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In vitro evaluation of low-intensity light radiation on murine melanoma (B16F10) cells

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Abstract

Changes in the energy state of biomolecules induced by electromagnetic radiation lead to changes in biological functions of irradiated biomolecules. Using the RRM approach, it was computationally predicted that far-infrared light irradiation in the range of 3500–6000 nm affects biological activity of proto-oncogene proteins. This in vitro study evaluates quantitatively and qualitatively the effects of selected far-infrared exposures in the computationally determined wavelengths on mouse melanoma B16F10 cells and Chinese hamster ovarian (CHO) cells by MTT (thiazolyl blue tetrazolium bromide) cell proliferation assay and confocal laser-scanning microscopy (CLSM). This paper also presents the findings obtained from irradiating B16F10 and CHO cells by the selected wavelengths in visible and near-infrared range. The MTT results show that far-infrared wavelength irradiation induces detrimental effect on cellular viability of B16F10 cells, while that of normal CHO cells is not affected considerably. Moreover, CLSM images demonstrate visible cellular detachment of cancer cells. The observed effects support the hypothesis that far-infrared light irradiation within the computationally determined wavelength range induces biological effect on cancer cells. From irradiation of selected visible and near-infrared wavelengths, no visible changes were detected in cellular viability of either normal or cancer cells.

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Acknowledgments

Design of the exposure device as well as MTT assay has been conducted by P.P. Design of the experiments is done in consultation with E.P, while the experiment setup and all the test preparation are conducted by P.P. The confocal microscopy images are taken by N.M.A. in School of Medical Science at RMIT University. The text of this paper along with Matlab programming and analysis is prepared by P.P, and all other authors read, commented and edited the final manuscript. The project was funded by RMIT School of Electrical and Computer Engineering, and the first author was funded by RMIT PhD scholarship.

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Authors and Affiliations

  1. School of Electrical and Computer Engineering, RMIT University, Melbourne, VIC, Australia

    P. Peidaee & E. Pirogova

  2. Department of Biotechnology and Environmental Biology, School of Applied Sciences, RMIT University, Melbourne, VIC, Australia

    N. M. Almansour

  3. Health Innovation Research Institute, RMIT University, Melbourne, VIC, Australia

    E. Pirogova

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  1. P. Peidaee
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  2. N. M. Almansour
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  3. E. Pirogova
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Correspondence to P. Peidaee.

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Peidaee, P., Almansour, N.M. & Pirogova, E. In vitro evaluation of low-intensity light radiation on murine melanoma (B16F10) cells. Med Biol Eng Comput 54, 325–332 (2016). https://doi.org/10.1007/s11517-015-1313-8

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  • DOI: https://doi.org/10.1007/s11517-015-1313-8

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