Abstract
A new strategy is performed to fabricate conjugated polymer microarray with the assistance of protein in this work. The water-soluble cationic conjugated polymer employed in the present work is capable of absorbing light at 510 nm, which makes it compatible with a variety of commercial microarray scanners. It is demonstrated that the protein-assisted conjugated polymer microarray exhibits higher fluorescence signal and better stability in comparison with the case without protein. The conjugated polymer microarray can be used for sensitive detection of picric acid (PA). A major advantage of our approach is its simplicity and chemical linking is not required between the conjugated polymer and microarray substrate. Considering the simplicity of the preparation of the conjugated polymer microarray, it is anticipated that novel sensing platforms will be constructed by employing this versatile method.
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References
Kumar A, Goel G, Fehrencach E, et al. Microarrays: The technology, analysis and application. Eng Life Sci, 2005, 5: 215–222
Heller M J. DNA microarray technology: Devices, systems, and applications. Annu Rev Biomed Eng, 2002, 4: 129–153
Dirk W, Pascal J, Christof N, et al. Applications of protein biochips in biomedical and biotechnological research. Angew Chem Int Ed, 2009, 48: 7744–7751
Sassolas A, Leca-Bouvier B D, Blum L J. DNA biosensors and microarrays. Chem Rev, 2008, 108: 109–139
Yoo S M, Choi J Y, Yun J K, et al. DNA microarray-based identification of bacterial and fungal pathogens in bloodstream infections. Mol Cell Probes, 2010, 24: 44–52
Giljohann D A, Seferos D S, Daniel W L, et al. Gold nanoparticles for biology and medicine. Angew Chem Int Ed, 2010, 49: 3280–3294
Rousserie G, Sukhanova A, Even-Desrumeaux K, et al. Semiconductor quantum dots for multiplexed bio-detection on solid-state microarrays. Crit Rev Oncol Hemat, 2010, 74: 1–15
Thomas S W, Joly G D, Swager T M. Chemical sensors based on amplifying fluorescent conjugated polymers. Chem Rev, 2007, 107: 1339–1386
Duan X R, Liu L B, Wang S, et al. Cationic conjugated polymers for optical detection of DNA methylation lesions and single nucleotide polymorphisms. Acc Chem Res, 2009, 43: 260–270
Zhu C L, Liu L B, Wang S, et al. Water-soluble conjugated polymers for imaging, diagnosis, and therapy. Chem Rev, 2012, 112: 4687–4735
Ho H A, Najari A, Leclerc M. Optical detection of DNA and proteins moth cationic polythiophenes. Acc Chem Res, 2008, 41: 168–178
Jiang H, Taranekar P, Schanze K S, et al. Conjugated polyelectrolytes: Synthesis, photophysics, and applications. Angew Chem Int Ed, 2009, 48: 4300–4316
Pecher J, Mecking S. Nanoparticles of conjugated polymers. Chem Rev, 2010, 110: 6260–6279
Duarte A, Pu K Y, Liu B, et al. Recent advances in conjugated polyelectrolytes for emerging optoelectronic applications. Chem Mater, 2011, 23: 501–515
Lee K, Povlich L K, Kim J. Recent advances in fluorescent and colorimetric conjugated polymer-based biosensors. Analyst, 2010, 135: 2179–2189
Wen Q, Tang H, Wang S, et al. Synthesis and characterization of oligofluorene nanoparticles for cell imaging. Acta Chim Sin, 2012, 70: 2137–2143
An L L, Liu L B, Wang S. Cationic conjugated polymers for homogeneous and sensitive fluorescence detection of hyaluronidase. Sci China Chem, 2009, 52: 827–832
Najari A, Ho H A, Leclerc M, et al. Reagentless ultrasensitive specific DNA array detection based on responsive polymeric biochips. Anal Chem, 2006, 78: 7896–7899
Aberem M B, Najari A, Leclerc M, et al. Protein detecting arrays based on cationic polythiophene-DNA-aptamer complexes. Adv Mater, 2006, 18: 2703–2707
Wigenius J A, Magnusson K, Inganas O, et al. DNA chips with conjugated polyelectrolytes in resonance energy transfer mode. Langmuir, 2009, 26: 3753–3759
Lee K, Rouillard J M, Kim J, et al. Signal-amplifying conjugated polymer-DNA hybrid chips. Angew Chem Int Ed, 2007, 46: 4667–4670
Lee K, Maisel K, Kim J, et al. Sensitive and selective label-free DNA detection by conjugated polymer-based microarrays and intercalating dye. Chem Mater, 2008, 20: 2848–2850
Lee K, Rouillard J M, Kim J, et al. Conjugated polymers combined with a molecular beacon for label-free and self-signal-amplifying DNA microarrays. Adv Funct Mater, 2009, 19: 3317–3325
Lee J, Kim H, Kim J. Polydiacetylene liposome arrays for selective potassium detection. J Am Chem Soc, 2008, 130: 5010–5011
Lee J, Jun H, Kim J. Polydiacetylene-liposome microarrays for selective and sensitive mercury (II) detection. Adv Mater, 2009, 21: 3674–3677
Liu B, Bazan G C. Methods for strand-specific DNA detection with cationic conjugated polymers suitable for incorporation into DNA chips and microarrays. Proc Natl Acad Sci USA, 2005, 102: 589–593
Sun C J, Gaylord B S, Bazan G C, et al. Application of cationic conjugated polymers in microarrays using label-free DNA targets. Nat Protoc, 2007, 2: 2148–2151
Lv F T, Feng X L, Wang S, et al. Development of film sensors based on conjugated polymers for copper (II) ion detection. Adv Funct Mater, 2011, 21: 845–850
He F, Liu L B, Li L D. Water-soluble conjugated polymers for amplified fluorescence detection of template-independent DNA elongation catalyzed by polymerase. Adv Funct Mater, 2011, 21: 3143–3149
Pimienta V, Etchenique R, Buhse T. On the origin of electrochemical oscillations in the picric acid/ctab two-phase system. J Phys Chem A, 2001, 105: 10037–10044
Sohn H, Calhoun R M, Trogler W C. Detection of TNT and picric acid on surfaces and in seawater by using photoluminescent polysiloles. Angew Chem Int Ed, 2001, 40: 2104–2105
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Lü, F., Feng, X., Liu, L. et al. Protein-assisted conjugated polymer microarray: Fabrication and sensing applications. Chin. Sci. Bull. 58, 4039–4044 (2013). https://doi.org/10.1007/s11434-013-6025-6
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DOI: https://doi.org/10.1007/s11434-013-6025-6