Evaluation of Genotoxic and Apoptotic Effects of Sprouted Potato

Sancer, Okan; Şahin, Uğur; Ateş, Meryem; Yünlü, Sibel

doi:10.1007/s11540-022-09560-1

Published: 13 June 2022

Evaluation of Genotoxic and Apoptotic Effects of Sprouted Potato

Potato Research (2022)Cite this article

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Abstract

Potato is among the foods we consume frequently in our daily life. The harmful effects of consuming sprouted potatoes on our health are mentioned. Sprouted potato contains higher levels of glycoalkaloids, which can have toxic effects in humans and animals. Discarding the sprouts, skin and green parts from tubers may help reduce risk of toxicity. In this study, we aimed to determine whether the flesh part of the sprouted potato has genotoxic and apoptotic effects. Phenolic compounds were determined by HPLC method in sprouted and unsprouted potato extracts. The genotoxicity and apoptosis effects of sprouted and unsprouted potato extracts at various concentrations were investigated in human peripheral lymphocytes by comet assay and DNA ladder assay. The phenolic compounds of chlorogenic acid, vanilin, caffeic acid and o-coumaric acid were detected at a higher level in the sprouted potato than in the unsprouted potato, while only p-hydroxybenzoic acid was determined at a lower level. As a result of our research, we detect that the sprouted potato extract did not cause statistically significant DNA damage in human lymphocytes (p > 0.05), and we could not detect DNA fragment images of human peripheral lymphocytes in the DNA ladder assay. It was concluded that consuming sprouted potatoes (flesh part) by discarding the sprouts, eyes, green skin, and bruised parts may help reduce the glycoalkaloid-induced genotoxicity and apoptosis.

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Acknowledgements

The authors acknowledge the members of the Innovative Technologies Application and Research Center of Suleyman Demirel University, the Medical Biology of Suleyman Demirel University Faculty of Medicine for their support in preparing this work and for making it possible.

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Authors and Affiliations

Department of Medical Biology, Faculty of Medicine, University of Süleyman Demirel, Isparta, Turkey
Okan Sancer
Genetic Research Unit, Innovative Technologies Application and Research Center, University of Süleyman Demirel, Isparta, Turkey
Okan Sancer, Uğur Şahin & Meryem Ateş
Department of Chemistry, Faculty of Arts and Sciences, University of Süleyman Demirel, Isparta, Turkey
Uğur Şahin & Sibel Yünlü
Department of Plant Protection, Faculty of Agriculture, Isparta University of Applied Sciences, Isparta, Turkey
Meryem Ateş
Applied Basic Sciences Unit, Innovative Technologies Application and Research Center, University of Süleyman Demirel, Isparta, Turkey
Sibel Yünlü

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Okan Sancer
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Uğur Şahin
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Meryem Ateş
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Sibel Yünlü
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All the authors participated in this study including data interpretation and manuscript preparation. All the authors took full responsibility for the manuscript.

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Correspondence to Okan Sancer.

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This study was approved by Isparta Süleyman Demirel University Medical Faculty Ethics Committee (No: 7/90).

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Sancer, O., Şahin, U., Ateş, M. et al. Evaluation of Genotoxic and Apoptotic Effects of Sprouted Potato. Potato Res. (2022). https://doi.org/10.1007/s11540-022-09560-1

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Received: 30 March 2022
Accepted: 31 May 2022
Published: 13 June 2022
DOI: https://doi.org/10.1007/s11540-022-09560-1

Keywords

Apoptosis
Comet assay
Phenolic compounds
Potato