Application of surface plasmon resonance imaging technique for iPSCs identification

Abstract

Induced pluripotent stem cells (iPSCs) are an attractive cell source for regenerative medicine through cell therapy or drug screening. But application of iPSCs in regenerative medicine requires rapid and accurate characterization of iPSCs. Here, we demonstrate the detection of multiple antigens present in iPSC lysate using rapid, label-free surface plasmon resonance imaging (SPRi) assay. Validation of pluripotency is an important aspect of iPSC research. In this study, we fabricated antibody array against pluripotency biomarkers and found that our array successfully detect corresponding antigens in stem cell lysate. Each antibody recognized its specific antigens presented in iPSC lysates and a certain degree of variability was observed in comparison with other cell lysates. The results suggested that SPRi is a versatile technology feasible for the detection of multiple antigens presented in iPSC lysate. Further extension of this method may be applied in the characterization and high-throughput biomarker profiling of iPSCs.

摘要

诱导多能干细胞(iPSCs)是再生医学领域,尤其是细胞治疗和药物筛选研究中非常有吸引力的细胞来源。然而,再生医学需要对iPSCs进行快速、精准鉴别。本研究中,我们开发了针对多能性生物标记物的抗体阵列,分别以胚胎干细胞(ESCs)和鼠成纤维细胞(MEF)裂解液分别作为本实验的阳性和阴性对照,应用快速且无需标记的表面等离激元共振成像(SPRi)技术对iPSCs裂解液中的多种抗原进行检测。每一种抗体都与iPSC裂解液中相应的抗原显示出特异性识别。这一结果表明,SPRi技术适合于检测iPSCs裂解液中的多种抗原,并具有通用性,利用SPRi技术可以用于对干细胞裂解液中生物标志物的高通量鉴别分析。