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Detection and analysis of DNA recapture through a solid-state nanopore

  • Article
  • Analytical Chemistry
  • Published:
Chinese Science Bulletin

Abstract

Motion control of a single molecule through a solid-state nanopore offers a new perspective on detecting and analyzing single biomolecules. Repeat recapture of a single DNA molecule reveals the dynamics in DNA translocation through a nanopore and may significantly increase the signal-to-noise ratio for DNA base distinguishing. However, the transient current at the moment of voltage reversal prevents the observation of instantly recaptured molecules and invalidates the continuous DNA ping-pong control. We performed and analyzed the DNA translocation and recapture experiment in a silicon nitride solid-state nanopore. Numerical calculation of molecular motion clearly shows the recapture dynamics with different delay times. The prohibited time when the data acquisition system is saturated by the transient current is derived by equivalent circuit analysis and finite element simulation. The COMSOL simulation reveals that the membrane capacitance plays an important role in determining the electric field distribution during the charging process. As a result of the transient charging process, a non-constant driving force pulls the DNA back to nanopores faster than theoretically predicted. The observed long time constant in the transient current trace is explained by the dielectric absorption of the membrane capacitor.

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Acknowledgments

This work was supported by the National Basic Research Program of China (2012CB933002) and Strategic Priority Research Program (B) of the Chinese Academy of Sciences (XDB07030100). X. Shan acknowledges the financial aid from Open Research Fund Program of the State Key Laboratory of Low-Dimensional Quantum Physics (KF201201) and X. Lu thanks the support of Hundred Talent Program of Chinese Academy of Sciences.

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The authors declare that they have no conflict of interest.

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Correspondence to Xinghua Lu.

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SPECIAL TOPIC: Nanopore Analysis

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Hu, Y., Zhou, Z., Shan, X. et al. Detection and analysis of DNA recapture through a solid-state nanopore. Chin. Sci. Bull. 59, 4953–4959 (2014). https://doi.org/10.1007/s11434-014-0662-2

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  • DOI: https://doi.org/10.1007/s11434-014-0662-2

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