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Multiplexed Biomolecular Detection Based on Single Nanoparticles Immobilized on Pneumatically Controlled Microfluidic Chip

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Abstract

A microfluidic chip integrated with pneumatically controlled valves was developed for multiplexed biomolecular detection via localized surface plasmonic resonance (LSPR) of single gold nanorod. The cost-effective microfluidic chip was assembled by polydimethylsiloxane layers and glass substrates with a precisely controlled thickness. The thin and flat microfluidic chip fitted the narrow space of dark-field microscopy and enabled the recording of single-nanoparticle LSPR responses. Aptamer-antigen-antibody sandwiched detection scheme was utilized to enhance the LSPR responses for label-free biomolecular detection. This microfluidic chip successfully demonstrated the multiplexed detection of three independent analytes (PSA, IgE, and thrombin).

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Acknowledgments

We gratefully thank financial support from the Ministry of Education of Singapore (MOE2012-T2-1-058).

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Authors and Affiliations

  1. School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, 637457, Singapore

    Bo Wu, Li-Chan Chen, Youju Huang, Yiming Zhang, Yuejun Kang & Dong-Hwan Kim

Authors
  1. Bo Wu
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  2. Li-Chan Chen
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  3. Youju Huang
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  4. Yiming Zhang
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  5. Yuejun Kang
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  6. Dong-Hwan Kim
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Corresponding author

Correspondence to Dong-Hwan Kim.

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Wu, B., Chen, LC., Huang, Y. et al. Multiplexed Biomolecular Detection Based on Single Nanoparticles Immobilized on Pneumatically Controlled Microfluidic Chip. Plasmonics 9, 801–807 (2014). https://doi.org/10.1007/s11468-013-9661-4

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  • DOI: https://doi.org/10.1007/s11468-013-9661-4

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