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A drainage-enhancing device for foam fractionation of proteins

  • Articles
  • Bioengineering
  • Published:
Chinese Science Bulletin

Abstract

The foam fractionation of nisin from its fermentation broth was studied. Two types of devices consisting of a rubber piston and a foam riser were developed to enhance foam drainage. The separation performance of these two devices was investigated. Experimental results indicated that the second device could significantly reduce the liquid fraction of the foam leaving the column, ɛout, leading to a higher enrichment of the out-flow stream. As its mounting height increased from 0 to 15 cm, ɛout declined from 7.07‰ to 6.13 ‰ and the maximum nisin activity in the foamate could reach 39.6 IU/μL. The slight increase in nisin inactivation rate indicated the applicability of this method in the recovery and concentration of proteins. Finally, the mechanism of the process was primarily explained by invoking recent work on pneumatic foams. This research provides a basis for the design of multistage draining foam fractionator which could potentially be an effective separation equipment.

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References

  1. Lemlich R. Adsorptive bubble separation methods. Ind Eng Chem, 1968, 60: 16–29

    Article  Google Scholar 

  2. Wungrattanasopon P, Scamehorn J F, Chavedej S, et al. Use of foam flotation to remove tert-Butylphenol from water. Sep Sci Technol, 1996, 31: 1523–1540

    Article  Google Scholar 

  3. Mathews A, Bishnoi P R, Svrcek W Y. Treatment of oil contaminated waste waters by foam fractionation. Water Res, 1979, 13: 385–391

    Article  Google Scholar 

  4. Maruyama H, Seki H, Suzuki A, et al. Batch foam separation of a soluble protein. Water Res, 2007, 41: 710–718

    Article  Google Scholar 

  5. Aksay S, Mazza G. Optimization of protein recovery by foam separation using response surface methodology. J Food Eng, 2007, 79: 598–606

    Article  Google Scholar 

  6. Linke D, Zorn H, Gerken B, et al. Laccase isolation by foam fractionation-New prospects of an old process. Enzyme Microb Technol, 2007, 40: 273–277

    Article  Google Scholar 

  7. Gerken B M, Nicolai A, Linke D, et al. Effective enrichment and recovery of laccase C using continuous foam fractionation. Sep Purif Technol, 2006, 49: 291–294

    Article  Google Scholar 

  8. Lockwood C E, Bummer P M, Jay M. Purification of proteins using foam fractionation. Pharm Res, 1997, 14: 1511–1515

    Article  Google Scholar 

  9. Kruglyakov P M, Karakashev S I, Nguyen A V, et al. Foam drainage. Curr Opin Colloid Interface Sci, 2008, 13: 163–170

    Article  Google Scholar 

  10. Prakash A, Briens C L. Porous gas distributors in bubble columns. Effect of liquid presence on distributor pressure drop. Effect of start-up procedure on distributor performance. Can J Chem Eng, 1990, 68: 204–210

    Article  Google Scholar 

  11. Andou S, Yamagiwa K, Ohkawa A. Effect of gas sparger type on operational characteristics of a bubble column under mechanical foam control. J Chem Technol Biotechnol, 1999, 66: 65–71

    Article  Google Scholar 

  12. Boonyasuwat S, Chavadej S, Malakul P, et al. Anionic and cationic surfactant recovery from water using a multistage foam fractionator. Chem Eng J, 2003, 93: 241–252

    Article  Google Scholar 

  13. Bando Y, Kuze T, Sugimoto T, et al. Development of bubble column for foam separation. Korean J Chem Eng, 2000, 17: 597–599

    Article  Google Scholar 

  14. Ito Y. Method for continuous countercurrent foam separation. 1986

  15. Darton R C, Supino S, Sweeting K J. Development of a multistaged foam fractionation column. Chem Eng Process, 2003, 43: 477–482

    Article  Google Scholar 

  16. Leonard R A, Blacykl J D. Multistage bubble fractionator. Ind Eng Chem, 1978, 17: 358–361

    Google Scholar 

  17. Morgan G. Single and multistage foam fractionation of rinse water with alkyl ethoxylate surfactants. Sep Sci Technol, 2001, 36: 2247–2263

    Article  Google Scholar 

  18. Kruglyakov P M, Khaskova T N. Adsorption accumulation of proteins and dyes in foams of solutions and waste water. Colloids Surf Physicochem Eng Aspects, 2005, 263: 400–404

    Article  Google Scholar 

  19. Hidenori T, Rie H, Kazuaki Y, et al. Effect of perforated plate on concentration of poly(vinyl alcohol) by foam fractionation with external reflux. J Chem Eng Jpn, 2003, 36: 1107–1110

    Article  Google Scholar 

  20. Linkea D, Zorna H, Gerkenb B, et al. Foam fractionation of exo-lipases from a growing fungus (pleurotus sapidus). Lipids, 2005, 40: 323–327

    Article  Google Scholar 

  21. Maruyama H, Seki H, Suzuki A, et al. Variation of saturated surface density of ovalbumin on bubble surface in continuous foam separation. J Colloid Interface Sci, 2006, 299: 416–420

    Article  Google Scholar 

  22. Koehler S A, Hilgenfeldt S, Stone H A. A generalized view of foam drainage: Experiment and theory. Langmuir, 2000, 16: 6327–6341

    Article  Google Scholar 

  23. Sun Q C, Huang J, Wang G. Foam drainage wave coalescing and its energy evolution. Chinese Sci Bull, 2008, 53: 3138–3141

    Article  Google Scholar 

  24. Sun Q C, Ge W, Huang J. Influence of gravity on narrow input forced drainage in 2D liquid foams. Chinese Sci Bull, 2007, 52: 423–427

    Article  Google Scholar 

  25. Bhakta A, Ruckenstein E. Drainage of a standing foam. Langmuir, 1995, 11: 1486–1492

    Article  Google Scholar 

  26. Carolissen-Mackay V, Arendse G, Hastings J W. Purification of bacteriocins of lactic acid bacteria: Problems and pointers. Int J Food Microbiol, 1997, 34: 1–16

    Article  Google Scholar 

  27. Arnaud S J. Physical chemistry in foam drainage and coarsening. J Mater Chem, 2006, 2: 836–849

    Google Scholar 

  28. Clarkson J R, Cui Z F, Darton R C. Protein denaturation in foam I. Mechanism study. J Colloid Interface Sci, 1999, 215: 323–332

    Article  Google Scholar 

  29. Clarkson J R, Cui Z F, Darton R C. Protein denaturation in foam II. Surface activity and conformational change. J Colloid Interface Sci, 1999, 215: 333–338

    Article  Google Scholar 

  30. Liu Z, Liu Z, Wang D, et al. On the denaturation of enzymes in the process of foam fractionation. Bioseparation, 1998, 7: 167–174

    Article  Google Scholar 

  31. Stevenson P. Hydrodynamic theory of rising foam. Miner Eng, 2007, 20: 282–289

    Article  Google Scholar 

  32. Wu Z, Hu G, Yu G. Isolation of Nisin preparation method. PRC, CN 1743339A, 2006-3–8

  33. Wu Z, Wang L, Jing Y, et al. Variable volume fed-batch fermenta tion for nisin production by Lactococcus lactis subsp. lactis W28. Appl Biochem Biotechnol, 2009, 152: 372–382

    Article  Google Scholar 

  34. Neely B C, Eiamwat J, Du L, et al. Modeling a batch foam fractionation process. Bio Bratislava, 2001, 56: 583–589

    Google Scholar 

  35. Webb S D, Page R C, Jay M, et al. Characterization and validation of the gamma-scintigrqaphic method for determining liquid holdup in foam. Appl Radiat Isot, 2002, 57: 243–255

    Article  Google Scholar 

  36. Stevenson P, Mantle M D, Hicks J M. NMRI studies of the free drainage of egg while and meringue mixture froths. Food Hydrocoll, 2007, 21: 221–229

    Article  Google Scholar 

  37. Stevenson P, Stevanov C, Jameson G J. Liquid overflow from a column of rising aqueous froth. Miner Eng, 2003, 16: 1045–1053

    Article  Google Scholar 

  38. Maruyama H, Suzuki A, Seki H, et al. Enrichment in axial direction of aqueous foam in continuous foam separation. Biochem Eng J, 2006, 30: 253–259

    Article  Google Scholar 

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Authors and Affiliations

  1. Department of Bioengineering, Hebei University of Technology, Tianjin, 300130, China

    ZhaoLiang Wu, ShaoYu Qian, HuiJie Zheng & YanLi Zhao

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  1. ZhaoLiang Wu
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  2. ShaoYu Qian
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  3. HuiJie Zheng
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  4. YanLi Zhao
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Correspondence to ZhaoLiang Wu.

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Wu, Z., Qian, S., Zheng, H. et al. A drainage-enhancing device for foam fractionation of proteins. Chin. Sci. Bull. 55, 1213–1220 (2010). https://doi.org/10.1007/s11434-010-0110-x

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  • DOI: https://doi.org/10.1007/s11434-010-0110-x

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