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Transferrin-Mediated Glioblastoma Cell Targeting of Hexagonal Boron Nitrides

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Abstract

Hexagonal boron nitrides (hBNs) are promising nanomaterials with their high boron content, non-toxic nature in inactive form, high chemical stability, and mechanical strength. However, their hydrophobic nature limits their use in biomedical applications. Therefore, the hBNs have been functionalized with DSPE-PEG-NH2 to increase their colloidal stability and circulation time in bloodstream as well as to provide active sites on their surface for further functionalization with tumor-targeting agents. Then, further functionalization of the DSPE-PEG-hBNs with transferrin (TfR) was applied for selective targeting of transferrin receptors overexpressed by brain tumor cells. After that, the cellular interaction and biocompatibility of the structure was investigated on glioblastoma multiforme (U87MG) cancer cells. The cellular investigations showed that transferrin functionalization of the DSPE-PEG-hBNs increased their uptake by glioblastoma cancer cells and decreased cell viability due to the enhanced cellular internalization. Based on the data, the TfR-DSPE-PEG-hBNs are promising agents to evaluate them in drug carrying and targeting applications.

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Funding

This study received financial support from Yeditepe University.

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Author notes

  1. Melis Emanet and Özlem Şen contributed equally to this work.

Authors and Affiliations

  1. Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Ataşehir, 34755, Istanbul, Turkey

    Melis Emanet, Özlem Şen & Mustafa Çulha

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  1. Melis Emanet
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  2. Özlem Şen
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  3. Mustafa Çulha
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Correspondence to Mustafa Çulha.

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Emanet, M., Şen, Ö. & Çulha, M. Transferrin-Mediated Glioblastoma Cell Targeting of Hexagonal Boron Nitrides. Plasmonics 15, 1543–1549 (2020). https://doi.org/10.1007/s11468-020-01206-7

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