Frontiers in Biology

, Volume 13, Issue 2, pp 130–136 | Cite as

Zingiber officinale extends Drosophila melanogaster life span in xenobiotic-induced oxidative stress conditions

  • Volodymyr Padalko
  • Viktoriya Dzyuba
  • Olena Kozlova
  • Hanna Sheremet
  • Olena Protsenko
Research Article



The possibility of dietary ginger to enhance oxidative stress resistance and to extend life span was studied on Drosophila melanogaster.


Oxidative stress was induced by a reducing agent dithiothreitol. Experimental groups of male D. melanogaster were cultured on media containing: 1) no additive; 2) dithiothreitol, added into the nutritional mixture to the final concentration of 10 mM; 3) 25 mg of ginger powder g–1 of the nutritional mixture; and 4) 10 mM of dithiothreitol and 25 mg of ginger powder g–1 of the nutritional mixture. The number of alive fruit flies was inspected daily, and mean life span was determined for each experimental group.


The addition of dithiothreitol to D. melanogaster nutritional mixture was established to result in an increase in concentration of two markers of oxidative stress conditions (thiobarbituric acid reactive substances as products of lipid peroxidation and carbonylated proteins as products of protein oxidation) in fly tissues. It was followed by significant reduction of mean life span and maximum life span of the last 10% of flies. Plant preparation, being added simultaneously with dithiothreitol, significantly diminished the negative effects of this xenobiotic. In conditions of additional stress load induced by hydrogen peroxide or high temperature, survival of insects treated with dithiothreitol on the background of ginger powder was the highest.


Thus, the presented data give the evidence that ginger preparations can reduce oxidative stress outcomes and significantly increase the life expectancy of fruit flies in stress conditions.


ginger oxidative stress Drosophila melanogaster 


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  1. Ahmed R S, Suke S G, Seth V, Chakraborti A, Tripathi A K, Banerjee B D (2008). Protective effects of dietary ginger (Zingiber officinales Rosc.) on lindane-induced oxidative stress in rats. Phytother Res, 22 (7): 902–906CrossRefPubMedGoogle Scholar
  2. Ajith T A (2010). Ameliorating reactive oxygen species-induced in vitro lipid peroxidation in brain, liver, mitochondria and DNA damage by Zingiber officinale Roscoe. Indian J Clin Biochem, 25(1): 67–73CrossRefPubMedPubMedCentralGoogle Scholar
  3. Al-Amin Z M, Thomson M, Al-Qattan K K, Peltonen-Shalaby R, Ali M (2006). Anti-diabetic and hypolipidaemic properties of ginger (Zingiber officinale) in streptozotocin-induced diabetic rats. Br J Nutr, 96(4): 660–666CrossRefPubMedGoogle Scholar
  4. Baliga MS, Haniadka R, Pereira MM, Thilakchand K R, Rao S, Arora R (2012). Radioprotective effects of Zingiber officinale Roscoe (ginger): past, present and future. Food Funct, 3(7): 714–723CrossRefPubMedGoogle Scholar
  5. Broughton S J, Piper M D, Ikeya T, Bass T M, Jacobson J, Driege Y, Martinez P, Hafen E, Withers D J, Leevers S J, Partridge L (2005). Longer lifespan, altered metabolism, and stress resistance in Drosophila from ablation of cells making insulin-like ligands. Proc Natl Acad Sci USA, 102(8): 3105–3110CrossRefPubMedPubMedCentralGoogle Scholar
  6. Ezeonu C S, Egbuna P A C, Ezeanyika L U S, Nkwonta C G, Idoko N D (2011). Antihepatotoxicity studies of crude extract of Zingiber offinale on CCl4 induced toxicity and comparison of the extract’s fraction D hepatoprotective capacity. Res J Med Sci, 5(2): 102–107Google Scholar
  7. Gaddam D, Stevens N, Hollien J (2013). Comparison of mRNA localization and regulation during endoplasmic reticulum stress in Drosophila cells. Mol Biol Cell, 24(1): 14–20CrossRefPubMedPubMedCentralGoogle Scholar
  8. Grotewiel M S, Martin I, Bhandari P, Cook-Wiens E (2005). Functional senescence in Drosophila melanogaster. Ageing Res Rev, 4(3): 372–397CrossRefPubMedGoogle Scholar
  9. Haub C (2011). World Population Aging: Clocks Illustrate Growth in Population Under Age 5 and Over Age 65. Scholar
  10. Higa A, Chevet E (2012). Redox signaling loops in the unfolded protein response. Cell Signal, 24(8): 1548–1555CrossRefPubMedGoogle Scholar
  11. Jin K (2010). Modern biological theories of aging. Aging Dis, 1(2): 72–74PubMedPubMedCentralGoogle Scholar
  12. Kregel K C, Zhang H J (2007). An integrated view of oxidative stress in aging: basic mechanisms, functional effects, and pathological considerations. Am J Physiol Regul Integr Comp Physiol, 292(1): R18–R36CrossRefPubMedGoogle Scholar
  13. Le Bourg E (1987). The rate of living theory. Spontaneous locomotor activity, aging and longevity in Drosophila melanogaster. Exp Gerontol, 22(5): 359–369PubMedGoogle Scholar
  14. Levine R L, Garland D, Oliver C N, Amici A, Climent I, Lenz A G, Ahn B W, Shaltiel S, Stadtman E R (1990). Determination of carbonyl content in oxidatively modified proteins. Methods Enzymol, 186: 464–478CrossRefPubMedGoogle Scholar
  15. Lithgow G J, White T M, Melov S, Johnson T E (1995). Thermotolerance and extended life-span conferred by single-gene mutations and induced by thermal stress. Proc Natl Acad Sci USA, 92 (16): 7540–7544CrossRefPubMedPubMedCentralGoogle Scholar
  16. Long J, Gao H, Sun L, Liu J, Zhao-Wilson X (2009). Grape extract protects mitochondria from oxidative damage and improves locomotor dysfunction and extends lifespan in a Drosophila Parkinson’s disease model. Rejuvenation Res, 12(5): 321–331CrossRefPubMedGoogle Scholar
  17. Lowry O H, Rosebrough N J, Farr A L, Randall R J (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193(1): 265–275PubMedPubMedCentralGoogle Scholar
  18. Miller G L (1959). Protein determination for large numbers of samples. Anal Chem, 31(5): 964CrossRefGoogle Scholar
  19. Oboh G, Akinyemi A J, Ademiluyi A O (2012). Antioxidant and inhibitory effect of red ginger (Zingiber officinale var. Rubra) and white ginger (Zingiber officinale Roscoe) on Fe(2+) induced lipid peroxidation in rat brain in vitro. Exp Toxicol Pathol, 64(1-2): 31–36Google Scholar
  20. Ohkawa H, Ohishi N, Yagi K (1979). Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem, 95(2): 351–358CrossRefPubMedGoogle Scholar
  21. Peng C, Chan H Y, Huang Y, Yu H, Chen Z Y (2011). Apple polyphenols extend the mean lifespan of Drosophila melanogaster. J Agric Food Chem, 59(5): 2097–2106CrossRefPubMedGoogle Scholar
  22. Rawal S, Singh P, Gupta A, Mohanty S (2014). Dietary intake of Curcuma longa and Emblica officinalis increases life span in Drosophila melanogaster. BioMed Res Int, 2014: 910290CrossRefPubMedPubMedCentralGoogle Scholar
  23. Seugé J, Laugé G, Ferradini C, Deysine A (1985). Accelerated aging of the insect Drosophila melanogaster by g irradiations of pupae. Exp Gerontol, 20(2): 131–139CrossRefPubMedGoogle Scholar
  24. Shirpoor A, Rezaei F, Fard A A, Afshari A T, Gharalari F H, Rasmi Y (2016). Ginger extract protects rat’s kidneys against oxidative damage after chronic ethanol administration. Biomed Pharmacother, 84: 698–704CrossRefPubMedGoogle Scholar
  25. Speciale A, Chirafisi J, Saija A, Cimino F (2011). Nutritional antioxidants and adaptive cell responses: an update. Curr Mol Med, 11(9): 770–789CrossRefPubMedGoogle Scholar

Copyright information

© Higher Education Press and Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Volodymyr Padalko
    • 1
  • Viktoriya Dzyuba
    • 2
  • Olena Kozlova
    • 2
  • Hanna Sheremet
    • 2
  • Olena Protsenko
    • 1
  1. 1.School of MedicineV.N. Karazin Kharkiv National UniversityKharkivUkraine
  2. 2.Department of Membrane Biophysics, Research Institute of BiologyV.N. Karazin Kharkiv National UniversityKharkivUkraine

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