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Plasmonics

pp 1–8 | Cite as

Development of Metal-Enhanced Fluorescence-Based Aptasensor for Thrombin Detection Using Silver Dendritic Nanostructures

  • Arezoo Lotfi
  • Maryam Nikkhah
  • Ahmad Moshaii
Article
  • 45 Downloads

Abstract

Metal-enhanced fluorescence (MEF) phenomenon has shown a promising potential in the field of fluorescence-based biological sensing. In this study, we optimized the electroless metal deposition method to fabricate silver dendritic nanostructures as effective MEF active substrates. Then, an aptasensor was developed for thrombin detection using the established surfaces. For this purpose, thiolated 29-mer thrombin-binding aptamers (TBA29 (12T) SH) as capturing aptamer were immobilized on the surface of silver dendritic nanostructures, then thrombin was sandwiched between the capturing aptamer and Cy5-labeled 15-mer thrombin aptamer (TBA15-Cy5). Quantitative analysis was performed through fluorescence signal measurement. The established aptasensor presented satisfactory sensitivity and selectivity and exhibited a limit of detection (LOD) as low as 32 pM. This aptasensor was also able to detect thrombin in the human serum at picomolar levels. Furthermore, the ease and relatively low-cost of fabrication of this platform introduce it as a tool with great potential for the clinical diagnosis of diseases and also for improving sensitivity of a variety of technologies which exploit fluorescent dyes for analyte detection, at ultra-trace levels, in complex matrices.

Keywords

Silver dendritic nanostructure Metal-enhanced fluorescence (MEF) Thrombin Aptamer 

Notes

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Ethics Approval

The experimental protocol was approved by the research ethics committee of Tarbiat Modares University. The participant provided written informed consent.

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Nanobiotechnology, Faculty of Biological SciencesTarbiat Modares UniversityTehranIran
  2. 2.Department of Physics, Faculty of Basic SciencesTarbiat Modares UniversityTehranIran

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