Chinese Science Bulletin

, Volume 58, Issue 18, pp 2113–2118 | Cite as

MSG is involved in sperm gelatinolytic activity in the prawn, Macrobrachium rosenbergii

  • Fan Yang
  • YeQing Qian
  • WenMing Ma
  • Ye Li
  • JinShu Yang
  • WeiJun Yang
Open Access
Article Special Issue Adaptive Evolution and Conservation Ecology of Wild Animals Progress of Projects Supported by NSFC

Abstract

Proteases in male reproductive tract are considered to play key roles in fertilization processes. In contrast to mammals, there are limited data concerning crustacean sperm proteases. We previously identified a novel sperm gelatinase (MSG) from Macrobrachiumrosenbergii that was inhibited by a male reproduction-related Kazal-type protease inhibitor (MRPINK) specifically. In the present study, MSG was found to be distributed on the vas deferens and terminal ampullae by Western blot. Immunohistochemical analyses revealed that MSG was expressed in secretory epithelial cells and sperm distributed mainly at the base zone. RNA interference (RNAi) mediated knock-down of MSG resulted in a marked loss of sperm gelatinolytic activity. Taken together, the results show that MSG is closely linked to and probably involved in the fertilization process.

Keywords

fertilization RNAi sperm protease male reproductive tract 

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Copyright information

© The Author(s) 2013

Authors and Affiliations

  • Fan Yang
    • 1
  • YeQing Qian
    • 1
  • WenMing Ma
    • 2
  • Ye Li
    • 3
  • JinShu Yang
    • 1
  • WeiJun Yang
    • 1
  1. 1.Key Laboratory of Conservation Biology for Endangered Wildlife of Ministry of Education and College of Life SciencesZhejiang UniversityHangzhouChina
  2. 2.Ocean Research Center of ZhoushanZhejiang UniversityZhoushanChina
  3. 3.Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, Faculty of Life Science and BiotechnologyNingbo UniversityNingboChina

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