Frontiers of Biology in China

, Volume 1, Issue 3, pp 290–294 | Cite as

Karyotype analysis and physical mapping of 45S rDNA in eight species of Sophora, Robinia, and Amorpha

  • Liu Bo 
  • Chen Chengbin 
  • Li Xiulan 
  • Qi Liwang 
  • Han Suying 
Research Article


The karyotype analysis and physical locations of 45S rDNA were carried out by means of fluorescence in situ hybridization in three species, and two forms of Sophora, two species of Robina, and one species of Amorpha. S. japonica L., S. japonica L. f. oligophylla Franch., S. japonica L. f. pendula Loud., and S. xanthantha C. Y. Ma. are all tetraploids with 2n = 28. There were four 45S rDNA sites in pericentromeric regions of two pairs of chromosomes in each of them. S. rubriflora Tsoong. is a triploid with 2n = 21, and three sites were located in each satellite of group 5 chromosomes. In R. pseudoacacia L. (2n = 2x = 22), we examined four intensive signals in telomeric regions of two pairs of satellite chromosomes. In R. hispida L. (2n = 2x = 30), there were four other signals in centromeric regions besides those like in R. pseudoacacia. Amorpha fruticosa L. has most chromosomes (2n = 40) among the eight materials, however, there were only six 45S rDNA loci and they laid in centromeric regions, and satellites of three pairs of chromosomes. 45S rDNA is a valuable chromosomal landmark in karyotype analysis. The distribution and genomic organization of rDNA in the three genera were also discussed.


Sophora Robinia Amorpha karyotype analysis 45S rDNA fluorescence in situ hybridization (FISH) 


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Copyright information

© Higher Education Press and Springer-Verlag 2006

Authors and Affiliations

  • Liu Bo 
    • 1
  • Chen Chengbin 
    • 1
  • Li Xiulan 
    • 1
  • Qi Liwang 
    • 2
  • Han Suying 
    • 2
  1. 1.Department of Biology, College of Life SciencesNankai UniversityTianjinChina
  2. 2.Laboratory of Cell Biology, Research Institute of ForestryChinese Academy of ForestryBeijingChina

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