Abstract
Potato is among the foods we consume frequently in our daily life. The harmful effects of consuming sprouted potatoes on our health are mentioned. Sprouted potato contains higher levels of glycoalkaloids, which can have toxic effects in humans and animals. Discarding the sprouts, skin and green parts from tubers may help reduce risk of toxicity. In this study, we aimed to determine whether the flesh part of the sprouted potato has genotoxic and apoptotic effects. Phenolic compounds were determined by HPLC method in sprouted and unsprouted potato extracts. The genotoxicity and apoptosis effects of sprouted and unsprouted potato extracts at various concentrations were investigated in human peripheral lymphocytes by comet assay and DNA ladder assay. The phenolic compounds of chlorogenic acid, vanilin, caffeic acid and o-coumaric acid were detected at a higher level in the sprouted potato than in the unsprouted potato, while only p-hydroxybenzoic acid was determined at a lower level. As a result of our research, we detect that the sprouted potato extract did not cause statistically significant DNA damage in human lymphocytes (p > 0.05), and we could not detect DNA fragment images of human peripheral lymphocytes in the DNA ladder assay. It was concluded that consuming sprouted potatoes (flesh part) by discarding the sprouts, eyes, green skin, and bruised parts may help reduce the glycoalkaloid-induced genotoxicity and apoptosis.
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References
Akyol H, Riciputi Y, Capanoglu E, Caboni MF, Verardo V (2016) Phenolic compounds in the potato and its byproducts: an overview. Int J Mol Sci 17(6):835. https://doi.org/10.3390/ijms17060835
Albishi T, John JA, Al-Khalifa AS, Shahidi F (2013) Antioxidant, anti-inflammatory and DNA scission inhibitory activities of phenolic compounds in selected onion and potato varieties. J Funct Foods 5(2):930–939. https://doi.org/10.1016/j.jff.2013.02.005
Arvas YE, Aksoy HM, Yılmaz K (2018) Patates bitkisinde biyoteknolojik çalışmalar. Int J Life Sci Biotechnol 1(1):37–47
Bacanlı M, Aydın S, Taner G, Şahin T, Başaran A, Başaran N (2013) Antigenotoxic effects of ferulic acid on sepsis-induced DNA damage in the liver and kidney of Wistar albino rats. Toxicol Lett 221:S125. https://doi.org/10.1016/j.toxlet.2013.05.226
Brown, C. (2005) Antioxidants in potato. American journal of potato research 82(2):163–172. https://doi.org/10.1007/BF02853654
Caponio F, Alloggio V, Gomes T (1999) Phenolic compounds of virgin olive oil: influence of paste preparation techniques. Food Chem 64(2):203–209. https://doi.org/10.1016/S0308-8146(98)00146-0
Çavdar, C., Sİfİl, A, Çamsarı, T. (1997) Reaktif oksijen partikulleri ve antioksidan savunma. Türk Nefroloji, Diyaliz ve Transplantasyon Dergisi 6(3-4)
Chen S, Yin Q, Hu H, Chen Q, Huang Q, Zhong M (2021) AOPPs induce HTR-8/SVneo cell apoptosis by downregulating the Nrf-2/ARE/HO-1 anti-oxidative pathway: Potential implications for preeclampsia. Placenta 112:1–8. https://doi.org/10.1016/j.placenta.2021.06.008
Dale MFB, Griffiths DW, Bain H (1998) Effect of bruising on the total glycoalkaloid and chlorogenic acid content of potato (Solanum tuberosum) tubers of five cultivars. J Sci Food Agric 77(4):499–505. https://doi.org/10.1002/(SICI)1097-0010(199808)77:4<499::AID-JSFA72>3.0.CO;2-G
Friedman M (2006) Potato glycoalkaloids and metabolites: roles in the plant and in the diet. J Agric Food Chem 54(23):8655–8681. https://doi.org/10.1021/jf061471t
Friedman M, Henika P (1992) Absence of genotoxicity of potato alkaloids α-chaconine, α-solanine and solanidine in the Ames Salmonella and adult and foetal erythrocyte micronucleus assays. Food Chem Toxicol 30(8):689–694. https://doi.org/10.1016/0278-6915(92)90164-G
Friedman M, Henika P, Mackey B (1996) Feeding of potato, tomato and eggplant alkaloids affects food consumption and body and liver weights in mice. J Nutr 126(4):989–999. https://doi.org/10.1093/jn/126.4.989
Gao S-Y, Wang Q-J, Ji Y-B (2006) Effect of solanine on the membrane potential of mitochondria in HepG2 cells and [Ca2+] i in the cells. World J Gastroenterol: WJG 12(21):3359. https://doi.org/10.3748/wjg.v12.i21.3359
Hellenäs K-E, Nyman A, Slanina P, Lööf L, Gabrielsson J (1992) Determination of potato glycoalkaloids and their aglycone in blood serum by high-performance liquid chromatography: application to pharmacokinetic studies in humans. J Chromatogr B Biomed Sci Appl 573(1):69–78. https://doi.org/10.1016/0378-4347(92)80476-7
Hesam F, Balali GR, Tehrani RT (2012) Evaluation of antioxidant activity of three common potato (Solanum tuberosum) cultivars in Iran. Avicenna J Phytomed 2(2):79
Kolaç T, Gürbüz P, Yetiş G (2017) Doğal ürünlerin fenolik içeriği ve antioksidan özellikleri. İnönü Üniversitesi Sağlık Hizmetleri Meslek Yüksek Okulu Dergisi 5(1):26–42
Langkilde S, Mandimika T, Schrøder M, Meyer O, Slob W, Peijnenburg A, Poulsen M (2009) A 28-day repeat dose toxicity study of steroidal glycoalkaloids, α-solanine and α-chaconine in the Syrian Golden hamster. Food Chem Toxicol 47(6):1099–1108. https://doi.org/10.1016/j.fct.2009.01.045
Langkilde S, Schrøder M, Frank T, Shepherd LV, Conner S, Davies HV, Meyer O, Danier J, Rychlik M, Belknap WR (2012) Compositional and toxicological analysis of a GM potato line with reduced α-solanine content–a 90-day feeding study in the Syrian Golden hamster. Regul Toxicol Pharmacol 64(1):177–185. https://doi.org/10.1016/j.yrtph.2012.06.018
Lawen A (2003) Apoptosis—an introduction. Bioessays 25(9):888–896. https://doi.org/10.1002/bies.10329
Lee, K.-R., Kozukue, N., Han, J.-S., Park, J.-H., Chang, E.-y., Baek, E.-J., Chang, J.-S. and Friedman, M. (2004) Glycoalkaloids and metabolites inhibit the growth of human colon (HT29) and liver (HepG2) cancer cells. J Agric Food Chem 52(10), 2832-2839. doi: https://doi.org/10.1021/jf030526d
Lee SH, Oh SH, Hwang IG, Kim HY, Woo KS, Woo SH, Kim HS, Lee J, Jeong HS (2016) Antioxidant contents and antioxidant activities of white and colored potatoes (Solanum tuberosum L.). Prev Nutr Food Sci 21(2):110. https://doi.org/10.3746/pnf.2016.21.2.110
Nguyen TV, Do LTK, Somfai T, Otoi T, Taniguchi M, Kikuchi K (2019) Presence of chlorogenic acid during in vitro maturation protects porcine oocytes from the negative effects of heat stress. Anim Sci J 90(12):1530–1536. https://doi.org/10.1111/asj.13302
Ni W, Tian T, Zhang L, Li Z, Wang L, Ren A (2018) Maternal periconceptional consumption of sprouted potato and risks of neural tube defects and orofacial clefts. Nutr J 17(1):1–8. 2. https://doi.org/10.1186/s12937-018-0420-4
Owumi SE, Irozuru CE, Arunsi UO, Oyelere AK (2022) Caffeic acid protects against DNA damage, oxidative and inflammatory mediated toxicities, and upregulated caspases activation in the hepatorenal system of rats treated with aflatoxin B1. Toxicon 207:1–12. https://doi.org/10.1016/j.toxicon.2021.12.021
Özdemir P, Malayoğlu HB (2017) Patates işleme endüstrisi yan ürünleri ve hayvan beslemede değerlendirilmesi. Türk Tarım-Gıda Bilim ve Teknoloji dergisi 5(1):93–97
Phillips B, Hughes J, Phillips J, Walters D, Anderson D, Tahourdin C (1996) A study of the toxic hazard that might be associated with the consumption of green potato tops. Food Chem Toxicol 34(5):439–448. https://doi.org/10.19852/j.cnki.jtcm.20210209.008
Rayburn J, Bantle J, Qualls C Jr, Friedman M (1995) Protective effects of glucose-6-phosphate and NADP against α-chaconine-induced developmental toxicity in Xenopus embryos. Food Chem Toxicol 33(12):1021–1025. https://doi.org/10.1016/0278-6915(95)00080-1
Roddick JG, Rijnenberg AL (1987) Synergistic interaction between the potato glycoalkaloids α-solanine and α-chaconine in relation to lysis of phospholipid/sterol liposomes. Phytochemistry 26(5):1325–1328. https://doi.org/10.1016/S0031-9422(00)81804-X
Roddick JG, Rijnenberg AL, Osman SF (1988) Synergistic interaction between potato glycoalkaloidsα-solanine andα-chaconine in relation to destabilization of cell membranes: ecological implications. J Chem Ecol 14(3):889–902. https://doi.org/10.1007/BF01018781
Romanucci V, Pisanti A, Di Fabio G, Davinelli S, Scapagnini G, Guaragna A, Zarrelli A (2016) Toxin levels in different variety of potatoes: alarming contents of α-chaconine. Phytochem Lett 16:103–107. https://doi.org/10.1016/j.phytol.2016.03.013
Saraç MG, Hastaoğlu E, Dinçel B, Can ÖP (2021) Investigation of uses of purple and orange sweet potato dietary fiber concentrates in sucuks. Turk J Agric-Food Sci Technol 9(9):1679–1685. https://doi.org/10.24925/turjaf.v9i9.1679-1685.4650
Sevgi K, Tepe B, Sarikurkcu C (2015) Antioxidant and DNA damage protection potentials of selected phenolic acids. Food Chem Toxicol 77:12–21. https://doi.org/10.1016/j.fct.2014.12.006
Silva JP, Gomes AC, Coutinho OP (2008) Oxidative DNA damage protection and repair by polyphenolic compounds in PC12 cells. Eur J Pharmacol 601(1-3):50–60. https://doi.org/10.1016/j.ejphar.2008.10.046
Singh NP, McCoy MT, Tice RR, Schneider EL (1988) A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res 175(1):184–191. https://doi.org/10.1016/0014-4827(88)90265-0
Tınaztepe ÖE (2020) Sızma Zeytinyağı Fenoliklerinin İnsan Periferik Kan Mononükleer Hücrelerinde Oksidatif Mitokondriyal ve Nükleer DNA Hasarına Karşı Etkisi. J Adv Res Nat Appl Sci 6(2):231–238. https://doi.org/10.28979/jarnas.844774
Yang S-A, Paek S-H, Kozukue N, Lee K-R, Kim J-A (2006) α-Chaconine, a potato glycoalkaloid, induces apoptosis of HT-29 human colon cancer cells through caspase-3 activation and inhibition of ERK 1/2 phosphorylation. Food Chem Toxicol 44(6):839–846. https://doi.org/10.1016/j.fct.2005.11.007
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The authors acknowledge the members of the Innovative Technologies Application and Research Center of Suleyman Demirel University, the Medical Biology of Suleyman Demirel University Faculty of Medicine for their support in preparing this work and for making it possible.
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Sancer, O., Şahin, U., Ateş, M. et al. Evaluation of Genotoxic and Apoptotic Effects of Sprouted Potato. Potato Res. 65, 903–913 (2022). https://doi.org/10.1007/s11540-022-09560-1
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DOI: https://doi.org/10.1007/s11540-022-09560-1