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Chinese Science Bulletin

, Volume 55, Issue 35, pp 4032–4036 | Cite as

Acute and chronic un-ionized ammonia toxicity to ‘all-fish’ growth hormone transgenic common carp (Cyprinus carpio L.)

  • Bo Guan
  • Wei HuEmail author
  • TangLin Zhang
  • Ming Duan
  • DeLiang Li
  • YaPing Wang
  • ZuoYan Zhu
Article Hydrobiology

Abstract

Ammonia is toxic to fish in natural and artificial waters. We evaluated the acute (96 h) and chronic (21 d) toxicity of un-ionized ammonia to GH transgenic common carp (Cyprinus carpio L.) and non-transgenic common carp using a static-renewal bioassay. The 24, 48, 72 and 96 h median lethal concentrations (LC50) of un-ionized ammonia were slightly lower in transgenic carp (2.64, 2.44, 2.28 and 2.16 mg N/L, respectively) than in non-transgenic carp (2.70, 2.64, 2.52 and 2.33 mg N/L, respectively). Similarly, the median lethal time (LT50) was significantly shorter for transgenic carp (1.41, 7.91 and 117.42 h) than for non-transgenic common carp (2.53, 14.06 and 150.44 h) following exposure to 3.86, 3.29, or 2.09 mg N/L, respectively. Moreover, the mortality of transgenic carp was significantly higher than that of non-transgenic carp at all un-ionized ammonia concentrations ((0.91 ± 0.12), (0.48 ± 0.06) and (0.12 ± 0.01) mg N/L) during the 21 d chronic toxicity test. Our results suggest that GH transgenic carp are less tolerant of un-ionized ammonia than non-transgenic carp. Our data are useful for evaluating potential environmental risk, optimizing stocking density in intensive aquaculture and establishing water quality criteria for ammonia in aquaculture.

Keywords

transgenic common carp growth hormone un-ionized ammonia toxicity mortality 

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Copyright information

© Science China Press and Springer-Verlag Berlin Heidelberg 2010

Authors and Affiliations

  • Bo Guan
    • 1
    • 2
  • Wei Hu
    • 1
    Email author
  • TangLin Zhang
    • 1
  • Ming Duan
    • 1
    • 2
  • DeLiang Li
    • 1
    • 2
  • YaPing Wang
    • 1
  • ZuoYan Zhu
    • 1
  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of HydrobiologyChinese Academy of SciencesWuhanChina
  2. 2.Graduate School of Chinese Academy of SciencesBeijingChina

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