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Identification of Picea-ectomycorrhizae by comparing DNA-sequences

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Abstract

Identification of the fungi forming ectomycorrhiza is still a great challenge. Ectomycorrhizae of Picea abies, collected in southwest Germany during several years and described as morphotypes, were identified using LSU and ITS sequences. To this the fungal sequences amplified from the mycorrhizae were compared with sequences from identified sporocarps. The fungal partner of Piceirhiza gelatinosa was identified as Hygrophorus olivaceoalbus, the fungal partner of Piceirhiza rosa-nigrescens was identified as Dermocybe cf. semisanguinea, and the fungal partner of a white mycorrhiza, described here for the first time, belongs to the Hebeloma velutipes group (Hebeloma crustuliniforme complex). Identification to genus level was possible for Piceirhiza lanuginosa where a Cortinarius-species is the fungal partner. A Tomentella-species forms a newly described light-brown mycorrhiza. Identification to family and to order-level was achieved for a milky-dull silvery mycorrhiza (Cortinariaceae), and Piceirhiza globulifera (Aphyllophorales), respectively. Ten samples of white, slightly bent mycorrhizae were formed by 8 different Cortinarius-species, including among others Cortinarius traganus, C. delibutus, and C. brunneus. The fungal partners of several brown, smooth mycorrhizae displaying only a Hartig net are Ascomycetes, among these are Wilcoxina cf. mikolae and Hymenoscyphus cf. ericae.

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References

  • Aanen DK, Kuyper TW, Boekhout T, Hoekstra RF (2000) Phylogenetic relationships in the genus Hebeloma based on ITS1 and ITS2 sequences, with special emphasis on the Hebeloma crustuliniforme complex. — Mycologia 92: 269–281.

    CAS  Google Scholar 

  • Aanen DK, Kuyper TW, Hoekstra RF (2001) An extensive and widely distributed ITS polymorphism within a biological species of the ectomycorrhizal fungus Hebeloma velutipes. — Mycological Research 105: 284–290.

    Article  CAS  Google Scholar 

  • Agerer R, ed. (1987–1998) Colour atlas of ectomycorrhizae, 1st–11th edn. Einhorn, Schwäbisch Gmünd.

    Google Scholar 

  • Agerer R (1995) Anatomical characteristics of identified ectomycorrhizas: an attempt towards a natural classification. In: Varma A, Hock B (eds) Mycorrhiza — Structure, Function, Molecular Biology and Biotechnology, pp 685–734.

  • Altschul, S F, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic Local Alignment Search Tool. — Journal of Molecular Biology 215: 403–410.

    Article  CAS  PubMed  Google Scholar 

  • Baar J, Horton TR, Kretzer AM, Bruns TD (1999) Mycorrhizal colonization of Pinus muricata from resistant propagules after a stand-replacing wildfire. — New Phytologist 143: 409–418.

    Article  Google Scholar 

  • Bas C, Kuyper ThW, Nordeloss ME, Vellinga EC (eds) (1990) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands. A.A.Balkema/Rotterdam/Brookfield.

  • Brand F (1991) Ektomykorrhizen an Fagus sylvatica. Charakterisierung und Identifizierung, ökologische Kennzeichnung und unsterile Kultivierung. — Libri Botanici 2: 1–229.

    Google Scholar 

  • Brunner I, Amiet R, Schneider B (1991) Characterization of naturally grown and in vitro synthesized ectomycorrhizas of Hebeloma crustuliniforme and Picea abies. — Mycological Research 95: 1407–1413.

    Google Scholar 

  • Bruns TD, Szaro TM, Gardes M, Cullings KW, Pan JJ, Taylor DL, Horton TR, Kretzer A, Garbeletto M, Li Y (1998) A sequence database for the identification of ectomycorrhizal basidiomycetes by phylogenetic analysis. — Molecular Ecology 7: 257–272.

    Article  CAS  Google Scholar 

  • Dahlberg A, Jonsson L, Nylund JE (1997) Species diversity and distribution of biomass above and below ground among ectomycorrhizal fungi in an old-growth Norway spruce forest in south Sweden. — Canadian Journal of Botany 75: 1323–1335.

    Google Scholar 

  • Eberhardt U, Walter L, Kottke I (1999) Molecular and morphological discrimination between Tylospora fibrillosa and Tylospora asterophora mycorrhizae. — Canadian Journal of Botany 77: 11–21.

    Article  CAS  Google Scholar 

  • Einsele G (ed)(1986) Das landschaftsökologische Forschungsprojekt Naturpark Schönbuch. Forschungsbericht VCH, 636 pp.

  • Erland S, Jonsson T, Mahmood S, Finlay RD (1999) Below-ground ectomycorrhizal community structure in two Picea abies forets in southern Sweden. — Scandinavian Journal of Forest Research 14: 209–217.

    Google Scholar 

  • Fransson PMA, Taylor AFS, Finlay RD (2000) Effects of continous optimal fertilization on below-ground ectomycorrhizal community structure in a Norway spruce forest. — Tree Physiology 20: 599–606.

    PubMed  Google Scholar 

  • Gardes M, Bruns T (1993) ITS primers with enhanced specifity for Basidiomycetes: application to the identification of mycorrhizae and rusts. — Molecular Ecology 2: 113–118.

    CAS  PubMed  Google Scholar 

  • Gardes M, White TJ, Fortin JA, Bruns TD, Taylor JW (1991) Identification of indigenous and introduced symbiotic fungi in ectomycorrhizae by amplification of nuclear and mitochondrial ribosomal DNA. — Canadian Journal of Botany 69: 180–190.

    CAS  Google Scholar 

  • Gronbach E (1988) Charakterisierung und Identifizierung von Ektomykorrhizen in einem Fichtenbestand mit Untersuchungen zur Merkmalsvariabilität in sauer beregneten Flächen. — Bibliotheca Mycologica 125: 1–216.

    Google Scholar 

  • Gronbach E, Agerer R (1986) Charakterisierung und Inventur der Fichten-Mykorrhizen im Höglwald und deren Reaktion auf saure Beregnung. — Forstwissenschaftliches Centralblatt 105: 329–335.

    Google Scholar 

  • Haug I (1989) Intercellular infection in the meristematic region of Piceirhiza gelatinosa mycorrhizas. — New Phytologist 111: 203–207.

    Google Scholar 

  • Haug I, Pritsch K (1992) Ectomycorrhizal types of spruce (Picea abies (L.)Karst.) in the Black Forest. Kernforschungszentrum Karlsruhe, 87pp.

    Google Scholar 

  • Haug I, Pritsch K, Oberwinkler F (1992) Der Einfluß von Düngung auf Feinwurzeln und Mykorrhizen im Kulturversuch und im Freilamd. KfK-PEF (Karlsruhe) 97: 1–159.

    Google Scholar 

  • Henrion B, Chevalier G, Martin F (1994) Typing truffle species by PCR amplification of ribosomal DNA spacers. Mycological Research 98: 37–43.

    CAS  Google Scholar 

  • Horton T (2000) http://mendel.berkeley.edu/boletus.html (ML56 database)

  • Horton TR, Bruns TD (1998) Multiple-host fungi are the most frequent and abundant ectomycorrhizal types in a mixed stand of Douglas fir (Pseudotsuga menziesii) and bishop pine (Pinus muricata). — New Phytologist 139: 331–339.

    Article  Google Scholar 

  • Jonsson L, Dahlberg A, Nilsson M-C, Zackrisson O, Karen O (1999a) Ectomycorrhizal fungal communities in late-successional Swedish boreal forests, and their composition following wild-fire. — Molecular Ecology 8: 205–215.

    Article  Google Scholar 

  • Jonsson T, Kokalj S, Finlay R, Erland S (1999b) Ectomycorrhizal community structure in a limed spruce forest. — Mycological Research 103: 501–508.

    Article  Google Scholar 

  • Karen O, Högberg N, Dahlberg A, Jonsson L, Nylund JE (1997) Inter-and intraspecific variation in the ITS-region of rDNA of ectomycorrhizal fungi in Fennoscandia as detected endonuclease analysis. — New Phytologist 136: 313–325.

    Article  CAS  Google Scholar 

  • Karen O, Nylund J-E (1997) Effects of ammonium sulphate on the community structure and biomass of ectomycorrhizal fungi in a Norway spruce stand in southwestern Sweden. — Canadian Journal of Botany 75: 1628–1642.

    CAS  Google Scholar 

  • Kernaghan G (2001) Ectomycorrhizal fungi at tree line in the Canadian Rockies. — Mycorrhiza 10: 217–229.

    Article  CAS  Google Scholar 

  • Mahmood S, Finlay RD, Erland S (1999) Effects of repeated harvesting of forest residues on the ectomycorrhizal community in a Swedish spruce forest. — New Phytologist 142: 577–585.

    Article  Google Scholar 

  • Mleczko P (1998) Pinirhiza globulifera + Pinus sylvestris. In: Agerer R, Danielson RM, Egli S, Ingleby K, Luoma D, Treu R (eds) Descriptions of Ectomycorrhizae 3: 61–66.

  • Nylund JE, Dahlberg A, Högberg N, Karen OGK, Jonsson L (1995) Methods for studying species composition of mycorrhizal fungal communities in ecological studies and environmental monitoring. In: Stocchi V (ed) Biotechnology of ectomycorrhizae. Plenum Press, New York, pp 229–239.

    Google Scholar 

  • O’Donnell K (1993) Fusarium and its near relatives. In: Reynolds DR, Taylor JW (eds) The Fungal Holomorph: mitotic, meiotic and pleomorphic speciation in fungal systematics. CAB International, Washington, pp 225–233.

    Google Scholar 

  • Pritsch K, Boyle H, Munch JC, Buscot F (1997) Characterization and identification of black alder ectomycorrhizas by PCR/RFLP analyses of the rDNA internal transcribed spacer (ITS). — New Phytologist 137: 357–369.

    Article  CAS  Google Scholar 

  • Raspe S, Feger KH, Zöttl HW (eds) (1998) Ökosystemforschung im Schwarzwald. Auswirrkungen von atmogenen Einträgen und Restabilisierungsmasßnahmen auf den Wasser-und Stoffhaushalt von Fichtenwäldern. Verbundprojekt ARINUS. Ecomed-Verlag, Landsberg, 533 pp.

  • Saitou N, Nei M (1987) The Neighbor-joining Method: A new method for reconstructing phylogenetic trees. — Molecular Biology and Evolution B: 406–425.

  • Taylor DL, Bruns TD (1997) Independend, specialized invasions of ectomycorrhizal mutualism by two nonphotosynthetic orchids. — Proceedings of the National Academy of Science of the USA 94: 4510–4515.

    CAS  Google Scholar 

  • Taylor DL, Bruns TD (1999) Community structure of ectomycorrhizal fungi in a Pinus muricata forest: minimal overlap between the mature forest and resistant propagule communities. — Molecular Ecology 8: 1837–1850.

    CAS  PubMed  Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) ClustalW: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. — Nucleic Acids Research 22: 4673–4680.

    CAS  PubMed  Google Scholar 

  • Uhl M (1988) Identifizierung und Charakterisierung von Ektomykorrhizen an Pinus sylvestris und Ektomykorrhizen aus der Gattung Tricholoma. Dissertation München.

  • Vesterhold J (1995) Hebeloma crustuliniforme and related taxa: notes on some characters of taxonomic importance. — Symbolae Botanicae Upsalienses 30: 129–137.

    Google Scholar 

  • Vralstad T, Fossheim T, Schumacher T (2000) Piceirhiza bicolorata — the ectomycorrhizal expression of the Hymenoscyphus ericae aggregate? — New Phytologist 145: 549–563.

    Article  CAS  Google Scholar 

  • Weiß M, Oberwinkler F (2001) Phylogenetic relationships in Auriculariales and related groups — hypotheses derived from nuclear ribosomal DNA sequences. — Mycological Research 105: 403–415.

    Article  Google Scholar 

  • Weiß M, Yang ZL, Oberwinkler F (1998) Molecular phylogenetic studies in the genus Amanita. — Canadian Journal of Botany 76: 1170–1179.

    Article  Google Scholar 

  • White TJ, Bruns TD, Lee SB, Taylor JW (1990) Analysis of phylogenetic relationships by amplification and direct sequencing of ribosomal RNA genes. In: Innis MA, Gelfand DH, Sninsky JN, White TJ (eds) PCR protocols: a guide to methods and applications. Academic Press, New York, pp 315–322.

    Google Scholar 

  • Zak B (1971) Characterization and identification of Douglas fir mycorrhizae. In: Hacskaylo E (ed) Proc. 1st NACOM, US Government Printing Office, Washington DC, pp 38–53.

    Google Scholar 

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  1. Institut für Botanik, Spezielle Botanik & Mykologie, Universität Tübingen, Auf der Morgenstelle 1, D-72076, Tübingen, Germany

    Ingeborg Haug

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  1. Ingeborg Haug
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Haug, I. Identification of Picea-ectomycorrhizae by comparing DNA-sequences. Mycol Progress 1, 167–178 (2002). https://doi.org/10.1007/s11557-006-0016-9

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