments on this study, to D. P. Diomenko (laboratory of electron microscopy of M.G. Kholodny Institute of Botany, Ukraine), and I. Taylor (Central Science Laboratory, York, UK) for their help with scanning electron microscopy.

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Correspondence to Uwe Braun.

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Braun, U., Takamatsu, S., Heluta, V. et al. Phylogeny and taxonomy of powdery mildew fungi of Erysiphe sect. Uncinula on Carpinus species. Mycol Progress 5, 139–153 (2006).

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Taxonomical Novelties

  • Erysiphe arcuata
  • Erysiphe carpini-laxiflorae


  • Erysiphales
  • Erysiphe sect. Uncinula
  • Erysiphe carpinicola
  • new species
  • sequence analyses
  • morphology