Abstract
Osteoblasts were cultured on porous scaffolds of poly(L-lactide-co-glycolide) (PLGA) and PLGA/β-tricalcium phosphate (β-TCP) to evaluate their cytocompatibility. The proliferation of the cells on both scaffolds was examined before and after in vitro degradation for 4, 8 and 12 weeks under static (shaking water bath) and dynamic (cyclic loading) conditions. Results indicate that porous PLGA and PLGA/β-TCP scaffolds have good biocompatibility and can be used as effective templates for guiding the growth of osteoblasts. The degradation of the scaffolds affects the proliferation of osteoblasts and the cell viability decreased with the degradation time.
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Betz R R. Limitations of autograft and allograft: New synthetic solutions. Orthopedics, 2002, 25: 561–570
Shen H, Hu X X, Yang F, et al. An injectable scaffold: rhBMP-2-loaded poly(lactide-co-glycolide)/hydroxyapatite composite micro-spheres. Acta Biomater, 2010, 6: 455–465
Ozkan S, Kalyon D M, Yu X J. Functionally graded beta-TCP/PCL nanocomposite scaffolds: In vitro evaluation with human fetal osteoblast cells for bone tissue engineering. J Biomed Mater Res, 2010, 92A: 1007–1018
Kuo Y C, Yeh C F, Yang J T. Differentiation of bone marrow stromal cells in poly(lactide-co-glycolide)/chitosan scaffolds. Biomaterials, 2009, 30: 6604–6613
Yang Y F, Zhao J, Zhao Y H, et al. Formation of porous PLGA scaffolds by a combining method of thermally induced phase separation and porogen leaching. J Appl Polym Sci, 2008, 109: 1232–1241
Yang Y F, Tang G W, Zhao Y H, et al. Effect of cyclic loading on in vitro degradation of poly(L-lactide-co-glycolide) scaffolds. J Biomater Sci Polym Ed, 2010, 21: 53–66
Yang Y F, Tang G W, Zhao Y H, et al. In vitro degradation of porous poly(L-lactide-co-glycolide)/β-tricalcium phosphate (PLGA/β-TCP) scaffolds under dynamic and static conditions. Polym Degrad Stab, 2008, 93: 1838–1845
Wu L B, Ding J D. In vitro degradation of three-dimensional porous poly(D,L-lactide-co-glycolide) scaffolds for tissue engineering. Biomaterials, 2004, 25: 5821–5830
Wu L B, Ding J D. Effects of porosity and pore size on in vitro degradation of three-dimensional porous poly(D,L-lactide-co-glycolide) scaffolds for tissue engineering. J Biomed Mater Res Part A, 2005, 75A: 767–777
Rohanizadeh R, Swain M V, Mason R S. Gelatin sponges (Gelfoam ®) as a scaffold for osteoblasts. J Mater Sci-Mater Med, 2008, 19: 1173–1182
Yu T, Liu Y, Wang Y, Jing X B, et al. Preparation and bioactivity of the composite of PLGA and hydroxyapatite nanocrystals surface-grafted with L-lactic acid oligomer. Chem J Chinese Univ, 2009, 30: 1439–1444
Qu X, Cui W J, Yang F, et al. The effect of oxygen plasma pretreatment and incubation in modified simulated body fluids on the formation of bone-like apatite on poly (lactide-co-glycolide) (70/30). Biomaterials, 2007, 28: 9–18
Kim H, Kim H E, Salih V, et al. Dissolution control and cellular responses of calcium phosphate coatings on zirconia porous scaffold. J Biomed Mater Res, 2004, 68A: 522–530
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Yang, Y., Tang, G., Zhao, Y. et al. Effect of degradation of PLGA and PLGA/β-TCP scaffolds on the growth of osteoblasts. Chin. Sci. Bull. 56, 982–986 (2011). https://doi.org/10.1007/s11434-010-4132-1
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DOI: https://doi.org/10.1007/s11434-010-4132-1