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Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis

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  • Marine Biology
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  • Published: 01 September 2011
  • Volume 56, pages 2916–2921, (2011)
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Chinese Science Bulletin
Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis
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  • AiYou Huang1,2,
  • GuangCe Wang1,
  • LinWen He1,2,
  • JianFeng Niu1 &
  • …
  • BaoYu Zhang1 
  • 920 Accesses

  • 7 Citations

  • Explore all metrics

Abstract

The seaweed Ulva prolifera exists in 2 different states; attached to rocks or free-floating. However, there is little difference between the structures of the 2 states. U. prolifera thalli show significant differences in growth rate, with the attached thalli growing at a normal rate and free-floating thalli growing at a much faster rate. This raised the possibility that the growth of the two states may be regulated differently. miRNAs are important post-transcriptional regulators. In higher plants and animals, miRNAs have been extensively studied but they have been rarely studied in algae. To identify U. prolifera miRNAs and to investigate their possible roles in proliferation, we constructed and sequenced small RNA (sRNA) libraries from U. prolifera. Our results show that U. prolifera has a complex small RNA system that might play important roles in various processes.

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Authors and Affiliations

  1. Institute of Oceanology, the Chinese Academy of Sciences (IOCAS), Qingdao, 266071, China

    AiYou Huang, GuangCe Wang, LinWen He, JianFeng Niu & BaoYu Zhang

  2. Graduate University of the Chinese Academy of Sciences, Beijing, 100049, China

    AiYou Huang & LinWen He

Authors
  1. AiYou Huang
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  2. GuangCe Wang
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  3. LinWen He
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  4. JianFeng Niu
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  5. BaoYu Zhang
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Corresponding author

Correspondence to GuangCe Wang.

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Huang, A., Wang, G., He, L. et al. Characterization of small RNAs from Ulva prolifera by high-throughput sequencing and bioinformatics analysis. Chin. Sci. Bull. 56, 2916–2921 (2011). https://doi.org/10.1007/s11434-011-4678-6

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  • Received: 08 April 2011

  • Accepted: 04 July 2011

  • Published: 01 September 2011

  • Issue Date: September 2011

  • DOI: https://doi.org/10.1007/s11434-011-4678-6

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Keywords

  • Ulva prolifera
  • small RNA
  • microRNA
  • Solexa
  • bioinformatics
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