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Analysis of p53 expression in partial hydatidiform mole and hydropic abortion

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Frontiers in Biology



Gestational trophoblastic disease (GTD) is a heterogeneous group of disorders characterized by abnormal trophoblast tissue. Molar and non-molar hydropic placental changes are the most common forms of GTD. Differential diagnosis of GTD is sometimes problematic. Recently, p53 expression was identified as a good marker for distinguishing GTD types.


Comparison of p53 expression in partial hydatidiform mole (PHM) and hydropic abortion.


In this prospective cross-sectional study, molar and non-molar hydropic pregnancy specimens were collected. Immunohistochemical staining, based on the Labeled Streptavidin Biotin (LSAB) technique, was carried out on multiple 4 mm paraffin block sections prepared from formalin-fixed trophoblastic tissues. Polymer-based Envision was used to assess p53 tumor suppressor protein immunoreactivity. p53 expression was then compared between both groups.


In the study, 40 patients were included: 20 with confirmed PHM and 20 with hydropic pregnancy. p53 protein was positive in 60% of patients with PHM and 25% of patients with hydropic pregnancy. The p53 positive rate was significantly higher in patients with PHM (p = 0.027). Moreover, patients with PHM had a significantly high grade of staining (p<0.001).


Our findings indicate that immunohistochemical analysis of p53 protein can be used to distinguish PHM and hydropic pregnancy.

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We wish to thank all our colleagues at Imam Khomeini and Golestan Hospitals, Ahvaz, Iran.

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Correspondence to Parvin Kheradmand.

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Kheradmand, P., Goudarzi, M. & Tavakoli, M. Analysis of p53 expression in partial hydatidiform mole and hydropic abortion. Front. Biol. 12, 357–360 (2017).

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