Skip to main content
SpringerLink
Log in

A high-sensitive detection method for carvone odor by implanted electrodes in rat olfactory bulb

  • Article
  • Biophysics
  • Published:
Chinese Science Bulletin

Abstract

Mammalian olfactory systems have extraordinary ability to sense and identify various trace odorants. Taking advantages of cell culture and micro-fabrication technologies, olfactory cell- or tissue-based biosensor represent a promising platform for in vitro odorant detection. However, in vitro conditions lead to shortened cell/tissue survivals, and the working life of neuron chips is short. The purpose of this study is to develop an in vivo recording and analyzing method for long-term and repeatable detection of odor stimulation. In this study, we implanted penetrating micro-wire array electrode into the olfactory bulb of conscious rats to obtain odor-evoked electrophysiological activities. Then, we investigated the response of ensembles of mitral/tufted cells to stimulation with carvone at a number of concentrations in time and frequency domains. The stable, repeatable odorant responses from up to 16 neural regions could be obtained for at least 3 weeks. Further, we explored the concentration detection sensitivity limitation of developed method, and found the detection low limit of carvone was below 10−10 mol/L. The result demonstrates that the concentration range of in vivo odorant detection method is much wider than in vitro method.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8

Similar content being viewed by others

References

  1. Lledo PM, Gheusi G, Vincent JD (2005) Information processing in the mammalian olfactory system. Physio Rev 85:281–317

    Article  Google Scholar 

  2. Cometto-Muñiz JE, Abraham MH (2008) Human olfactory detection of homologous n-alcohols measured via concentration-response functions. Pharmacolo Biochem Behav 89:279–291. doi:10.1016/j.pbb.2007.12.023

    Article  Google Scholar 

  3. Doty RL (1994) Olfaction and multiple chemical sensitivity. Toxicol Ind Health 10:359–368

    Google Scholar 

  4. Buck L, Axel R (1991) A novel multigene family may encode odorant receptors: a molecular basis for odor recognition. Cell 65:175–187

    Article  Google Scholar 

  5. Ebrahimi FAW, Chess A (1998) The specification of olfactory neurons. Curr Opin Neurobiol 8:453–457

    Article  Google Scholar 

  6. Zou Z, Horowitz LF, Montmayeur JP et al (2001) Genetic tracing reveals a stereotyped sensory map in the olfactory cortex. Nature 414:173–179

    Article  Google Scholar 

  7. Kay LM, Laurent G (1999) Odor-and context-dependent modulation of mitral cell activity in behaving rats. Nat Neurosci 2:1003–1009

    Article  Google Scholar 

  8. Rudolph AS, Reasor J (2001) Cell and tissue based technologies for environmental detection and medical diagnostics. Biosens Bioelectron 16:429–431

    Article  Google Scholar 

  9. Wang P, Liu Q (2009) Cell-based biosensors: principles and applications. Artech House, Boston

    Google Scholar 

  10. Liu Q, Cai H, Xu Y et al (2006) Olfactory cell-based biosensor: a first step towards a neurochip of bioelectronic nose. Biosens Bioelectron 22:318–322

    Article  Google Scholar 

  11. Liu Q, Ye W, Xiao L et al (2010) Extracellular potentials recording in intact olfactory epithelium by microelectrode array for a bioelectronic nose. Biosens Bioelectron 25:2212–2217

    Article  Google Scholar 

  12. Du L, Wu C, Peng H et al (2012) Bioengineered olfactory sensory neuron-based biosensor for specific odorant detection. Biosens Bioelectron 40:401–406

    Article  Google Scholar 

  13. Liu Q, Ye W, Hu N et al (2010) Olfactory receptor cells respond to odors in a tissue and semiconductor hybrid neuron chip. Biosens Bioelectron 26:1672–1678

    Article  Google Scholar 

  14. Liu Q, Hu N, Zhang F et al (2012) Olfactory epithelium biosensor: odor discrimination of receptor neurons from a bio-hybrid sensing system. Biomed Microdevices 14:1055–1061. doi:10.1007/s10544-012-9705-0

    Google Scholar 

  15. Chapin JK, Moxon KA, Markowitz RS et al (1999) Real-time control of a robot arm using simultaneously recorded neurons in the motor cortex. Nat Neurosci 2:664–670

    Article  Google Scholar 

  16. Nicolelis MAL, Dimitrov D, Carmena JM et al (2003) Chronic, multisite, multielectrode recordings in macaque monkeys. Proc Natl Acad Sci USA 100:11041–11046

    Article  Google Scholar 

  17. Lehmkuhle M, Normann R, Maynard E (2003) High-resolution analysis of the spatio-temporal activity patterns in rat olfactory bulb evoked by enantiomer odors. Chem Senses 28:499–508

    Article  Google Scholar 

  18. Rinberg D, Koulakov A, Gelperin A (2006) Sparse odor coding in awake behaving mice. J Neurosci 26:8857–8865

    Article  Google Scholar 

  19. Bhalla US, Bower JM (1997) Multiday recordings from olfactory bulb neurons in awake freely moving rats: spatially and temporally organized variability in odorant response properties. J Comput Neurosci 4:221–256

    Article  Google Scholar 

  20. Davison IG, Katz LC (2007) Sparse and selective odor coding by mitral/tufted neurons in the main olfactory bulb. J Neurosci 27:2091–2101

    Article  Google Scholar 

  21. Schmidt EM (1999) Electrodes for many single neuron recordings. In: Nicolelis MAL (ed) Methods for neural ensemble recordings. CRC, New York, pp 1–24

    Google Scholar 

  22. Tsai ML, Yen CT (2003) A simple method for fabricating horizontal and vertical microwire arrays. J Neurosci Meth 131:107–110

    Article  Google Scholar 

  23. Mori K, Takahashi YK, Igarashi KM et al (2006) Maps of odorant molecular features in the mammalian olfactory bulb. Physiolo Rev 86:409–433

    Article  Google Scholar 

  24. Cang J, Isaacson JS (2003) In vivo whole-cell recording of odor-evoked synaptic transmission in the rat olfactory bulb. J Neurosci 23:4108–4116

    Google Scholar 

  25. Wellis DP, Scott JW, Harrison TA (1989) Discrimination among odorants by single neurons of the rat olfactory bulb. J Neurophysiol 61:1161–1177

    Google Scholar 

  26. Rubin BD, Katz LC (1999) Optical imaging of odorant representations in the mammalian olfactory bulb. Neuron 23:499–511

    Article  Google Scholar 

  27. Fried HU, Fuss SH, Korsching SI (2002) Selective imaging of presynaptic activity in the mouse olfactory bulb shows concentration and structure dependence of odor responses in identified glomeruli. Proc Natl Acad Sci USA 99:3222–3227

    Article  Google Scholar 

  28. Vincis R, Gschwend O, Bhaukaurally K et al (2012) Dense representation of natural odorants in the mouse olfactory bulb. Nat Neurosci 15:537–539

    Article  Google Scholar 

  29. McCulloch M, Jezierski T, Broffman M et al (2006) Diagnostic accuracy of canine scent detection in early-and late-stage lung and breast cancers. Integr Cancer Ther 5:30–39

    Article  Google Scholar 

  30. Ehmann R, Boedeker E, Friedrich U et al (2012) Canine scent detection in the diagnosis of lung cancer: revisiting a puzzling phenomenon. Eur Respir J 39:669–676

    Article  Google Scholar 

  31. Gazit I, Terkel J (2003) Explosives detection by sniffer dogs following strenuous physical activity. Appl Anim Behav Sci 81:149–161

    Article  Google Scholar 

  32. Fjellanger R, Andersen E, McLean IG (2002) A training program for filter-search mine-detection dogs. Int J Comp Psychol 15:278–287

    Google Scholar 

Download references

Acknowledgments

This work was supported by the National Key Basic Research Program of China (2009CB320303) and the National Natural Science Foundation of China (81027003, 60725102).

Author information

Authors and Affiliations

  1. Biosensor National Special Laboratory, Key Laboratory for Biomedical Engineering of Education Ministry, Department of Biomedical Engineering, Zhejiang University, Hangzhou, 310027, China

    Liujing Zhuang, Ning Hu, Feng Tian, Qi Dong, Liang Hu, Rong Li & Ping Wang

Authors
  1. Liujing Zhuang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ning Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Feng Tian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Qi Dong
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Liang Hu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Rong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Ping Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Ping Wang.

About this article

Cite this article

Zhuang, L., Hu, N., Tian, F. et al. A high-sensitive detection method for carvone odor by implanted electrodes in rat olfactory bulb. Chin. Sci. Bull. 59, 29–37 (2014). https://doi.org/10.1007/s11434-013-0044-1

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11434-013-0044-1

Keywords

Search

Navigation