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Cutting Efficiency of a Newly Developed Incubator-Based Laser Cutting Method and Its Effects on Potato Tissue Culture Plantlet Growth

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Abstract

Currently, potato tissue culture plantlets are often cut by mechanical shear in the process of transplanting. However, this method requires the repeated disinfection of cutting tools, which is not conducive to the realisation of mechanised operations. To solve this problem, a laser cutting method of potato tissue culture plantlets was explored based on our newly developed incubator in this study. The laser cutting experiments of the potato tissue culture plantlets were performed by two methods (row-by-row cutting and column-by-column cutting). The experimental results indicated that the efficiency of row-by-row cutting of potato tissue culture plantlets was higher than that of column-by-column cutting. When the optical output power of CO2 laser was 70 W and the cutting speed was 36 mm/s, it only took 8.3 s to complete the cutting of the whole box of tissue culture plantlets. In addition, the biological activity experiments of potato tissue culture plantlets indicated that all the growth indices (including survival rate, plant height, internode number, internode length, stem diameter, root length, and rootless fresh weight) of tissue culture plantlets through row-by-row cutting were superior to those through mechanical cutting. Our experiment shows that contactless cutting of tissue culture plantlets, high cutting efficiency, and no pollution can be achieved through the new culture incubator in combination with laser cutting technology. Our findings provide reference for the development of novel potato cutting transplantation equipment.

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Funding

This study was supported (project no. CARS-09-P08) by the China Potato Modern Agricultural Technology System Project (project no. HBHZD-ZB-2020–005-08) and by China Agriculture Research System of Hubei.

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Authors and Affiliations

  1. College of Engineering, Huazhong Agricultural University, Wuhan, 430070, China

    Xu Tao, Hongbing Duan, Rui Yang, Feihu Yao, Benfang Tan & Fengshun Liu

  2. Key Laboratory of Potato Biotechnology, Ministry of Agriculture and Rural Affairs, Wuhan, 430070, China

    Hongbing Duan & Xingkui Cai

Authors
  1. Xu Tao
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  2. Hongbing Duan
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  3. Xingkui Cai
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  4. Rui Yang
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  5. Feihu Yao
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  6. Benfang Tan
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  7. Fengshun Liu
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Contributions

XT contributed to the conception, design, methodology, data collection, data curation, interpretation, analysis, drafting, and revision of the paper. DHB contributed to the conception, design, supervision, and funding acquisition of the paper. CXK and YR contributed to the study’s conception and methodology. YFH, TBF, and LFS contributed to the data collection of the study. All authors approved the final version of the manuscript and agree to be accountable for all aspects of the work.

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Correspondence to Hongbing Duan.

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Xu, T., Duan, H., Cai, X. et al. Cutting Efficiency of a Newly Developed Incubator-Based Laser Cutting Method and Its Effects on Potato Tissue Culture Plantlet Growth. Potato Res. 66, 889–903 (2023). https://doi.org/10.1007/s11540-022-09600-w

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