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Effect of a polyphenol molecular size on the gluten proteins – polyphenols interactions studied with FT-Raman spectroscopy

Food Biophysics volume 17, pages 535–544 (2022)Cite this article

Abstract

Changes observed in the structure of gluten network as a results of dough supplementation with polyphenols may depend on the number and type of functional groups at the aromatic ring, antioxidant activity and size of the polyphenol molecule. The present studies show effect of polyphenols differing in the molecular size (gallic, ellagic and tannic acids) on the structure of gluten network studied with FT- Raman spectroscopy. The model dough was supplemented with the polyphenols in the amount of 0.05%, 0.1% and 0.2%. Farinographic studies showed that only gallic and ellagic acids led to dough breakdown, whereas tannic acid (the biggest molecule) stabilized the gluten network. Spectroscopic results indicate formation of covalent or hydrogen bonds between protein SH groups and polyphenol OH groups. Moreover, it was observed a strong negative band in all spectra that can be assigned to α-helices. It suggests that structural changes can concern mainly gliadins. When it comes to aromatic amino acids, the pattern of tyrosine hydrogen bonding was not affected by the polyphenols, whereas microenvironment of tryptophan changed considerably to more hydrophobic. It may indicate folding of polypeptide chains and development of more compact and ordered gluten network. The results indicated that disulphide bridges can be broken during the dough mixing and phenolic molecules formed bonds with SH groups of cysteine. This confirms inclusion of the polyphenols into the gluten network.

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This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.

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Authors and Affiliations

  1. Institute of Agrophysics, Polish Academy of Sciences, Doświadczalna 4, 20-290, Lublin, Poland

    Magdalena Krekora & Agnieszka Nawrocka

Authors
  1. Magdalena Krekora
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  2. Agnieszka Nawrocka
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Contributions

Magdalena Krekora: Conceptualization, Methodology, Investigation, Formal analysis, Visualization, Writing - original draft, Writing - review & editing. Agnieszka Nawrocka: Conceptualization, Supervision, Methodology, Writing - original draft, Writing - review & editing.

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Correspondence to Agnieszka Nawrocka.

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Krekora, M., Nawrocka, A. Effect of a polyphenol molecular size on the gluten proteins – polyphenols interactions studied with FT-Raman spectroscopy. Food Biophysics 17, 535–544 (2022). https://doi.org/10.1007/s11483-022-09740-z

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  • DOI: https://doi.org/10.1007/s11483-022-09740-z

Keywords

  • Gluten structure
  • Phenolic acid
  • Tannins
  • FT-Raman