Abstract
To investigate the dynamics of phytoplankton size structure in the Pearl River estuary, concentrations of size-fractionated chlorophyll a (Chl a) were determined during four cruises carried out in 2008 and 2010. The distribution of Chl a in this geographical location showed a high degree of temporal variation. Chl a concentrations were highest in autumn, approximately three times higher than those in summer and winter. Microphytoplankton was the dominant contributor, accounting for 66.9% of the Chl a concentration in autumn 2008. In summer and spring 2008, nano-sized cells dominated the phytoplankton population throughout the study region. During the winter cruise, two different areas of water were found, characterized by (1) low salinity and high nutrient content and (2) high salinity and low nutrient content; nano- and picophytoplankton co-dominated the first area, while microphytoplankton dominated the second. It is arguable that grazing could have played a role in determining phytoplankton community size structure in winter. Nutrient concentrations were assumed not to limit phytoplankton growth during the investigation period. Size-differential capacity in competing for the resources available under different hydrodynamic conditions seemed to be the major factor in determining seasonal variation in the structure of the phytoplankton communities. High N:P ratios in the Pearl River estuary had major implications for nutrient pollution control. Our results indicated that studies of phytoplankton size structure provide greater insight into phytoplankton dynamics and are necessary to better manage water quality in the Pearl River estuary.
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Cloern J E, Dufford R. Phytoplankton community ecology: Principles applied in San Francisco Bay. Mar Ecol Prog Ser, 2005, 285: 11–28
Butrón A, Iriarte A, Madariage I. Size-fractionated phytoplankton biomass, primary production and respiration in the Nervión-Ibaizabal estuary: A comparison with other nearshore coastal and estuarine ecosystems from the Bay of Biscay. Cont Shelf Res, 2009, 29: 1088–1102
Fu M Z, Wang Z L, Li Y, et al. Phytoplankton biomass size structure and its regulation in the Southern Yellow Sea (China): Seasonal variability. Cont Shelf Res, 2009, 29: 2178–2194
Malone T C. Algal size. In: Morris I, ed. The Physiological Ecology of Phytoplankton. London: Blackwell Scientific Publications, 1980. 433–465
Platt T, Silver W. Ecology, physiology, allometry and dimensionality. J Theor Biol, 1981, 93: 855–860
Tamigneaux E, Legendre L, Klein B, et al. Seasonal dynamics and potential fate of size-fractionated phytoplankton in a temperate nearshore environment (Western Gulf of St. Lawrence, Canada). Estuar Coast Shelf S, 1999, 48: 253–269
Raven J A. The twelfth Transley Lecture. Small is beautiful: The picophytoplankton. Funct Ecol, 1998, 12: 503–513
Finkel Z V, Irwin A J, Schofield O. Resource limitation alters the 3/4 size scaling of metabolic rates in phytoplankton. Mar Ecol Prog Ser, 2004, 273: 269–279
Cermeño P, Maraón E, Rodríguez J, et al. Large-sized phytoplanktion sustain higher carbon specific photosynthesis than smaller cells in a coastal eutrophic ecosystem. Mar Ecol Prog Ser, 2005, 297: 51–60
Cermeño P, Maraón E, Pérez V, et al. Phytoplankton size structure and primary production in a highly dynamic coastal ecosystem (Ría de Vigo, NW-Spain): Seasonal and short-time scale variability. Estuar Coast Shelf S, 2006, 67: 251–266
Marañón E, Cermeño P, Rodríguez J, et al. Scaling of phytoplankton photosynthesis and cell size in the ocean. Limnol Oceanogr, 2007, 52: 2190–2198
Marañón E. Inter-specific scaling of phytoplankton production and cell size in the field. J Plankton Res, 2008, 30: 157–163
Zhao H T. Evolution of the Pearl River Estuary (in Chinese). Beijing: Ocean Press, 1990
Chen Y T, Luo Z R. Modern sedimentary velocity and their reflected sedimentary characteristics in the Pearl River Mouth (in Chinese). J Trop Oceanol, 1991, 10: 57–64
Huang X P, Huang L M, Yue W Z. The characteristics of nutrients and eutrophication in the Pearl River estuary, South China. Mar Pollut Bull, 2003, 47: 30–36
Tang Y L. The Analysis and Countermeasure of Environmental Problem in China (in Chinese). Guangzhou: Zhongshan University Press, 1997
Wen W Y, Zhang G X, Du W C. A study on water pollution in the Zhujiang (Pearl River) estuary. In: Wong C K, Chu K H, Chen Q C, et al, eds. Environmental Research in Pearl River and Coastal Area (in Chinese). Guangzhou: Guangdong Higher Education Press, 1995. 99–108
Yin K D, Qian P Y, Wu M C S, et al. Shift from P to N limitation of phytoplankton growth across the Pearl River estuarine plume during summer. Mar Ecol Prog Ser, 2001, 221: 17–28
Boudreau P R, Dickie L M, Kerr S R. Body-size spectra of production and biomass as system-level indicators of ecological dynamics. J Theor Biol, 1991, 152: 329–339
Sin Y, Wetzel R L, Anderson I C. Seasonal variations of size fractionated phytoplankton along the salinity gradient in the York River Estuary, Virginia. J Plankton Res, 2000, 22: 1945–1960
Cai Y M, Ning X L, Liu Z L. Studies on primary production and new production of the Zhujiang Estuary, China (in Chinese). Acta Oceanol Sin, 2002, 24: 101–111
Yin K D, Zhang J L, Qian P Y, et al. Effect of wind events on phytoplankton blooms in the Pearl River estuary during summer. Cont Shelf Res, 2004, 24: 1909–1923
Huang B Q, Hong H S, Ke L, et al. Size-fractionated phytoplankton biomass and productivity in the Zhujiang River Estuary in China. Acta Oceanol Sin, 2005, 27: 180–186
Qiu D J, Huang L M, Zhang J L, et al. Phytoplankton dynamics in and near the highly eutrophic Pearl River Estuary, South China Sea. Cont Shelf Res, 2010, 30: 177–186
Grasshoff K, Kremling K, Ehrhardt M. Methods of Seawater Analysis. Weinheim: Wiley-VCH, 1999. 600
Parsons T R, Maita Y, Lalli C M. A Manual of Chemical and Biological Methods for Seawater Analysis. New York: Pergamon Press, 1984. 22–25
Utermöhl H. Zur Vervollkommnung der quantitativen Phytoplankton-Methodik. Mitteilungen der. Internationale Vereinigung für theoretischeund angewandte Limnologie, 1958, 9: 1–38
Buchanan C, Lacouture R V, Marshall H G, et al. Phytoplankton reference communities for Chesapeake Bay and its Tidal Tributaries. Estuar, 2005, 28: 138–159
Lin I, Liu W T, Wu C C, et al. New evidence for enhanced ocean primary production triggered by tropical cyclone. Geophys Res Lett, 2003, 30: 1718
Chang J, Chung C C, Gong G C. Influences of cyclones on chlorophyll a concentration and Synechococcus abundance in a subtropical western Pacific coastal ecosystem. Mar Ecol Prog Ser, 1996, 140: 199–205
Delesalle B, Pichon M, Frankignoulle M, et al. Effects of a cyclone on coral reef phytoplankton biomass, primary production and composition (Moorea Island, French Polynesia). J Plankton Res, 1993, 15: 1413–1423
Kiørboe T. Turbulence, phytoplankton cell size and the structure of pelagic food webs. Adv Mar Biol, 1993, 29: 1–72
Agawin N R S, Duarte C M, Agustí S. Nutrient and temperature control of the contribution of picoplankton to phytoplankton biomass and production. Limnol Oceanogr, 2000, 45: 591–600
Dortch Q, Whitledge T E. Does nitrogen or silicon limit phytoplankton production in the Mississipi River plume and nearby regions? Cont Shelf Res, 1992, 12: 1293–1309
Zhang J, Yu Z G, Wang J T, et al. The subtropical Zhujiang (Pearl River) estuary: Nutrient, trace species and their relationship to photosynthesis. Estuar Coast Shelf S, 1999, 49: 385–400
Harrison P J, Yin K D, Lee J H W, et al. Physical-biological coupling in the Pearl River Estuary. Cont Shelf Res, 2008, 28: 1405–1415
Yin K D, Qian P Y, Chen J C, et al. Dynamics of nutrients and phytoplankton biomass in the Pearl River estuary and adjacent waters of Hong Kong during summer: Preliminary evidence for phosphorus and silicon limitation. Mar Ecol Prog Ser, 2000, 194: 295–305
Murphy L S, Haugen E M. The distribution and abundance of phototrophic ultraplankton in the North Atlantic. Limnol Oceanogr, 1985, 30: 47–58
Marañón E, Berhenfeld M J, González N, et al. High variability of primary production in oligotrophic waters of the Atlantic Ocean: Uncoupling from phytoplankton biomass and size structure. Mar Ecol Prog Ser, 2003, 257: 1–11
Riegman R, Kuipers B R, Noordeloos A A M, et al. Size-differential control of phytoplankton and the structure of plankton communities. Netherlands J Sea Res, 1993, 31: 255–265
Rodríguez J, Tintore J, Allen J T, et al. Mesoscale vertical motion and the size structure of phytoplankton in the ocean. Nature, 2001, 410: 360–363
Wong M H, Cheung K C. China Estuarine Systems: Pearl River Estuary and Mirs Bay. In: Smith, S V, Dupra V, Crossland M, et al, eds. Estuarine Systems of the South China Sea Region: Carbon, Nitrogen and Phosphorus. LOCIZ Reports and Studies No.14, LOICZ, Texel, The Netherlands, 2000. 7–16
Probyn T A. Nitrogen uptake by size-fractionated phytoplankton populations on the southern Benguela upwelling system. Mar Ecol Prog Ser, 1985, 22: 249–258
Sin Y, Wetzel R L, Anderson I C. Seasonal variations of size fractionated phytoplankton along the salinity gradient in the York River Estuary, Virginia. J Plankton Res, 2000, 22: 1945–1960
Banse K. Grazing, temporal changes of phytoplankton concentrations, and the microbial loop in the open sea. In: Falkowski P G, Woodhead A D, eds. Primary Productivity and Biogeochemical Cycles in the Sea. New York: Plenum Press, 1992. 409–440
Uye S. Impact of copepod grazing on the red-tide flagellate Chattonella antiqua. Marine Biol, 1986, 92: 35–43
Bautista B, Harris R P. Copepod gut contents, ingestion rates and grazing impact on phytoplankton in relation to size structure of zooplankton and phytoplankton during a spring bloom. Mar Ecol Prog Ser, 1992, 82: 41–50
Nejstgaard J C, Bamstedt U, Bageoien E, et al. Algal constraints on copepod grazing. Growth state, toxicity, cell size, and season as regulating factors. ICES J Mar Sci, 1995, 52: 347–357
Hansen B W, Hygum B H, Brozek M, et al. Food web interaction in a Calanus finmarchicus dominated pelagic ecosystem-A mesocosm study. J Plankton Res, 2000, 22: 569–588
Tan Y H, Huang L M, Chen Q C, et al. Seasonal variation in zooplankton composition and grazing impact on phytoplankton standing stock in the Pearl River Estuary, China. Cont Shelf Res, 2004, 24: 1949–1968
Li K Z, Yin J Q, Huang L M, et al. Spatial and temporal variations of mesozooplankton in the Pearl River estuary, China. Estuar Coast Shelf S, 2006, 67: 543–552
Li K Z, Yin J Q, Huang L M, et al. Study on planktonic copepods ecology in the Pearl River estuary (in Chinese). Ecol Sci, 2007, 26: 97–102
Fang H D, Zhu A J, Dong Y H, et al. Study on the variations of zooplankton community in the Pearl River Estuary in 2005–2006 (in Chinese). Taiwan Strait, 2009, 28: 30–37
Wong L Y, Chen J X, Xie H, et al. A model study of the circulation in the Pearl River Estuary (PRE) and its adjacent coastal waters: 1. Simulations and comparison with observations. J Geophys Res, 2003, 25: 1–17
Dong Y H, Cai J D, Qian H L. Nutrient Ratios and its Relationship with Phytoplankton in the Pearl River Estuary (in Chinese). Mar Sci Bull, 2009, 18: 3–10
Dai M, Li C H, Jia X P, et al. Ecological characteristics of phytoplankton in coastal area of Pearl River estuary (in Chinese). Chin J Appl Ecol, 2004, 15: 1389–1394
Tilman D. Resource competition between planktonic algae: An experimental and theoretical approach. Ecology, 1977, 58: 338–348
Lewin J C. Silicification. In: Lewin R A, ed. Physiology and Biochemistry of Algae. New York: Academic, 1962. 445–455
Egge J K. Are diatoms poor competitors at low phosphate concentrations? J Marine Syst, 1998, 16: 191–198
Zhou M J, Shen Z L, Yu R C. Responses of a coastal phytoplankton community to increased nutrient input from the Changjiang (Yangtze) River. Cont Shelf Res, 2008, 28: 1483–1489
Li R X, Zhu M Y, Wang Z L, et al. Mesocosm experiment on competition between two HAB species in East China Sea (in Chinese). Chin J Appl Ecol, 2003, 14: 1049–1054
Huang L M, Jian W J, Song X Y, et al. Species diversity and distribution for phytoplankton of the Pearl River estuary during rainy and dry seasons. Mar Pollut Bull, 2004, 49: 588–596
Elliott J A. Is the future blue-green? A review of the current model predictions of how climate change could affect pelagic freshwater cyanobacteria. Water Res, 2012, 46: 1364–1371
Elliott J A. The seasonal sensitivity of Cyanobacteria and other phytoplankton to changes in flushing rate and water temperature. Global Change Biol, 2010, 16: 864–876
Wen W Y, Huang X P, Zhang G X. Discussion on water pollution across multi-regional in the Pearl River estuary. In: Lin J Z, Zhu T, Hu E B, eds. Proceedings of the China Mainland-Hong Kong Symposium on Regional Environmental Impact Assessment (in Chinese). Hong Kong: Open University of Hong Kong Press, 1999. 471–478
Wong G T F, Gong G C, Liu K K, et al. ’Excess nitrate’ in the East China Sea. Estuar Coast Shelf S, 1998, 46: 411–418
Zhang J, Yu Z G, Raabe T, et al. Dynamics of inorganic nutrient species in the Bohai seawaters. J Marine Syst, 2004, 44: 189–212
Jickells T D. Nutrient biogeochemistry of the coastal zone. Science, 1998, 281: 217–222
Smayda T J. Novel and nuisance phytoplankton blooms in the sea: Evidence for a global epidemic. In: Granéli E, Sundström B, Edler B, et al, eds, Toxic Marine Phytoplankton. New York: Elsevier, 1990. 29–40
Humborg C V, Ittekkot E, Cociasu A, et al. Effect of Danube River dam on Black Sea biogeochemistry and ecosystem structure. Nature, 1997, 386: 385–388
Wei H, Sun J, Moll A, et al. Phytoplankton dynamics in the Bohai Sea-observations and modeling. J Marine Syst, 2004, 44: 233–251
Khan S, Haque M M, Arakawa O, et al. Physiological observations on a diatom Skeletonema costatum (Greville) Cleve. Bangladesh J Fisheries Res, 1998, 2: 109–118
Munawar M, Munawar I F, Leppard G G. Early warning assays: An overview of toxicity testing with phytoplankton in the North American Great Lakes. Hydrobiologia, 1989, 188/189: 237–246
Snoeijs P, Busse S, Potapova M. The importance of diatom cell size in community analysis. J Phycol, 2002, 38: 265–272
Nayar S, Goh B P L, Chou L M. Dynamics in the size structure of Skeletonema costatum (Greville) Cleve under conditions of reduced photosynthetically available radiation in a dredged tropical estuary. J Exp Mar Biol Ecol, 2005, 318: 163–182
Sun J, Liu D Y, Zhong H, et al. A Comparison of Three Methods for Studying Phytoplankton Size Fraction (in Chinese). J Ocean Univ Qingdao, 2003, 33: 917–924
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Li, L., Lu, S., Jiang, T. et al. Seasonal variation of size-fractionated phytoplankton in the Pearl River estuary. Chin. Sci. Bull. 58, 2303–2314 (2013). https://doi.org/10.1007/s11434-013-5823-1
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DOI: https://doi.org/10.1007/s11434-013-5823-1