Abstract
We developed a nanotechnology based-cell mediated drug delivery system by loading myelin antigen-specific T cells with nanoparticles bound to anti-CD20 monoclonal antibody. Anti-CD20 antibody is a current treatment (ocrelizumab) for multiple sclerosis (MS), a chronic, inflammatory and autoimmune disease of the central nervous system (CNS). CD20-depletion has been associated with efficacy in active relapsing and progressive MS, but may not efficiently target inflammatory cells compartmentalized in the CNS. In our work, the intravenous transfer of T cells containing nanoparticle-anti-CD20 complex in mice causes B cell depletion in the spleen and in the brain, whereas the injection of anti-CD20 alone depletes B cells only in the spleen. Testing this system in Experimental Autoimmune Encephalomyelitis (EAE), animal model of MS, we found that spinal cord B cell depletion ameliorates the disease course and pathology.
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Acknowledgements
We are particularly grateful to Laura Ballerini for the critical revision.
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This work was in part supported by Regione Toscana, project INSIDE, FESR 2014–2020.
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Cericol Research Center Colorobbia (Italy) provided nanoparticles, named NBR, used in this work.
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Carnasciali, A., Amoriello, R., Bonechi, E. et al. T Cell Delivery of Nanoparticles-Bound Anti-CD20 Monoclonal Antibody: Successful B Cell Depletion in the Spinal Cord during Experimental Autoimmune Encephalomyelitis. J Neuroimmune Pharmacol 16, 376–389 (2021). https://doi.org/10.1007/s11481-020-09931-w
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DOI: https://doi.org/10.1007/s11481-020-09931-w