Chinese Science Bulletin

, Volume 58, Issue 26, pp 3256–3265 | Cite as

Knockdown of sAC affects sperm hyperactivation by cAMP-signaling pathway in male rat (Rattus norvegicus)

  • Jing Yu
  • Shuai Zhou
  • XiaoQiang Jiang
  • JingYan Bai
  • GenLin Wang
Open Access
Article Developmental Biology

Abstract

Soluble adenylyl cyclase (sAC) plays a critical role in male fertility in mammals by regulating sperm hyperactivation. We aimed to study the mechanism of sAC in this phenomenon and to explore potential target sites for male contraception. In this study, in vivo electroporation and rete testis microinjection-mediated short hairpin (sh)RNA plasmids were adopted to silence sAC gene expression in male rats. The results showed that high transfection efficiency (shRNA717, 49.0% and shRNA4205, 65.0%) was achieved by shRNA plasmids injected directly into the rete testis. When the sAC was downregulated, the cyclic adenosine monophosphate (cAMP) content and protein phosphorylation level of spermatozoa both declined with a significantly lower hyperactivation rate compared with negative controls. The highest transfection efficiency occurred at 15 d and was obviously time dependent. Bioinformatic and experimental results showed that sAC and tmAC both belong to the AC family and might have analogous functions. ShRNA717 and shRNA4205 were the best targets for the sAC gene, suggesting that they could be candidates for male contraception. Thus, it appears feasible to achieve male contraception by silencing the expression of sAC, affecting sperm hyperactivation via a cAMP-mediated signaling pathway.

Keywords

Rattus norvegicus sAC male contraception hyperactivation cAMP shRNA 

Supplementary material

11434_2013_5928_MOESM1_ESM.pdf (384 kb)
Supplementary material, approximately 383 KB.

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Copyright information

© The Author(s) 2013

Authors and Affiliations

  • Jing Yu
    • 1
  • Shuai Zhou
    • 1
  • XiaoQiang Jiang
    • 1
  • JingYan Bai
    • 1
  • GenLin Wang
    • 1
  1. 1.College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingChina

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