Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions
- 10 Downloads
The possibility of dietary ginger to enhance oxidative stress resistance and to extend life span was studied on Drosophila melanogaster.
Oxidative stress was induced by a reducing agent dithiothreitol. Experimental groups of male D. melanogaster were cultured on media containing: 1) no additive; 2) dithiothreitol, added into the nutritional mixture to the final concentration of 10 mM; 3) 25 mg of ginger powder g–1 of the nutritional mixture; and 4) 10 mM of dithiothreitol and 25 mg of ginger powder g–1 of the nutritional mixture. The number of alive fruit flies was inspected daily, and mean life span was determined for each experimental group.
The addition of dithiothreitol to D. melanogaster nutritional mixture was established to result in an increase in concentration of two markers of oxidative stress conditions (thiobarbituric acid reactive substances as products of lipid peroxidation and carbonylated proteins as products of protein oxidation) in fly tissues. It was followed by significant reduction of mean life span and maximum life span of the last 10% of flies. Plant preparation, being added simultaneously with dithiothreitol, significantly diminished the negative effects of this xenobiotic. In conditions of additional stress load induced by hydrogen peroxide or high temperature, survival of insects treated with dithiothreitol on the background of ginger powder was the highest.
Thus, the presented data give the evidence that ginger preparations can reduce oxidative stress outcomes and significantly increase the life expectancy of fruit flies in stress conditions.
Keywordsginger oxidative stress Drosophila melanogaster
Unable to display preview. Download preview PDF.
- Broughton S J, Piper M D, Ikeya T, Bass T M, Jacobson J, Driege Y, Martinez P, Hafen E, Withers D J, Leevers S J, Partridge L (2005). Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands. Proc Natl Acad Sci USA, 102(8): 3105–3110CrossRefPubMedPubMedCentralGoogle Scholar
- Ezeonu C S, Egbuna P A C, Ezeanyika L U S, Nkwonta C G, Idoko N D (2011). Antihepatotoxicity studies of crude extract of Zingiber offinale on CCl4 induced toxicity and comparison of the extract’s fraction D hepatoprotective capacity. Res J Med Sci, 5(2): 102–107Google Scholar
- Haub C (2011). World Population Aging: Clocks Illustrate Growth in Population Under Age 5 and Over Age 65. http://www.prb.org/Publications/Articles/2011/agingpopulationclocks.aspxGoogle Scholar
- Oboh G, Akinyemi A J, Ademiluyi A O (2012). Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol, 64(1-2): 31–36Google Scholar