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Analysis of Biomolecular Interaction Process Based on SPR Imaging Method in Microfluidic Chips

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Abstract

Molecular dynamics characteristics have important significance in studying the interaction between biomolecules, such as drug screening, environmental monitoring and evaluation of virus infectivity. In this work, a simulation method based on step function is proposed to study the molecular binding and dissociation processes, which is further coupled with surface plasmon wave evanescent field. A complete simulation model is established from biomolecular reaction to surface plasmon resonance (SPR) optical detection, which is consistent with the experimental process. Furthermore, experimental verification studies were carried out on human serum albumin (HSA), a marker of the urinary protein disease. The effects of microchannel size, liquid flow rate, molecular concentration, complex samples and other conditions on the molecular dynamics binding characteristics were studied under the conditions of antibody fixation and antigen–antibody binding reaction. Optimized parameters are obtained such as microchannel size and liquid flow rate, which can save reaction reagents reduce the experimental process and improve the experimental detection accuracy. Therefore, the established simulation and experimental methods include a complete process from biological information to optical information, which is expected to be a powerful tool for molecular interaction analysis in the future.

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Availability of Data and Material

The data and material that support the findings of this study are available from the corresponding author upon reasonable request.

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Funding

The authors greatly acknowledge the financial support from the Natural Science Foundation of Beijing Municipality (4202053), Joint Project of BRC-BC (Biomedical Translational Engineering Research Center of BUCT-CJFH) (XK2020-15) and the National Natural Science Foundation of China (61971026, 81871505). This work was also funded by Fundamental Research Funds for the Central Universities (BUCTRC201905).

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Authors and Affiliations

  1. College of Information Science and Technology, Beijing University of Chemical Technology, Beijing, 100029, China

    Honggang Wang, Huixiang Wang, Hao Zhang, Yafeng Huang, Xianbo Qiu, Duli Yu & Lulu Zhang

  2. The Key Laboratory of Optoelectronics Information and Technology, Tianjin University, Tianjin, 300072, China

    Zhen Tian

Authors
  1. Honggang Wang
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  2. Huixiang Wang
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  3. Zhen Tian
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  4. Hao Zhang
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  6. Xianbo Qiu
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  7. Duli Yu
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  8. Lulu Zhang
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Contributions

H.G.W. and H.X.W. were responsible for the simulation, experiment and original draft. Zhen Tian: Resources, simulation, supervision and reviewing. H.Z. prepared the sample and processed experiment data. Y.H. was responsible for experimental software design. X.Q. and D.Y. provided the funding. L.Z. provided the funding and reviewed and revised the manuscript.

Corresponding author

Correspondence to Lulu Zhang.

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Wang, H., Wang, H., Tian, Z. et al. Analysis of Biomolecular Interaction Process Based on SPR Imaging Method in Microfluidic Chips. Plasmonics 17, 621–631 (2022). https://doi.org/10.1007/s11468-021-01548-w

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  • DOI: https://doi.org/10.1007/s11468-021-01548-w

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