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Cellular prion protein imaging analysis with aptamer-labeled Ru(bpy) 2+3 -doped silica nanoparticles

  • Article
  • Analytical Chemistry
  • Published:
Chinese Science Bulletin

Abstract

A new type of highly selective aptamer-labeled fluorescent silica nanoparticles [Apt-tris(2,2′-bipyridyl)ruthenium(II)@SiO2 NPs] were prepared through the reverse microemulsion method by using prolonged fluorescence lifetime ruthenium complexes of tris(2,2′-bipyridyl)ruthenium(II) (Ru(bpy) 2+3 ) as the source of the fluorescence for cellular prion protein imaging. Investigations showed that the newly prepared Ru(bpy) 2+3 @SiO2 NPs possessed superior advantages of strong fluorescence, low toxicity, and easy surface modification for bioconjugation. Cell imaging experiments indicated that Apt-Ru(bpy) 2+3 @SiO2 NPs had great tendency to human bone marrow neuroblastoma cells (SK-N-SH cells), since they can express large amount of prion protein on the surface of the cell, while in HeLa cells this phenomenon disappeared for the reason that HeLa cells cannot express prion proteins.

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Acknowledgments

This work was supported by the National Basic Research Program of China (2011CB933600).

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Authors and Affiliations

  1. Education Ministry Key Laboratory on Luminescence and Real-Time Analysis, College of Pharmaceutical Sciences, Southwest University, Chongqing, 400715, China

    Wei Wang, Xiao Li Yan, Xiao Xi Yang & Cheng Zhi Huang

  2. College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, China

    Lei Zhan, Fei Leng & Cheng Zhi Huang

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  1. Wei Wang
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  2. Xiao Li Yan
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  3. Lei Zhan
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  4. Fei Leng
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Corresponding author

Correspondence to Cheng Zhi Huang.

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Wang, W., Yan, X.L., Zhan, L. et al. Cellular prion protein imaging analysis with aptamer-labeled Ru(bpy) 2+3 -doped silica nanoparticles. Chin. Sci. Bull. 59, 147–153 (2014). https://doi.org/10.1007/s11434-013-0052-1

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  • DOI: https://doi.org/10.1007/s11434-013-0052-1

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