A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single-nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Price excludes VAT (USA)
Tax calculation will be finalised during checkout.
Shipway AN, Katz E, Willner I (2000) Nanoparticle arrays on surfaces for electronic, optical, and sensor applications. Chem Phys Chem 1(1):18–52
Niemeyer CM (2001) Nanoparticles, proteins, and nucleic acids: biotechnology meets materials science. Angew Chem Int Ed 40(22):4128–4158
Nam J-M, Thaxton CS, Mirkin CA (2003) Nanoparticle-based Bio-Bar codes for the ultrasensitive detection of proteins. Science 301(5641):1884–1886
Agasti SS et al (2010) Nanoparticles for detection and diagnosis. Adv Drug Deliv Rev 62(3):316–328
Hutter E, Fendler JH (2004) Exploitation of localized surface plasmon resonance. Adv Mater 16(19):1685–1706
Daniel MC, Astruc D (2004) Gold nanoparticles: assembly, supramolecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev 104(1):293–346
Wilson R (2008) The use of gold nanoparticles in diagnostics and detection. Chem Soc Rev 37(9):2028–2045
Sperling RA et al (2008) Biological applications of gold nanoparticles. Chem Soc Rev 37(9):1896–1908
Zhao W, Brook MA, Li YF (2008) Design of gold nanoparticle-based colorimetric biosensing assays. Chembiochem 9(15):2363–2371
Giljohann DA et al (2010) Gold nanoparticles for biology and medicine. Angew Chem Int Ed 49(19):3280–3294
Hubble LJ et al (2012) Gold nanoparticle chemiresistors operating in biological fluids. Lab Chip 12(17):3040–3048
Ozhikandathil J, Badilescu S, Packirisamy M (2012) Gold nanoisland structures integrated in a lab-on-a-chip for plasmonic detection of bovine growth hormone. J Biomed Opt 17(7):077011–0770019
Zhang Y et al (2012) Towards a high-throughput label-free detection system combining localized-surface plasmon resonance and microfluidics. Lab Chip 12(17):3012–3015
Hiep HM et al (2007) A localized surface plasmon resonance based immunosensor for the detection of casein in milk. Sci Technol Adv Mater 8(4):331–338
Huang CJ et al (2009) Localized surface plasmon resonance biosensor integrated with microfluidic chip. Biomed Microdevices 11(4):893–901
Guo LH, Kim DH (2012) LSPR biomolecular assay with high sensitivity induced by aptamer-antigen-antibody sandwich complex. Biosens Bioelectron 31(1):567–570
Guo L (2013) Distance-mediated plasmonic dimers for reusable colorimetric switches: a measurable peak shift of over 60 nm. Small 9(2):234–240
Guo L, Kim D-H (2011) Reusable plasmonic aptasensors: using a single nanoparticle to establish a calibration curve and to detect analytes. Chem Commun 47(25):7125–7127
Nusz GJ et al (2008) Label-free plasmonic detection of biomolecular binding by a single gold nanorod. Anal Chem 80(4):984–989
Nusz GJ et al (2009) Rational selection of gold nanorod geometry for label-free plasmonic biosensors. Acs Nano 3(4):795–806
Truong PL et al (2011) A new method for non-labeling attomolar detection of diseases based on an individual gold nanorod immunosensor. Lab Chip 11(15):2591–2597
Guo LH et al (2011) In situ assembly, regeneration and plasmonic immunosensing of a Au nanorod monolayer in a closed-surface flow channel. Lab Chip 11(19):3299–3304
Hiep HM et al (2008) A microfluidic chip based on localized surface plasmon resonance for real-time monitoring of antigen-antibody reactions. Jpn J Appl Phys 47(2):1337–1341
Geng ZX et al Theoretical analysis and fabrication of PDMS-based surface plasmon resonance sensor chips. 2009 4th Ieee International Conference on Nano/Micro Engineered and Molecular Systems, Vols 1 and 2. 2009, New York: Ieee. 51–54
Luo Y et al (2011) Superlocalization of single molecules and nanoparticles in high-fidelity optical imaging microfluidic devices. Anal Chem 83(13):5073–5077
Unger MA et al (2000) Monolithic microfabricated valves and pumps by multilayer soft lithography. Science 288(5463):113–116
Thorsen T, Maerkl SJ, Quake SR (2002) Microfluidic large-scale integration. Science 298(5593):580–584
Jana NR, Gearheart L, Murphy CJ (2001) Seed-mediated growth approach for shape-controlled synthesis of spheroidal and rod-like gold nanoparticles using a surfactant template. Adv Mater 13(18):1389–1393
Nikoobakht B, El-Sayed MA (2003) Preparation and growth mechanism of gold nanorods (NRs) using seed-mediated growth method. Chem Mater 15(10):1957–1962
Guo LH, Zhou XD, Kim DH (2011) Facile fabrication of distance-tunable Au-nanorod chips for single-nanoparticle plasmonic biosensors. Biosens Bioelectron 26(5):2246–2251
Tombelli S, Minunni A, Mascini A (2005) Analytical applications of aptamers. Biosens Bioelectron 20(12):2424–2434
Bunka DHJ, Stockley PG (2006) Aptamers come of age—at last. Nat Rev Microbiol 4(8):588–596
Willner I, Zayats M (2007) Electronic aptamer-based sensors. Angew Chem Int Ed 46(34):6408–6418
Liu JW, Cao ZH, Lu Y (2009) Functional nucleic acid sensors. Chem Rev 109(5):1948–1998
Guo LH et al (2011) Nanoarray-based biomolecular detection using individual Au nanoparticles with minimized localized surface plasmon resonance variations. Anal Chem 83(7):2605–2612
We gratefully thank financial support from the Ministry of Education of Singapore (MOE2012-T2-1-058).
Electronic Supplementary Material
Below is the link to the electronic supplementary material.
(DOCX 3,285 kb)
About this article
Cite this article
Wu, B., Chen, LC., Huang, Y. et al. Multiplexed Biomolecular Detection Based on Single Nanoparticles Immobilized on Pneumatically Controlled Microfluidic Chip. Plasmonics 9, 801–807 (2014). https://doi.org/10.1007/s11468-013-9661-4