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The effects of gold nanoparticles on the proliferation, differentiation, and mineralization function of MC3T3-E1 cells in vitro

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  • Chemical Biology
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Chinese Science Bulletin

Abstract

This study has investigated the effects of gold nanoparticles (Au NPs) on the proliferation, differentiation, and mineralization of a murine preosteoblast cell line MC3T3-E1 in vitro. The results show that Au NPs with diameters of both 20 and 40 nm promoted the proliferation, differentiation, and mineralization of MC3T3-E1 cells in a time- and dose-dependent manner at the concentrations of 1.5×10−5, 3.0×10−5, and 1.5×10−4 μmol/L. The reverse transcriptase polymerase chain reaction (RT-PCR) indicates that the expressions of runt-related transcription factor 2 (Runx2), bone morphogenetic protein 2 (BMP-2), alkaline phosphatase (ALP), and osteocalcin (OCN) genes increased after the 20 and 40 nm Au NP treatments, and the expression levels were higher than those of the NaF group. The above results suggest that Au NPs have the potential to promote the osteogenic differentiation and mineralization of MC3T3-E1 cells and the particle size plays a significant role in the process. Runx2, BMP-2, ALP, and OCN genes may interact with each other, further stimulating the osteogenic differentiation of MC3T3-E1 cells.

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References

  1. Mazzola L. Commercializing nanotechnology. Nat Biotechnol, 2003, 21: 1137–1143

    Article  Google Scholar 

  2. Paull R, Wolfe J, Hebert P, et al. Investing in nanotechnology. Nat Biotechnol, 2003, 21: 1144–1147

    Article  Google Scholar 

  3. Marie-Christine D, Didier A. Gold nanoparticles: Assembly, supra-molecular chemistry, quantum-size-related properties, and applications toward biology, catalysis, and nanotechnology. Chem Rev, 2004, 104: 293–346

    Article  Google Scholar 

  4. Frederix F, Friedt J M, Choi K H, et al. Biosensing based on light absorption of nanoscaled gold and silver particles. Anal Chem, 2003, 75: 6894–6900

    Article  Google Scholar 

  5. Liu T, Tang J A, Zhao H Q, et al. Particle size effect of the DNA sensor amplified with gold nanoparticles. Langmuir, 2002, 18: 5624–5626

    Article  Google Scholar 

  6. Paciotti G F, Myer L, Weinreich D, et al. Colloidal gold: A novel nanoparticle vector for tumor directed drug delivery. Drug Delivery, 2004, 11: 169–183

    Article  Google Scholar 

  7. Yang N, Sun W H. Gene gun and other non-viral approaches for cancer gene therapy. Nat Med, 1995, 1: 481–483

    Article  Google Scholar 

  8. Luther W. 2nd ed: Industrial application of nanomaterials — chances and risks. Future Technologies, 2004, 54: 1–112

    Google Scholar 

  9. Siegrist M, Wiek A, Helland A, et al. Risks and nanotechnology: The public is more concerned than experts and industry. Nat Nanotech, 2007, 2: 67–68

    Article  Google Scholar 

  10. De Jong W H, Hagens W I, Krystek P, et al. Particle size-dependent organ distribution of gold nanoparticles after intravenous administration. Biomaterials, 2008, 29: 1912–1919

    Article  Google Scholar 

  11. Grislain L, Couvreur P, Lenaerts V, et al. Pharmacokinetics and distribution of a biodegradable drug-carrier. Int J Pharm, 1983, 15: 335–345

    Article  Google Scholar 

  12. Pittenger M F, Mackay A M, Beck S C, et al. Multilineage potential of adult human mesenchymal stem cell. Science, 1999, 284: 143–147

    Article  Google Scholar 

  13. Karsenty G, Wagner E F. Reaching a genetic and molecular understanding of skeletal development. Dev Cell, 2002, 2: 389–406

    Article  Google Scholar 

  14. Kaplan F S, Hayes W C, Keaveny T M, et al. In: Simon S R, ed. Orthopedic Basic Science. Rosemont: American Academy of Orthopaedic Surgeons, 1994. 127–185

    Google Scholar 

  15. Carmichael J, Degraff W G, Gazdar A F, et al. Evaluation of a tetrazolium-based semiautomated colorimetric assay: Assessment of chemosensitivity testing. Cancer Res, 1987, 47: 936–942

    Google Scholar 

  16. Zhao Y, Zou B, Shi Z Y, et al. The effect of 3-hydroxybutyrate on the in vitro differentiation of murine osteoblast MC3T3-E1 and in vivo bone formation in ovariectomized rats. Biomaterials, 2007, 28: 3063–3073

    Article  Google Scholar 

  17. Wang D, Christensen K, Chawla K, et al. Isolation and characterization of MC3T3-E1 preosteoblast subclones with distinct in vitro and in vivo differentiation/mineralization potential. Bone Miner Res, 1999, 14: 893–903

    Article  Google Scholar 

  18. Gori F, Divieti P, Demay M. Cloning and characterization of a novel WD-40 repeat protein that dramatically accelerates osteoblastic differentiation. J Biol Chem, 2001, 276: 46515–46522

    Article  Google Scholar 

  19. Obrant K J, Ivaska K K, Gerdhem P, et al. Biochemical markers of bone turnover are influenced by recently sustained fracture. Bone, 2005, 36: 786–792

    Article  Google Scholar 

  20. Veitch S W, Findlay S C, Hamer A J, et al. Changes in bone mass and bone turnover following tibial shaft fracture. Osteoporos Int, 2006, 17: 364–372

    Article  Google Scholar 

  21. Stein G S, Lian J B. Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr Rev, 1993, 14: 424–442

    Google Scholar 

  22. Li H Z, Zhou Y, Guo G N. Effects of core binding facor α1 on promotion of osteoblastic differentiation from marrow mesenchymal stem cells. Zhongguo Xiufu Chongjian Waike Zazhi (in Chinese), 2006, 20: 121–124

    Google Scholar 

  23. Collin P, Nefussi J R, Wetterwald A, et al. Expression of collagen, osteocalcin, and bone alkaline phosphatase in a mineralizing rat osteoblastic cell culture. Calcif Tissue Int, 1992, 50: 175–183

    Article  Google Scholar 

  24. Lazary A, Balla B, Kosa J P, et al. Effect of gypsum on proliferation and differentiation of MC3T3-E1 mouse osteoblastic cells. Biomaterials, 2007, 28: 393–399

    Article  Google Scholar 

  25. Lipski A M, Pino C J, Haselton F R, et al. The effect of silica nanoparticle-modified surfaces on cell morphology, cytoskeletal organization and function. Biomaterials, 2008, 29: 3836–3846

    Article  Google Scholar 

  26. Roux C, Cha F, Ollivier N, et al. Ti-Cp functionalization by deposition of organic/inorganic silica nanoparticles. Biomol Eng, 2007, 24: 549–554

    Article  Google Scholar 

  27. Guehennec L L, Lopez-Heredia M A, Enkel B, et al. Osteoblastic cell behaviour on different titanium implant surfaces. Acta Biomaterialia, 2008, 4: 535–543

    Article  Google Scholar 

  28. Zhang J C, Li X X, Xu S J, et al. Effect of the rare earth ions on proliferation, differentiation and function of osteoblasts in vitro. Prog Nat Sci (in Chinese), 2004, 14: 404–409

    Google Scholar 

  29. Taton T A. Nanotechnology: Boning up on biology. Nature, 2001, 412: 491–492

    Article  Google Scholar 

  30. Ducy P, Zhang R, Geoffroy V, et al. Osf2/Cbfa1: A transcriptional activator of osteoblast differentiation. Cell, 1997, 89: 747–754

    Article  Google Scholar 

  31. Nakashima K, Zhou X, Kunkel G, et al. The novel zinc finger-containing transcription factor osterix is required for osteoblast differentiation and bone formation. Cell, 2002, 108: 17–29

    Article  Google Scholar 

  32. Yang S Y, Wei D Y, Wang D, et al. In vitro and in vivo synergistic interactions between the Runx2/Cbfa1 transcription factor and bone morphogenetic protein-2 in stimulating osteoblast differentiation. J Bone Miner Res, 2003, 18: 705–715

    Article  Google Scholar 

  33. Beertsen W, Van Den Bos T. Alkaline phosphatase induces the deposition of calcified layers in relation to dentin: An in vitro study to mimic the formation of afibrillar acellular cementum. J Dent Res, 1991, 70: 176–181

    Google Scholar 

  34. Chou Y F, Dunn J C Y, Wu B M. In vitro response of MC3T3-E1 preosteoblasts within three-dimensional apatite-coated PLGA scaffolds. J Biomedic Mater Res, 2005, 75B: 81–90

    Article  Google Scholar 

  35. Komori T, Yagi H, Nomura S, et al. Targeted disruption of Cbfa1 results in a complete lack of bone formation owing to maturational arrest of osteoblasts. Cell, 1997, 89: 755–764

    Article  Google Scholar 

  36. Lee M H, Kim Y J, Kim H J, et al. BMP-2-induced Runx2 expression is mediated by Dlx5, and TGF-β1 opposes the BMP-2-induced osteoblast differentiation by suppression of Dlx5 expression. J Biol Chem, 2003, 278: 34387–34394

    Article  Google Scholar 

  37. Pioletti D P, Takei H, Kwon Sym Wood D, et al. The cytotoxic effect of titanium particles phagocytosed by osteoblasts. J Biomed Mater Res, 1999, 46: 399–407

    Article  Google Scholar 

  38. Kwon S Y, Takei H, Pioletti D P, et al. Titanium particles inhibit osteoblast adhesion to fibronectin-coated substrates. J Orthop Res, 2000, 18: 203–211

    Article  Google Scholar 

  39. Martinez M E, Medina S, del Campo M T, et al. Effect of polyethylene particles on human osteoblastic cell growth. Biomaterials, 1998, 19: 183–187

    Article  Google Scholar 

  40. Allen M J, Myer B J, Millett P J, et al. The effects of particulate cobalt, chromium and cobalt-chromium alloy on human osteoblast-like cells in vitro. J Bone Joint Surg Br, 1997, 79: 475–482

    Article  Google Scholar 

  41. Jiang W, Kim B Y S, Rutka J T, et al. Nanoparticle-mediated cellular response is size-dependent. Nat Nanotechnol, 2008, 3: 145–150

    Article  Google Scholar 

  42. Gutwein L G, Webster T J. Increased viable osteoblast density in the presence of nanophase compared to conventional alumina and titania particles. Biomaterials, 2004, 25: 4175–4183

    Article  Google Scholar 

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Authors and Affiliations

  1. Chemical Biology Laboratory, College of Chemistry and Environmental Science, Hebei University, Baoding, 071002, China

    DanDan Liu & JinChao Zhang

  2. Key Laboratory of Biochip Research, Shenzhen Research Institute of City University of Hong Kong, Shenzhen, 518057, China

    DanDan Liu, ChangQing Yi & MengSu Yang

  3. Department of Biology and Chemistry, City University of Hong Kong, Hong Kong, China

    ChangQing Yi & MengSu Yang

Authors
  1. DanDan Liu
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  2. JinChao Zhang
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  3. ChangQing Yi
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  4. MengSu Yang
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Correspondence to JinChao Zhang or MengSu Yang.

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Liu, D., Zhang, J., Yi, C. et al. The effects of gold nanoparticles on the proliferation, differentiation, and mineralization function of MC3T3-E1 cells in vitro . Chin. Sci. Bull. 55, 1013–1019 (2010). https://doi.org/10.1007/s11434-010-0046-1

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  • DOI: https://doi.org/10.1007/s11434-010-0046-1

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