Immunoproteasome Inhibitor ONX-0914 Affects Long-Term Potentiation in Murine Hippocampus

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The study was supported by the Russian Science Foundation grant 18–74-10095, electrophysiological experiments were supported by Russian Ministry of Science and Education (agreement #075–15–2020-801). The immunofluorescence was performed using equipment of the Core Centrum of Institute of Developmental Biology RAS. The work of AT, IV and LYu was supported by Government program of basic research in Koltzov Institute of Developmental Biology, Russian Academy of Sciences in 2020, No. 0108–2019–0002.

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All authors contributed to the study conception and design. The study was designed by Morozov Alexey, Bal Natalia and Karpov Vadim. Material preparation, data collection and analysis were performed by Maltsev Alexander, Funikov Sergei, Burov Alexander, Spasskaya Daria, Ignatyuk Vasilina, Astakhova Tatjana, Lyupina Yu., Tutyaeva Vera, Deikin Alexey, Bal Natalia, Karpov Vadim and Morozov Alexey. The first draft of the manuscript was written by Morozov Alexey and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Alexey Morozov.

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Authors declare no conflicts of interests.

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All experiments were performed in accordance with the European Convention for the Protection of Vertebrate Animals Used for Experimental and Other Scientific Research Purposes 1986 86/609 / EEC and the institutional requirements for the care and use of laboratory animals (Institute of Higher Nervous Activity and Neurophysiology, RAS, Russia).

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Suppl. Figure. Analysis of the anti-β5i antibodies. a. The antibodies were obtained after immunization of rabbits with the C-terminal murine β5i peptide (in red) conjugated with ovalbumin. After the third immunization animal sera were analyzed by immunoblotting with the 20S immunoproteasome (Enzo, USA) as the antigen (b). All animal sera were collected, and specific antibodies were obtained by affinity chromatography and tested as above (c). Immunoblotting of the purified recombinant β5i-subunit with the obtained antibodies (d). (PPTX 3593 kb)

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Maltsev, A., Funikov, S., Burov, A. et al. Immunoproteasome Inhibitor ONX-0914 Affects Long-Term Potentiation in Murine Hippocampus. J Neuroimmune Pharmacol (2021).

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  • Proteasome
  • Immunoproteasome
  • Proteasome inhibitor
  • ONX-0914
  • Synaptic plasticity