Abstract
Butanol is a new kind of very potential biofuels. Enzymatic hydrolysis of corn stalk was utilized in this study to produce butanol by Clostridium acetobutylicum CICC 8008. Plackett-Burman (P-B) design and Central Composite Design (CCD) were adopted to screen crucial factors during fermentation as well as the optimization of experimental conditions. The result demonstrated that among the seven factors, namely, Yeast extract, (NH4)2SO4, KH2PO4, MgSO4, FeSO4, CuSO4 and CaCO3, only CaCO3 was selected as the most critical factor. The optimization experiment results for CaCO3 usage, temperature and reaction time by CCD were determined to be 5.04 g/L, 35°C and 70 h, respectively. A corresponding mathematical model was established to predict the fermentation experiment and maximum butanol yield of 6.57 g/L was acquired. The result of verification experiment under the optimum conditions showed that 6.20 g/L was the maximum butanol yield. This demonstrated that statistical method was a powerful tool for the optimization of butanol production from enzymatic hydrolysis of corn stalk.
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References
Ezeji T C, Qureshi N, Karcher P, et al. Production of butanol from corn. In: Minteer S, ed. Alcoholic Fuels. Boca Raton: CRC Press, 2006
Formanek J, Mackie R, Blaschek H P. Enhanced butanol production by Clostridium beijerinckii BA101 grown in semidefined P2 medium containing 6 percent maltodextrin or glucose. Appl Environ Microbiol, 1997, 63: 2306–2310
Parekh M, Formanek J, Blaschek H P. Development of a cost-effective glucose-corn steep medium for the production of butanol by Clostridium beijerinckii. J Ind Microbiol Biotechnol, 1998, 21: 187–191
Parekh M, Formanek J, Blaschek H P. Pilot-scale production of butanol by Clostridium beijerinckii BA101 using low-cost fermentation medium based on corn steep water. Appl Microbiol Biotechnol, 1999, 51: 152–157
Ladisch M R. Fermentation derived butanol and scenarios for its uses in energy-related applications. Enzyme Microbial Technol, 1991, 13: 280–283
Qureshi N, Maddox I S. Application of novel technology to the ABE fermentation process: An economic analysis. Appl Biochem Biotechnol, 1992, 34: 441–448
Qureshi N, Blaschek H P. Evaluation of recent advances in butanol fermentation, upstream, and downstream processing. Bioproc Biosys Eng, 2001, 24: 219–226
Jones D T, Woods D R. Acetone-butanol fermentation revisited. Microbiol Rev, 1986, 50: 484–524
Ezeji T C, Qureshi N, Blaschek H P. Bioproduction of butanol from biomass: From genes to bioreactors. Curr Opin Biotechnol, 2007, 18: 220–227
Ezeji, T C, Qureshi N, Blaschek H P. Butanol production from agricultural residues: Impact of degradation products on Clostridium beijerinckii growth and butanol fermentation. Biotechnol Bioeng, 2007, 97: 1460–1469
Tabka M G, Herpoël-Gimbert I, Monod F, et al. Enzymatic saccharification of wheat straw for bioethanolproduction by a combined cellulose xylanase and feruloyl esterase treatment. Enzyme Microb Technol, 2006, 39: 897–902
Esteghlalian A, Hashimoto A G, Fenske J J, et al. Modeling and optimization of the dilute-sulfuric-acid pretreatment of corn stover, poplar and switchgrass. Bioresour Technol, 1997, 59: 129–136
Qureshi N, Saha B, Cotta M. Butanol production from wheat straw hydrolysate using Clostridium Beijerinckii. Bioproc Biosys Eng, 2007, 30: 419–427
Madihah M S, Ariff A B, Sahaid K M, et al. Direct fermentation of gelatinized sago starch to acetone-butanol-ethanol by Clostridium acetobutylicum. World J Microbiol Biotechnol, 2001, 17: 567–576
Lai M C, Traxler R W. A coupled two-stage continuous fermentation for solvent production by Clostridium acetobutylicum. Enzyme Microb Technol, 1994, 16: 1021–1025
Guo W Q, Ren N Q, Wang W S, et al. Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology. Bioresour Technol, 2009, 100: 1193–1196
Chung K T, Bryant M P, Robert E. Hungate: Pioneer of anaerobic microbial ecology. Anaerobe, 1997, 3: 213–217
Yukihiro T, Katsuhisa T, Genta K, et al. High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-Stat continuous butyric acid and glucose feeding method. J Biosci Bioeng, 2004, 98: 263–269
Wang Q H, Wang X Q, Ma H Z. Glucoamylase production from food waste by Aspergillus niger under submerged fermentation. Process Biochem, 2008, 43: 280–286
Gupta N, Sahai V, Gupta R. Alkaline lipase from a novel strain Burkholderia multivorans: Statistical medium optimization and production in a bioreactor. Process Biochem, 2007, 42: 518–526
Wang J L, Wei W. Optimization of fermentative hydrogen production process using genetic algorithm based on neural network and response surface methodology. Int J Hydrogen Energy, 2008, 34: 255–261
Gangadharan D, Sivaramakrishnan S, Nampoothiri K M, et al. Response surface methodology for the optimization of alpha amylase production by Bacillus amyloliquefaciens. Bioresour Technol, 2008, 99: 4597–4602
Eevera T, Rajendran K, Saradha S. Biodiesel production process optimization and characterization to assess the suitability of the product for varied environmental conditions. Renewable Energy, 2008, 34: 762–765
Loukas Y L. A Plackett-Burnam screening design directs the efficient formulation of multicomponent DRV liposomes. J Pharm Biomed Anal, 2001, 26: 255–263
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Lin, Y., Wang, J., Wang, X. et al. Optimization of butanol production from corn straw hydrolysate by Clostridium acetobutylicum using response surface method. Chin. Sci. Bull. 56, 1422–1428 (2011). https://doi.org/10.1007/s11434-010-4186-0
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DOI: https://doi.org/10.1007/s11434-010-4186-0