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A Highly Sensitive Resonance Scattering Assay for Immunoglobulin M Using Ag(I)–Hydroquinone–Immunonanogold Catalytic Reaction

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Abstract

Ten-nanometer nanogold showed the strongest catalytic effect on the particle reaction between Ag(I) and hydroquinone to form nanosilver particles that exhibited the strongest resonance scattering (RS) peak at 350 nm. The enhanced RS intensity was linear to the nanogold concentration in the range of 30–5,700 nM Au. The nanogold was used to label goat antihuman immunoglobulin M (GIgM) to obtain an immunonanogold probe (AuGIgM) for immunoglobulin M (IgM). Based on the nanogold-labeled immunoreaction between IgM and AuGIgM, centrifugation, and AuGIgM–Ag(I)–hydroquinone nanocatalytic reaction, a highly sensitive and selective immunonanogold-catalytic Ag particle RS assay for 0.2–300 ng mL−1 IgM was proposed, with a detection limit of 0.1 ng mL−1. This assay was simple and sensitive and was applied to assay IgM in serum samples, with satisfactory results.

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Acknowledgements

This work was supported by the National Scientific Foundation of China (20667001, 20365001), the Research Funds of the Guangxi Key Laboratory of Environmental Engineering, Protection, and Assessment, and the Foundation of New Century Ten-Hundred-Thousand Talents of Guangxi.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, Guangxi University, Nanning, 530004, China

    Xiaoling Wei

  2. Guangxi Key Laboratory of Environmental Engineering, Protection, and Assessment, Guangxi Normal University, Guilin, 541004, China

    Mingjing Zou, Zhiliang Jiang, Qingye Liu & Guiqing Wen

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  1. Xiaoling Wei
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  2. Mingjing Zou
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  3. Zhiliang Jiang
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  4. Qingye Liu
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  5. Guiqing Wen
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Correspondence to Zhiliang Jiang.

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Wei, X., Zou, M., Jiang, Z. et al. A Highly Sensitive Resonance Scattering Assay for Immunoglobulin M Using Ag(I)–Hydroquinone–Immunonanogold Catalytic Reaction. Plasmonics 3, 73–78 (2008). https://doi.org/10.1007/s11468-008-9060-4

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  • DOI: https://doi.org/10.1007/s11468-008-9060-4

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