Abstract
The prevalence of Fusarium dry rot in potatoes produced in Norway was investigated in a survey for three consecutive years in the period 2010 to 2012. A total of 238 samples (comprising 23,800 tubers) were collected, representing different cultivars and production regions in Norway. Fusarium spp. were detected in 47% of the samples, with one to three species per sample. In total, 718 isolates of Fusarium spp. were recovered and identified to seven species. The most commonly isolated species was Fusarium coeruleum, comprising 59.6% of the total Fusarium isolates and found in 17.2% of the collected samples, followed by Fusarium avenaceum (27.2% of the isolates and found in 27.7% of the samples). Fusarium sambucinum was the third most prevalent species (6.4% in 8.8% of the samples) and Fusarium culmorum the fourth (5.2% in 6.3% of the samples). Less prevalent species included Fusarium cerealis, Fusarium graminearum, and Fusarium equiseti (<1% in 0.4 to 1.3% of the samples). F. coeruleum was the most prevalent species in northern and southwestern Norway, whereas F. avenaceum was dominating in eastern Norway. The potato cultivars Berber and Rutt were susceptible to all Fusarium spp. A new TaqMan real-time PCR assay specific for F. coeruleum was developed, which successfully identified Norwegian isolates. This and other previously developed real-time PCR assays targeting different Fusarium species were evaluated for their ability to detect latent infections in potatoes at harvest. This study provides new information on the current occurrence of different Fusarium species causing Fusarium dry rot in potatoes in Europe including areas far into the arctic in the north of Norway.
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References
Abbas HK, Mirocha CJ, Berdal BP, Sundheim L, Gunther R, Johnsen B (1987) Isolation and toxicity of Fusarium species from various areas of Norway. Acta Agric Scand 37:427–435
Al-Mughrabi KI et al (2013) Efficacy of Pseudomonas syringae in the management of potato tuber diseases in storage. Biol Control 64:315–322
Bång U (1992) Influence of seed tuber infestation, chemical seed treatment, and pre-harvest climate on incidence of gangrene and dry rot of potato (Solanum tuberosum L.). Potato Res 35:3–15
Bernhoft A, Eriksen GS, Sundheim L, Berntssen M, Brantsæter AL, Brodal G, Fæste CK, Hofgaard IS, Rafoss T, Sivertsen T, Tronsmo AM (2013) Risk assessment of mycotoxins in cereal grain in Norway, Vitenskapskomiteen for mattrygghet (Norwegian Scientific Committee for Food Safety). www.vkm.no/dav/eee04d10c4.pdf. Accessed 01 May 2016
Bjor T (1978) Lagringsråter på potet -årsaker og forekomst, VI. Informasjonsmøte i plantevern Ås, 23.-24. januar 1978. pp. D1–D4
Bojanowski A, Avis TJ, Pelletier S, Tweddell RJ (2013) Management of potato dry rot. Postharvest Biol Technol 84:99–109
Boyd AEW (1972) Potato storage diseases Review of. Plant Pathol 51:305–308
Cullen DW, Lees AK, Toth IK, Duncan JM (2001) Conventional PCR and real-time quantitative PCR detection of Helminthosporium solani in Soil and on potato tubers. Eur J Plant Pathol 107:387–398
Cullen DW, Toth IK, Pitkin Y, Boonham N, Walsh I, Barker K, Lees AK (2005) Use of quantitative molecular diagnostic assays to investigate Fusarium dry rot in potato stocks and soil. Phytopathology 95:1462–1471
Du M, Ren X, Sun Q, Wang Y, Zhang R (2012) Characterization of Fusarium spp. causing potato dry rot in China and susceptibility evaluation of Chinese potato germplasm to the pathogen. Potato Res 55:175–184
Esfahani M (2005) Susceptibility assessment of potato cultivars to Fusarium dry rot species. Potato Res 48:215–226
Estrada R Jr, Gudmestad NC, Rivera VV, Secor GA (2010) Fusarium graminearum as a dry rot pathogen of potato in the USA: prevalence, comparison of host isolate aggressiveness and factors affecting aetiology. Plant Pathol 59:1114–1120
Gachango E, Hanson LE, Rojas A, Hao JJ, Kirk WW (2012) Fusarium spp. Causing dry rot of seed potato tubers in Michigan and their sensitivity to fungicides. Plant Dis 96:1767–1774
Gerlach W, Nirenberg H (1982) The Genus Fusarium - a Pictorial Atlas. Mitteilungen aus der Biologischen Bundesanstalt für Land-und Forstwirtschaft (Berlin-Dahlem) 209:1–405
Halstensen AS, Nordby KC, Eduard W, Klemsdal SS (2006) Real-time PCR detection of toxigenic Fusarium in airborne and settled grain dust and associations with trichothecene mycotoxins. J Environ Monit 2006:1235–1241
Heltoft P, Molteberg EL, Nærstad R, Hermansen A (2015) Effect of maturity level and potato cultivar on development of Fusarium dry rot in Norway. Potato Res. doi:10.1007/s11540-015-9300-x
Leach SS, Webb RE (1981) Resistance of selected potato cultivars and clones to Fusarium dry rot. Phytopathology 71:623–629
Lees AK, Cullen DW, Sullivan L, Nicolson MJ (2002) Development of conventional and quantitative real-time PCR assays for the detection and identification of Rhizoctonia solani AG-3 in potato and soil. Plant Pathol 51:293–302
Leslie JF, Summerell BA (2006) The Fusarium laboratory manual. Blackwell Publishing, Oxford
Møllerhagen P (2014) Sorter og sortsprøving i potet 2013. Bioforsk FOKUS 9:292–310
Nicholson P, Simpson DR, Weston G, Rezanoor HN, Lees AK, Parry DW, Joyce D (1998) Detection and quantification of Fusarium culmorum and Fusarium graminearumin cereals using PCR assays. Physiol Mol Plant Pathol 53:17–37
Nicolaisen M, Suproniene S, Nielsen LK, Lazzaro I, Spliid NH, Justesen AF (2009) Real-time PCR for quantification of eleven individual Fusarium species in cereals. J Microbiol Methods 76:234–240
Olofsson J (1976) Vigtiga sjukdomar i potatislager. Vaxtskyddsnotiser 40:40–55
Peters JC, Lees AK, Cullen DW, Sullivan L, Stroud GP, Cunnington AC (2008a) Characterization of Fusarium spp. responsible for causing dry rot of potato in Great Britain. Plant Pathol 57:262–271
Peters R, MacLeod C, Seifert K, Martin R, Hale L, Grau C, MacInnis S (2008b) Pathogenicity to potato tubers of Fusarium spp. Isolated from potato, cereal and forage crops. Am J Potato Res 85:367–374
Ranganna B, Kushalappa AC, Raghavan GSV (1997) Ultraviolet irradiance to control dry rot and soft rot of potato in storage. Can J Plant Pathol 19:30–35
Secor GA, Salas B (2001) Fusarium dry rot and Fusarium wilt. In: Stevenson WR, Loria R, Franc GD, Weingartner DP (eds) Compendium of potato diseases, 2nd edn. APS Press, St. Paul
Seppänen E (1983) Fusariums of the potato in Finland VIII. Occurrence of the pathogens causing potato dry rot and gangrene. Annales Agriculturae Fenniae 22:115–119
Wastie RL, Stewart H, Brown J (1989) Comparative susceptibility of some potato cultivars to dry rot caused by Fusarium sulphureum and F. solani var.coeruleum. Potato Res 32:49–55
Weller SA, Elphinstone JG, Smith NC, Boonham N, Stead DE (2000) Detection of Ralstonia solanacearum strains with a quantitative, multiplex, real-time, Fluorogenic PCR (TaqMan) Assay. Appl Environ Microbiol 66:2853–2858
White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (eds) PCR Protocols: a guide to methods and applications. Academic Press, New York, pp 315–322
Acknowledgments
We would like to thank the agronomists and company representatives who supplied the samples investigated in this survey. We would also like to thank Ragnhild Hustveit, Julien Gomez, and Robert Nybråten for technical assistance. This research was funded by the Foundation for Research Levy on Agricultural Products and the Agricultural Agreement Research Fund, together with Norwegian food industry partners.
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Heltoft, P., Brurberg, M.B., Skogen, M. et al. Fusarium spp. Causing Dry Rot on Potatoes in Norway and Development of a Real-Time PCR Method for Detection of Fusarium coeruleum . Potato Res. 59, 67–80 (2016). https://doi.org/10.1007/s11540-015-9313-5
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DOI: https://doi.org/10.1007/s11540-015-9313-5