Abstract
The entire interaction process between anti-mouse immunoglobulin-G (IgG) and mouse IgG with a concentration range from 1 to 0.25 mg/mL has been label-free detected by the oblique incidence reflectivity difference (OIRD) method. We achieved the two-dimensional scanning images of OIRD Re{Δp-Δs} intensities before and after interactions as well as the curves of reaction dynamic processes. The experimental results suggest that OIRD method not only can label-free distinguish the different concentrations of proteins and detect biological microarrays, but also can real-time monitor the kinetic processes of biomolecular interactions.
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Sun, Y., He, L., Dai, J. et al. Label-free and real-time detection of specific binding of IgG proteins by oblique-incidence reflectivity difference method. Chin. Sci. Bull. 57, 2898–2900 (2012). https://doi.org/10.1007/s11434-012-5263-3
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DOI: https://doi.org/10.1007/s11434-012-5263-3