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The Biopotential of a Wood-decaying Strain of Trichoderma harzianum for Protecting and Promoting the Sensitive Early Roots of Micropropagated Potato Plantlets and Reducing Transplantation Stress in an Aeroponic System

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Abstract

Minituber production in aeroponics is a growing trend in the potato seed industry for ensuring high-quality disease-free seed, as it requires vigorous, healthy plantlets to survive under stressful conditions. Fungi can assist plantlets endure stress, and there is a pressing need to explore various native fungi from an environmentally beneficial standpoint. In this study, three strains of Trichoderma harzianum were tested for their ability to protect the early roots of tissue culture–raised potato plantlets during hardening and to reduce transplantation stress in an aeroponic system. In the bioactivity results, strain TI21 isolated from decayed wood significantly improved root length by 42.7%, shoot length by 25.9% and biomass weight by 29.9% of tomato seedlings compared to control and demonstrated strong biocontrol activity (percentage) against four phytopathogens, including Sclerotium delphinii SCR5 (75.97 ± 8.06), Fusarium equiseti PTR3 (67.27 ± 5.11), Curvularia spicifera BLR4 (61.66 ± 2.98) and Alternaria alternata ALT10 (61.90 ± 4.58). Strains TI26 and TI27, isolated from rice rhizospheric soil, yielded no significant results. In potato-plantlet-hardening experiments, TI21 increased plantlet survival by 22.46%, 25.75% and 24.12%, compared to TI26, TI27, and control, respectively, by reducing root-rot caused by Sclerotium sp. and Fusarium sp. After 7 days of transplantation in aeroponic, TI21-treated plantlets had a significantly higher survival (90.5%) than control (77.6%), but there were no significant differences after 12 days, revealing that plantlets were much more susceptible to transplantation stress within the first 7 days of transplantation, which was overcome by TI21-treated plantlets. According to the findings, strain TI21 can be employed in micropropagated plantlets’ hardening to improve survival rates in aeroponic systems.

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Data Availability

The datasets generated during and/or analysed during the current study are available from the corresponding author on reasonable request.

References

  • Abdellaoui R, Boughalleb F, Zayoud D, Neffati M, Bakhshandeh E (2019) Quantification of Retama raetam seed germination response to temperature and water potential using hydrothermal time concept. Environs Exp Bot 157:211–216. https://doi.org/10.1016/j.envexpbot.2018.10.014

    Article  Google Scholar 

  • Abuhena M, Al-Rashid J, Azim MF, Khan MNM, Kabir MG, Barman NC, Rasul NM (2022) Optimization of industrial (3000L) production of Bacillus subtilis CW-S and its novel application for minituber and industrial-grade potato cultivation. Sci Rep 12:11153. https://doi.org/10.1038/s41598-022-15366-5

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Abuhena M, Kabir MG, Azim MA, Al-Rashid J, Rasul NM, Huq MA (2022) A stressing method for producing high-density Trichoderma spores in a dual-layer by utilizing a starch-based medium in a reconditioning approach. Bioresour Technol Rep 19:101165. https://doi.org/10.1016/j.biteb.2022.101165

    Article  CAS  Google Scholar 

  • Ahemad M, Kibret M (2014) Mechanisms and applications of plant growth promoting rhizobacteria: current perspective. J King Saud Univ Sci 26(1):1–20. https://doi.org/10.1016/j.jksus.2013.05.001

    Article  Google Scholar 

  • Ahmad P, Hashem A, Abd-Allah EF, Alqarawi A, John R, Egamberdieva D, Gucel S (2015) Role of Trichoderma harzianum in mitigating NaCl stress in Indian mustard (Brassica juncea L) through antioxidative defense system. Front Plant Sci 6:868. https://doi.org/10.3389/fpls.2015.00868

  • Al-Taleb M, Hassawi D, Abu-Romman S (2011) Production of virus free potato plants using meristem culture from cultivars grown under Jordanian environment. Am- Eurasian j Agric Environ Sci 11:467–472

    CAS  Google Scholar 

  • Araújo GC, Sousa NR, Castro PM (2018) The effect of fungal-bacterial interaction on the phenolic profile of Pinus pinea L. Plant Growth Regul 86:465–475

    Article  Google Scholar 

  • Atashi S, Bakhshandeh E, Mehdipour M, Jamali M, Teixeira Da Silva JA (2015) Application of a hydrothermal time seed germination model using the Weibull distribution to describe base water potential in zucchini (Cucurbita pepo L.). J Plant Growth Regul 34:150–157. https://doi.org/10.1007/s00344-014-9452-y

    Article  CAS  Google Scholar 

  • Azad M, Khatun Z, Eaton T, Hossen M, Soren HM, E, (2020) Generation of virus free potato plantlets through Meristem culture and their field evaluation. Am J Plant Sci 11:1827–1846. https://doi.org/10.4236/ajps.2020.1111131

    Article  CAS  Google Scholar 

  • Bakhshandeh E, Pirdashti H, Gilani Z (2018) Application of mathematical models to describe rice growth and nutrients uptake in the presence of plant growth promoting microorganisms. Appl Soil Ecol 124:171–184

    Article  Google Scholar 

  • Bakhshandeh E, Pirdashti H, Vahabinia F, Gholamhossieni F (2020) Quantification of the effect of environmental factors on seed germination and seedling growth of Eruca (Eruca sativa) using mathematical models. J. Plant Growth Regul. 39:190–204. https://link.springer.com/article/https://doi.org/10.1007/s00344-019-09974-1

  • Bano A, Yasmeen S (2010) Role of phytohormones under induced drought stress in wheat. Pak J Bot 42:2579–2587

    CAS  Google Scholar 

  • BBS (2017) Yearbook of agricultural statistics (ed. SID). Ministry of Planning, pp. 30–595.

  • Benítez T, Rincón AM, Limón MC, Codón AC (2004) Biocontrol mechanism of Trichoderma strains. Int Microbiol 7:249–260

  • Bianco C, Defez R (2009) Medicago truncatula improves salt tolerance when nodulated by an indole-3-acetic acid-overproducing Sinorhizobium meliloti strain. J Exp Bot 60:3097–3107

    Article  PubMed  CAS  Google Scholar 

  • Calori A, Factor T, Feltran J, Watanabe E, Moraes C, Purquerio LF (2018) Seed potato minituber production in aeroponic system under tropical conditions (fall/winter): electrical conductivity and plant density. J Plant Nutr 41:2200–2209. https://doi.org/10.1080/01904167.2018.1497652

    Article  CAS  Google Scholar 

  • Chandra S, Bandopadhyay R, Kumar V (2010) Acclimatization of tissue cultured plantlets: from laboratory to land. Biotechnol Lett 32:1199–1205

    Article  PubMed  CAS  Google Scholar 

  • Desai S, Reddy MS, Kloepper JW, Gnanamanickam SS (2002) Biological control of crop diseases. Marcel Dekker Inc., Comprehensive testing of biological agents, New York, USA, pp 387–420

    Google Scholar 

  • Egamberdieva D, Jabborova D, Berg G (2016) Synergistic interactions between Bradyrhizobium japonicum and the endophyte Stenotrophomonas rhizophila and their effects on growth, and nodulation of soybean under salt stress. Plant Soil 405:35–45. https://doi.org/10.1007/s11104-015-2661-8

    Article  CAS  Google Scholar 

  • El-Naggar M, Kövics GJ, Sánd E, Irinyi L, (2008) Mycoparasitism and antagonistic efficiency of Trichoderma reesei against Botrytis spp. Contrib Bot 43:141–147

  • Fiske CH, Subbarow YJ (1925) The colorimetric determination of phosphorus. Biol Chem 66:375–400

    Article  CAS  Google Scholar 

  • Gordon SA, Weber RP (1951) Colorimetric estimation of indoleacetic acid. Plant Physiol 26:192–195

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Habib SH, Kausar H, Saud HM (2016) Plant growth-promoting rhizobacteria enhance salinity stress tolerance in okra through ROS-scavenging enzymes. BioMed Res Inter 62:845–847. https://doi.org/10.1155/2016/6284547

    Article  CAS  Google Scholar 

  • Ilangumaran G, Smith DL (2017) Plant growth promoting rhizobacteria in amelioration of salinity stress: a systems biology perspective. Front Plant Sci 8:1768. https://doi.org/10.3389/fpls.2017.01768

    Article  PubMed  PubMed Central  Google Scholar 

  • Jahan M, Abuhena M, Azad AK, Karim MM (2018) In vitro antibacterial and antibiofilm activity of selected medicinal plants and spices extracts against multidrug resistant Pseudomonas aeruginosa. J Pharmacogn Phytochem 7(3):2114–2121

    CAS  Google Scholar 

  • Khademian R, Asghari B, Sedaghati B, Yaghoubian Y (2019) Plant beneficial rhizospheric microorganisms (PBRMs) mitigate deleterious effects of salinity in sesame (Sesamum indicum L.): physiobiochemical properties, fatty acids composition and secondary metabolites content. Ind Crops Prod 136:129–139. https://doi.org/10.1016/j.indcrop.2019.05.002

    Article  CAS  Google Scholar 

  • Kiani A, Nami Y, Hedayati S, Mehdi J, Hadi S, Babak H (2021) Tarkhineh as a new microencapsulation matrix improves the quality and sensory characteristics of probiotic Lactococcus lactis KUMS-T18 enriched potato chips. Sci Rep 11:12599. https://doi.org/10.1038/s41598-021-92095-1

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Lucy M, Reed E, Glick BR (2004) Applications of free living plant growth-promoting rhizobacteria. Antonie Van Leeuwenhoek 86:1–25

    Article  PubMed  CAS  Google Scholar 

  • Maitra P, Al-Rashid J, Barman NC, Khan MNM, Mandal D, Rasul NM et al (2021) sand particle size and phosphorus amount affect Rhizophagus irregularis spore production using in vitro propagated spore as a starter inoculum in rhizosphere of maize (Zea mays) Plantlets. J Fungi (basel) 7:846. https://doi.org/10.3390/jof7100846

    Article  PubMed  CAS  Google Scholar 

  • Maral Gezgin Y, Sozer Gül D, Kara Sensatar S, Sargın CU, Sukan S, Eltem FV, R, (2020) Evaluation of Trichoderma atroviride and Trichoderma citrinoviride growth profiles and their potentials as biocontrol agent and biofertilizer. Turkish J Biochem 45(2):163–175. https://doi.org/10.1515/tjb-2018-0378

    Article  CAS  Google Scholar 

  • Mbiyu MW, Lung’aho C, Otieno SA, Nyongesa MW, Muchui MN, Ogemma JN, (2018) Performance of five potato varieties with regards to growth and production of mini-tubers under an aeroponic system in central highlands of Kenya. Afr J Agric Res 13:366–378. https://doi.org/10.5897/AJAR2017.12762

    Article  CAS  Google Scholar 

  • Meena VS, Maurya BR, Verma JP, Aeron A, Kumar A, Kim K, Bajpai VK (2015) Potassium solubilizing rhizobacteria (KSR): isolation, identification, and K-release dynamics from waste mica. Ecol Eng 81:340–347. https://doi.org/10.1016/j.ecoleng.2015.04.065

    Article  Google Scholar 

  • Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar Anal. Chem. pp. 426–428.

  • Mishra J, Singh R, Arora NK (2017) Plant growth-promoting microbes: diverse roles in agriculture and environmental sustainability. Probiotics and plant health. Springer, Singapore, pp. 71–111.

  • Mohammadi Kashka F, Pirdashti H, Yaghoubian Y, Bahari Saravi SH (2016) Effect of Trichoderma virens and Piriformospora indica coexistence with Enterobacter sp. on the growth and photosynthetic pigments of pepper (Capsicum annuum L.) plant. Plant Ecophysiol 8:121–133

    Google Scholar 

  • Muthuraj R, Singh B, Buckseth T, Singh R, Singh S, Sharma A (2016) Effect of micro-plants hardening on aeroponic potato seed production. Potato Journal 43:214–219

    Google Scholar 

  • Nautiyal CS (1999) An efficient microbiological growth medium for screening phosphate solubilizing microorganisms. FEMS. pp. 265–270.

  • Niu X, Song L, Xiao Y, Ge W (2018) Drought-tolerant plant growth promoting rhizobacteria associated with foxtail millet in a semiarid agro ecosystem and their potential in alleviating drought stress. Front Microbiol 8:2580. https://doi.org/10.3389/fmicb.2017.02580

    Article  PubMed  PubMed Central  Google Scholar 

  • Patel RR, Patel DD, Thakor P, Patel B, Thakkar VR (2015) Alleviation of salt stress in germination of Vigna radiata L. by two halotolerant Bacilli sp. isolated from saline habitats of Gujarat. Plant Growth Regul 76:51–60. https://doi.org/10.1007/s10725-014-0008-8

    Article  CAS  Google Scholar 

  • Patten CL, Glick BR (1996) Bacterial biosynthesis of indole-3-acetic acid. Can J Microbiol 42:207–220

    Article  PubMed  CAS  Google Scholar 

  • Rai S, Kashyap PL, Kumar S, Srivastava AK, Ramteke PW (2016) Identification, characterization and phylogenetic analysis of antifungal Trichoderma from tomato rhizosphere. SpringerPlus 5:1939. 10.1186%2Fs40064–016–3657–4

  • Rilling J, Acuña J, Nannipieri P, Cassan F, Maruyama F, Jorquera M (2019) Current opinion and perspectives on the methods for tracking and monitoring plant growth-promoting bacteria. Soil Biol Biochem 130:205–219. https://doi.org/10.1016/j.soilbio.2018.12.012

    Article  CAS  Google Scholar 

  • Sabur SA, Monayem Miah MA, Esmat Ara Begum M, Shahrukh Rahman M, Uddin Molla MM, Alam L (2021) Demand, supply and reasons for price hike of potato in Bangladesh. The Bangladesh Journal of Agricultural Economics 42:93–108

    Google Scholar 

  • Saikia J, Sarma RK, Dhandia R, Yadav A, Bharali R, Gupta VK, Saikia R (2018) Alleviation of drought stress in pulse crops with ACC deaminase producing rhizobacteria isolated from acidic soil of Northeast India. Sci Rep 8:3560. https://doi.org/10.1038/s41598-018-21921-w

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Sharma K, Dak G, Agrawal A, Bhatnagar M, Sharma R (2007) Effect of phosphate solubilizing bacteria on the germination of Cicer arietinum seeds and seedling growth. J Herbal Medic Toxicol 1:61–63

  • Shoresh M, Harman GE, Mastouri F (2010) Induced systemic resistance and plant responses to fungal biocontrol agents. Annu Rev Phytopathol 48:21–43. https://doi.org/10.1146/annurev-phyto-073009-114450

    Article  PubMed  CAS  Google Scholar 

  • Siddique MNA, Sultana J, Huda MS, Abdullah MR, Chowdury M (2015) Potato production and management with preference to seed potato supply chain, certification and actors involve in Bangladesh. International Journal of Business, Management and Social Research 01:01–13

    Article  Google Scholar 

  • Silva Filho JB, Fontes PCR, Cecon PR, McGiffen MEJr, (2018) Evaluation of “UFV aeroponic system” to produce basic potato seed minitubers. Amer J Potato Res 95:443–450

    Article  Google Scholar 

  • Singh S, Singh V, Pandey SK (2010) Aeroponic for potato seed production. ICAR News A Sci Technol News 16:1–2

    Google Scholar 

  • Tamura K, Nei M (1993) Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10:512–526. https://doi.org/10.1093/oxfordjournals.molbev.a040023

    Article  PubMed  CAS  Google Scholar 

  • Tiwari S, Prasad V, Chauhan PS, Lata C (2017) Bacillus amyloliquefaciens confers tolerance to various abiotic stresses and modulates plant response to phytohormones through osmoprotection and gene expression regulation in rice. Front Plant Sci 8:1510. https://doi.org/10.3389/fpls.2017.01510

    Article  PubMed  PubMed Central  Google Scholar 

  • Vahabinia F, Pirdashti H, Bakhshande E (2019) Environmental factors’ effect on seed germination and seedling growth of chicory (Cichorium intybus L) as an important medicinal plant. Acta Physiol Planta 41:27. https://doi.org/10.1007/s11738-019-2820-2

    Article  CAS  Google Scholar 

  • Wardle K, Quinland A, Short KC (1983) In vitro acclimatization of aseptically cultured plantlets to humidity. J Amer Soc Hort Sci 108:386–389

    Article  Google Scholar 

  • Xue M, Wang R, Zhang C, Wang W, Zhang F, Chen D (2021) Screening and identification of Trichoderma strains isolated from natural habitats in China with potential agricultural applications. Biomed Res Int 2021:7913950. https://doi.org/10.1155/2021/7913950

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  • Yadav J, Verma JP, Jaiswal DK, Kumar A (2014) Evaluation of PGPR and different concentration of phosphorus level on plant growth, yield and nutrient content of rice (Oryza sativa). Ecol Eng 62:123–128

    Article  Google Scholar 

  • Yaghoubi KM, Pirdashti H, Rahimian H, Nematzadeh G, Sepanlou MG (2018) Potassium solubilising bacteria (KSB) isolated from rice paddy soil: from isolation, identification to K use efficiency. Symbiosis 76:13–23. https://doi.org/10.1007/s13199-017-0533-0

    Article  CAS  Google Scholar 

  • Yedidia I, Srivastva AK, Chet KY, I, (2001) Effect of Trichoderma harzianum on microelement concentrations and increased growth of cucumber plants. Plant Soil 235:235–242. https://doi.org/10.1023/A:1011990013955

    Article  CAS  Google Scholar 

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Funding

This study was supported by Apex Biofertilizers and Biopesticides Limited, Go-bindaganj-5740, Gaibandha, Bangladesh and Apex Biotechnology laboratory, Apex Holdings Ltd., East Chandora, Shafipur, Kaliakoir, Gazipur 1751, Bangladesh.

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  1. Abuhena, Golam Kabir and Faisal Azim contributed equally to this work.

Authors and Affiliations

  1. Department of Research & Development, Apex Biofertilizers & Biopesticides Limited, Gobindaganj-5740, Gaibandha, Bangladesh

    Md. Abuhena, Md. Golam Kabir, Md. Faisal Azim, Jubair Al-Rashid & Noorain Munim Rasul

  2. Apex Biotechnology Laboratory, Apex Holdings Ltd, East Chandora, Shafipur, Kaliakoir, Gazipur, 1751, Bangladesh

    Md. Golam Kabir, Mohammad Sharif Sarker, Jubair Al-Rashid, Md. Niuz Morshed Khan, Nirmal Chandra Barman & Noorain Munim Rasul

  3. Department of Food Science and Biotechnology, College of BioNano Technology, Gachon University, Seongnam, 461-701, Republic of Korea

    Shahina Akter

  4. Department of Food and Nutrition, College of Biotechnology and Natural Resource, Chung-Ang University, Anseong, Gyeonggi-do, 17546, Republic of Korea

    Md. Amdadul Huq

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  1. Md. Abuhena
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Contributions

M.A., M.G.K. and M.F.A: conceptualization of the research work, data collection, performer of the experimental research work, statistical analysis, manuscript writing, manuscript revision and editing. M.S.S J.A.-R. M.N.M.K. and N.C.B.: performer of the experimental research work, reviewing, editing. N.M.R. and S.A.: manuscript writing, reviewing, editing and, approval of the protocol. M.A.H.: manuscript writing, reviewing, editing and submission of the manuscript. All authors have read and agreed to the published version of the manuscript.

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Correspondence to Md. Golam Kabir, Shahina Akter or Md. Amdadul Huq.

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Abuhena, M., Kabir, M.G., Azim, M.F. et al. The Biopotential of a Wood-decaying Strain of Trichoderma harzianum for Protecting and Promoting the Sensitive Early Roots of Micropropagated Potato Plantlets and Reducing Transplantation Stress in an Aeroponic System. Potato Res. 66, 663–681 (2023). https://doi.org/10.1007/s11540-022-09594-5

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