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The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study

Food Biophysics volume 5, pages 17–23 (2010)Cite this article

Abstract

The scientific evaluation of the antidiabetic efficacy of aqueous extract of Trichosanthes dioica fruits on streptozotocin-induced diabetic rats is being presented. The graded doses of the extract, viz., 500, 750, 1,000, and 1,250 mg/kg body weight (bw), were administered orally, and it was observed that the blood glucose concentration decreased in a dose-dependent manner. The dose of 1,000 mg/kg bw showed the maximum fall of 23.8% and 19.1% in blood glucose level (BGL) during fasting BGL and glucose tolerance test (GTT) studies, respectively, of nondiabetic rats. Whereas in the case of subdiabetic and mild diabetic models, the same dose showed reduction in BGL of 22.0% and 31.4% during GTT. The study also involves the first use of laser-induced breakdown spectroscopy as a sensitive analytical tool to detect the elemental profile responsible for the antidiabetic activity of aqueous extract of T. dioica fruits that exhibits the antidiabetic activity. High intensities of Ca, Mg, and Fe indicate large concentrations of these elements in the extract, since according to Boltzmann’s distribution law, intensities are directly proportional to concentrations. The higher concentrations of these glycemic elements, viz. Ca, Mg, and Fe, are responsible for the antidiabetic potential of T. dioica as well as other plant already reported by our research group.

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Acknowledgements

The authors are grateful to DRDO and NMPB, New Delhi, India, for providing the financial assistance. The first author PKR is thankful to ICMR for the award of senior research fellowship.

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Authors and Affiliations

  1. Alternative Therapeutics Unit, Drug Discovery and Development Division, Medicinal Research Lab, Department of Chemistry, University of Allahabad, Allahabad, 211002, India

    Prashant Kumar Rai, Sanjukta Chatterji & Geeta Watal

  2. Laser Spectroscopy Research Laboratory, Department of Physics, University of Allahabad, Allahabad, 211002, India

    Nilesh K. Rai & Awadhesh K. Rai

  3. Laboratory of Biophysics, Department of Agrotechnology and Food Sciences, Wageningen University, Dreijenlaan 3-Transitorium, 6703 HA, Wageningen, The Netherlands

    Dane Bicanic

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  1. Prashant Kumar Rai
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  2. Sanjukta Chatterji
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  3. Nilesh K. Rai
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  4. Awadhesh K. Rai
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  5. Dane Bicanic
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  6. Geeta Watal
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Correspondence to Geeta Watal.

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Rai, P.K., Chatterji, S., Rai, N.K. et al. The Glycemic Elemental Profile of Trichosanthes dioica: A LIBS-Based Study. Food Biophysics 5, 17–23 (2010). https://doi.org/10.1007/s11483-009-9139-2

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  • DOI: https://doi.org/10.1007/s11483-009-9139-2

Keywords

  • Diabetes mellitus
  • Glycemic elements
  • LIBS
  • Streptozotocin
  • Albino Wistar rats
  • Trichosanthes dioica