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1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing

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  • Preclinical Medicine
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Chinese Science Bulletin

Abstract

1A6/DRIM has been identified as UTP20, a small subunit processome component, functioning in 18S rRNA processing. In the present study, the maturation of 28S rRNA and 5.8S rRNA was inhibited when 1A6/DRIM was silenced in HeLa cells; and coincidently, an accumulation of 32S rRNA precursor was observed. Immunoprecipitation was performed with the anti-1A6/DRIM antibody, followed by Northern blot with the ITS2 probe. The results showed that 1A6/DRIM was associated with both 32S and 12S rRNA precursors in vivo. The expression profile of 1A6/DRIM during rRNA processing was investigated by sucrose density gradient fractionation in combination with Western blot analysis. The results demonstrated that 1A6/DRIM was involved in the pre-60S particles in addition to the pre-40S particles and co-sediment with the 32S and 12S rRNA precursors in the nucleolus. Furthermore, the interaction of U8 snoRNA with 1A6/DRIM was revealed by immunoprecipitation. These results demonstrated that 1A6/DRIM interacted with both 32S rRNA and U8 snoRNA, being involved in 28S rRNA and 5.8S rRNA processing.

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Authors and Affiliations

  1. Key Laboratory of Carcinogenesis and Translational Research (Ministry of Education), Genetics Laboratory, Peking University School of Oncology, Beijing Cancer Hospital & Institute, Beijing, 100142, China

    RuiRui Kong, Tao Ning & Yang Ke

  2. Cancer Research Center, Peking University Health Science Center, Beijing, 100191, China

    RuiRui Kong, Wei Han, XiaoJuan Du & Yang Ke

  3. Division Pharma, Roche Diagnostics GmbH, 82372, Penzberg, Germany

    H. Weidle Ulrich

  4. Department of Cell Biology, Peking University Health Science Center, Beijing, 100191, China

    XiaoJuan Du

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  1. RuiRui Kong
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  2. Wei Han
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  3. H. Weidle Ulrich
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  4. Tao Ning
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  5. XiaoJuan Du
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  6. Yang Ke
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Correspondence to XiaoJuan Du or Yang Ke.

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Kong, R., Han, W., Ulrich, H.W. et al. 1A6/DRIM, the human UTP20 functions in 28S and 5.8S rRNA processing. Chin. Sci. Bull. 55, 1770–1776 (2010). https://doi.org/10.1007/s11434-010-3166-8

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  • DOI: https://doi.org/10.1007/s11434-010-3166-8

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