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Medical & Biological Engineering & Computing

, Volume 56, Issue 1, pp 85–97 | Cite as

Delivery devices for exposure of biological cells to nanosecond pulsed electric fields

  • Malak Soueid
  • Martinus C. F. Dobbelaar
  • Sabrina Bentouati
  • Sylvia M. Bardet
  • Rodney P. O’Connor
  • Delphine Bessières
  • Jean Paillol
  • Philippe Leveque
  • Delia Arnaud-CormosEmail author
Original Article

Abstract

In this paper, delivery devices for nanosecond pulsed electric field exposure of biological samples in direct contact with electrodes or isolated are presented and characterized. They are based on a modified electroporation cuvette and two transverse electromagnetic cells (TEM cells). The devices were used to apply pulses with high intensity (4.5 kV) and short durations (3 and 13 ns). The delivery devices were electromagnetically characterized in the frequency and time domains. Field intensities of around 5, 0.5, and 12 MV m−1 were obtained by numerical simulations of the biological sample positioned in the three delivery devices. Two delivery systems had a homogenous electric field spatial distribution, and one was adapted to permit a highly localized exposure in the vicinity of a needle. Experimental biological investigations were carried out at different field intensities for five cancer cell lines. The results using flow cytometry showed that cells kept polarized mitochondrial membrane but lost plasma membrane integrity following a dose–response trend after exposure to different electric field intensities. Certain cell types (U87, MCF7) showed higher sensitivities to nsPEFs than other lines tested.

Keywords

Dosimetry In vitro Delivery devices Nanosecond pulsed electric field exposure High voltage 

Notes

Acknowledgements

This research was conducted in the scope of LEA-EBAM, a European Associated Laboratory titled “Pulsed Electric Fields Applications in Biology and Medicine.”

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Copyright information

© International Federation for Medical and Biological Engineering 2017

Authors and Affiliations

  • Malak Soueid
    • 1
  • Martinus C. F. Dobbelaar
    • 1
    • 2
  • Sabrina Bentouati
    • 1
  • Sylvia M. Bardet
    • 1
  • Rodney P. O’Connor
    • 1
  • Delphine Bessières
    • 2
  • Jean Paillol
    • 2
  • Philippe Leveque
    • 1
  • Delia Arnaud-Cormos
    • 1
    Email author
  1. 1.Univ. Limoges, CNRS, XLIMUMR 7252LimogesFrance
  2. 2.SIAME LaboratoryUniversity of PauPauFrance

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