Chinese Science Bulletin

, Volume 58, Issue 36, pp 4593–4601

Mechanism of gold nanoparticle induced simultaneously increased PCR efficiency and specificity

  • Yan Lin
  • Jia Li
  • Jing Yao
  • Yong Liang
  • Jie Zhang
  • QunFang Zhou
  • GuiBin Jiang
Article Environmental Chemistry

DOI: 10.1007/s11434-013-6080-z

Cite this article as:
Lin, Y., Li, J., Yao, J. et al. Chin. Sci. Bull. (2013) 58: 4593. doi:10.1007/s11434-013-6080-z

Abstract

Gold nanoparticles (AuNPs) have been reported to induce faster heat transfer of polymerase chain reaction (PCR) solution to enhance PCR efficiency. AuNPs can also increase the specificity of PCR by functioning analogously to single-stranded DNA binding protein (SSB). However, the simple structure of AuNPs makes it difficult to determine how AuNPs affect PCR efficiency and specificity. This study aimed to elucidate the effect of AuNPs on PCR efficiency by altering the denaturation times and annealing temperatures. In addition, comparative study of the effect of AuNPs on PCR inhibition caused by two competitive primers allowed investigation of the mechanisms of AuNP-enhanced PCR accuracy. Finally, heat-treated salmon sperm DNA was used to evaluate whether AuNPs could eliminate the inhibitory PCR effect caused by DNA impurities. This study demonstrated that enhanced thermal conductivity by AuNPs was the main mechanism for increased PCR efficiency and specificity. AuNPs promoted efficient double-stranded DNA template unwinding and dissociation between mismatched primers, DNA fragments and template, and enhanced PCR efficiency and specificity simultaneously. Thus, this significant finding suggests the future use of AuNP-assisted PCR in different fields, especially in rapid clinical diagnosis and screening by PCR.

Keywords

gold nanoparticles PCR efficiency PCR specificity thermal conductivity single-stranded DNA binding protein 

Copyright information

© The Author(s) 2013

Authors and Affiliations

  • Yan Lin
    • 1
  • Jia Li
    • 2
  • Jing Yao
    • 2
  • Yong Liang
    • 2
    • 3
  • Jie Zhang
    • 1
  • QunFang Zhou
    • 4
  • GuiBin Jiang
    • 4
  1. 1.Key Laboratory of Subtropical Agriculture & Environment of the Ministry of AgricultureHuazhong Agricultural UniversityWuhanChina
  2. 2.School of MedicineJianghan UniversityWuhanChina
  3. 3.Key Laboratory of Optoelectronic Chemical Materials and Devices of Ministry of EducationJianghan UniversityWuhanChina
  4. 4.State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental SciencesChinese Academy of SciencesBeijingChina

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