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Functional protein microarray: an ideal platform for investigating protein binding property

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Frontiers in Biology

Abstract

Functional protein microarray is an important tool for high-throughput and large-scale systems biology studies. Besides the progresses that have been made for protein microarray fabrication, significant advancements have also been achieved for applying protein microarrays on determining a variety of protein biochemical activities. Among these applications, detection of protein binding properties, such as protein-protein interactions (PPIs), protein-DNA interactions (PDIs), protein-RNA interactions, and antigen-antibody interactions, are straightforward and have substantial impacts on many research fields. In this review, we will focus on the recent progresses in protein-protein, protein-DNA, protein-RNA, protein-small molecule, protein-lipid, protein-glycan, and antigen-antibody interactions. We will also discuss the challenges and future directions of protein microarray technologies. We strongly believe that protein microarrays will soon become an indispensible tool for both basic research and clinical applications.

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Authors and Affiliations

  1. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai, 200240, China

    Shu-Min Zhou, Li Cheng, Shu-Juan Guo & Sheng-Ce Tao

  2. State Key Laboratory of Oncogenes and Related Genes, Shanghai, 200240, China

    Shu-Min Zhou, Li Cheng, Shu-Juan Guo & Sheng-Ce Tao

  3. Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Heng Zhu

  4. The High-Throughput Biology Center, Johns Hopkins University School of Medicine, Baltimore, MD, 21205, USA

    Heng Zhu

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  1. Shu-Min Zhou
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  2. Li Cheng
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  4. Heng Zhu
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Correspondence to Heng Zhu or Sheng-Ce Tao.

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Zhou, SM., Cheng, L., Guo, SJ. et al. Functional protein microarray: an ideal platform for investigating protein binding property. Front. Biol. 7, 336–349 (2012). https://doi.org/10.1007/s11515-012-1236-9

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