Chinese Science Bulletin

, Volume 54, Issue 17, pp 3035–3044 | Cite as

Regeneration and identification of interspecific asymmetric somatic hybrids obtained by donor-recipient fusion in cotton

  • LiLi Fu
  • XiYan Yang
  • XianLong Zhang
  • ZhiWei Wang
  • ChangHui Feng
  • ChuanXiang Liu
  • Pei-Yong Jiang
  • JinLong Zhang
Articles/Plant Cytology

Abstract

Asymmetric hybrids between Gossypium hirsutum (YZ-1) and G. davidsonii were obtained by donor-recipient fusion. YZ-1 was considered the recipient and was pretreated with iodoacetamide (IOA), while G. davidsonii was considered the donor and was irradiated with ultraviolet (UV) before fusion. YZ-1 protoplasts stopped growth when treated with 0.5 mmol/L IOA for 20 min, and G. davidsonii protoplasts stopped growth when irradiated with 38.7 J/cm2 UV for 30 s. Asymmetric somatic hybrids were obtained by electrofusion between the separately treated protoplasts of the 2 species. The regenerated plants were identified by morphological, cytological, and molecular analysis. Most regenerated plants derived from fused protoplasts displayed new morphology; some were intermediate between the two parents and a few displayed recipient-like morphology. Chromosome numbers in these somatic hybrids mostly ranged from 40 to 73. The hybridity was confirmed by random amplified polymorphic DNA and simple sequence repeat analysis. Organelle DNA inheritance of the YZ-1 and G. davidsonii somatic hybrid was investigated by cleaved amplified polymorphism sequence and chloroplast simple sequence repeat analysis, which indicated that recombination and rearrangements might have occurred in some regions of mitochondrial and chloroplastic DNA. This is the first report of completely asymmetric hybrid production via donor-recipient fusion between G. hirsutum and G. davidsonii, which is a novel case in hybrid production following the symmetric fusion and asymmetric fusion based on UV irradiation in cotton.

Keywords

cotton donor-recipient fusion IOA protoplast UV 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Sun Y Q, Zhang X L, Nie Y C, et al. Production and characterization of somatic hybrids between upland cotton (Gossypium hirsutum) and wild cotton (G. klotzschianum Anderss) via electrofusion. Theor Appl Genet, 2004, 109: 472–479CrossRefGoogle Scholar
  2. 2.
    Sun Y Q, Zhang X L, Nie Y C, et al. Production of fertile somatic hybrids of Gossypium hirsutum + G. bickii and G. hirsutum + G. stockii via protoplast fusion. Plant Cell Tiss Organ Cult, 2005, 83: 303–310CrossRefGoogle Scholar
  3. 3.
    Binsfeld P C, Schnabl H. Molecular and cytogenetic constitution of plants obtained via two different somatic hybridization methods. Plant Cell Rep, 2002, 21: 58–62CrossRefGoogle Scholar
  4. 4.
    Wardrop J, Fuller J, Powell W, et al. Exploiting plant somatic radiation hybrids for physical mapping of expressed sequence tags. Theor Appl Genet, 2004, 108: 343–348CrossRefGoogle Scholar
  5. 5.
    Sun Y Q, Nie Y C, Guo X P, et al. Somatic hybrids between Gossypium hirsutum L. (4×) and G. davidsonii Kellog (2×) produced by protoplast fusion. Euphytica, 2006, 151: 393–400CrossRefGoogle Scholar
  6. 6.
    Yang X Y, Zhang X L, Jin S X, et al. Production and characterization of asymmetric hybrids between upland cotton Coker 201 (Gossypium hirsutum) and wild cotton (G. klozschianum Anderss). Plant Cell Tiss Organ Cult, 2007, 89: 225–235CrossRefGoogle Scholar
  7. 7.
    Wang M Q, Zhao J S, Peng Z Y, et al. Chromosomes are eliminated in the symmetric fusion between Arabidopsis thaliana L. and Bupleurum scorzonerifolium Willd. Plant Cell Tiss Organ Cult, 2008, 92: 121–130CrossRefGoogle Scholar
  8. 8.
    Liu J H, Deng X X. Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis. Plant Cell Tiss Organ Cult, 1999, 59: 81–87CrossRefGoogle Scholar
  9. 9.
    Zhu Y S, Chen B T, Yu S W, et al. Transfer of bacterial blight resistance from Oryza meyeriana to O. sativa L. by asymmetric somatic hybridization. Chinese Sci Bull, 2004, 49: 1824–1824CrossRefGoogle Scholar
  10. 10.
    Hansen L N, Earle E D. Transfer of resistance to Xanthomonas campestris pv campestris into Brassica oleracea L. by protoplast. Theor Appl Genet, 1995, 91: 1293–1300Google Scholar
  11. 11.
    Varotto S, Nenz E, Lucchin M, et al. Production of asymmetric somatic hybrid plants between Cichorium intybus L. and Helianthus annuus L. Theor Appl Genet, 2001, 102: 950–956CrossRefGoogle Scholar
  12. 12.
    Gleba Y Y, Hinnisdaels S, Sidorov V A, et al. Intergeneric asymmetric hybrids between Nicotiana plumbaginifolia and Atropa belladonna obtained by “gamma-fusion”. Theor Appl Genet, 1988, 76: 760–766CrossRefGoogle Scholar
  13. 13.
    Hinnisdaels S, Bariller L, Mouras A, et al. Highly asymmetric intergeneric nuclear hybrids between Nicotiana and Petunia: evidence for recombinogenic and translocation events in somatic hybrid plants after “gamma”-fusion. Theor Appl Genet, 1991, 82: 609–614CrossRefGoogle Scholar
  14. 14.
    McCabe P E, Dunbar L J, Guri A, et al. T-DNA-tagged chromosome 12 in donor Lycopersicon esculentum × L. pennellii is retained in asymmetric somatic hybrids with recipient Solanum lycopersicoides. Theor Appl Genet, 1993, 86: 377–382CrossRefGoogle Scholar
  15. 15.
    Hall R D, Rouwendal G J A, Krens F A. Asymmetric somatic cell hybridization in plants. I. The early effects of (sub) lethal doses of UV and gamma radiation on the cell physiology and DNA integrity of cultured sugarbeet (Beta vulgaris L.) protoplasts. Mol Gen Genet, 1992, 234: 306–314Google Scholar
  16. 16.
    Hall R D, Rouwendal G J A, Krens F A. Asymmetric somatic cell hybridization in plants. II. Electrophoretic analysis of radiation-induced DNA damage and repair following the exposure of sugarbeet (Beta vulgaris L.) protoplasts to UV and gamma rays. Mol Gen Genet, 1992, 234: 315–324Google Scholar
  17. 17.
    Xia G M, Li Z Y, Wang S L, et al. Asymmetric somatic hybridization between haploid common wheat and UV-irradiated Haynaldia vilosa. Plant Sci, 1998, 137: 217–223CrossRefGoogle Scholar
  18. 18.
    Cabasson C M, Luro F, Ollitrault P, et al. Non-random inheritance of mitochondrial genomes in Citrus hybrids produced by protoplast fusion. Plant Cell Rep, 2001, 20: 604–609Google Scholar
  19. 19.
    Fu C H, Guo W W, Liu J H, et al. Regeneration of Citrus sinensis(+) clausena lansium intergeneric triploid and tetraploid somatic hybrids and their identification by molecular markers. In Vitro Cell Dev Biol Plant, 2003, 39: 360–364CrossRefGoogle Scholar
  20. 20.
    Cheng Y J, Guo W W, Deng X X. Molecular characterization of cytoplasmic and nuclear genomes in phenotypically abnormal Valencia orange (Citrus sinensis) + Meiwa kumquat (Fortunella crassifolia) intergeneric somatic hybrids. Plant Cell Rep, 2003, 21: 445–451Google Scholar
  21. 21.
    Cheng Y J, Guo W W, Deng X X. cpSSR: a new tool to analyze chloroplast genome of citrus somatic hybrids. Aca Bot Sin, 2003, 45(8): 906–909Google Scholar
  22. 22.
    Cai X D, Fu J, Deng X X, et al. Production and molecular characterization of potential seedless cybrid plants between pollen sterile Satsuma mandarin and two seedy Citrus cultivars. Plant Cell Tiss Organ Cult, 2007, 90: 275–283CrossRefGoogle Scholar
  23. 23.
    Kao K N, Michayluk M R. Nutrient requirements for growth of Vicia hajastana cells and protoplasts at a very low population density in liquid media. Planta, 1975, 126: 105–110CrossRefGoogle Scholar
  24. 24.
    Paterson A H, Brubaker C L, Wendel J F. A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP and PCR analysis. Plant Mol Biol Rep, 1993, 11: 122–127CrossRefGoogle Scholar
  25. 25.
    Lin Z, He D, Zhang X, et al. Linkage map construction and mapping QTL for cotton fiber quality using SRAP, SSR and RAPD. Plant Breeding, 2005, 124: 180–187CrossRefGoogle Scholar
  26. 26.
    Wills D M, Burke J M. Chloroplast DNA variation confirms a single origin of domesticated sunflower (Helianthus annuus L.). J Hered, 2006, 97: 403–408CrossRefGoogle Scholar
  27. 27.
    Jin S X, Liang S G, Zhang X L, et al. An efficient grafting system for transgenic plant recovery in cotton (Gossypium hirsutum L.). Plant Cell Tiss Organ Cult, 2006, 85: 181–185CrossRefGoogle Scholar
  28. 28.
    Zhao Z G, Hu T T, Ge X H, et al. Production and characterization of intergeneric somatic hybrids between Brassica napus and Orychophragmus violaceus and their backcrossing progenies. Plant Cell Rep, 2008, 27: 1611–1621CrossRefGoogle Scholar
  29. 29.
    Terada R, Yamashita Y, Nishibayashi S, et al. Somatic hybrids between Brassica oleracea and B. campestris: selection by the use of iodoacetamide inactivation and regeneration ability. Theor Appl Genet, 1987, 73: 379–384CrossRefGoogle Scholar
  30. 30.
    Forsberg J, Lagercrantz U, Glimelius K. Comparison of UV light, X-ray and restriction enzyme treatments as tools in production of asymmetric somatic hybrids between Brassica napus and Arabidopsis thaliana. Theor Appl Genet, 1998, 96: 1178–1185CrossRefGoogle Scholar
  31. 31.
    Vlahova M, Hinnisdaels S, Frulleux F, et al. UV irradiation as a tool for obtaining asymmetric somatic hybrids between Nicotiana plumbaginifolia and Lycopersicon esculetum. Theor Appl Genet, 1997, 94: 184–191CrossRefGoogle Scholar
  32. 32.
    Liu J H, Xu X Y, Deng X X. Intergeneric somatic hybridization and its application to crop genetic improvement. Plant Cell Tiss Organ Cult, 2005, 82: 19–44CrossRefGoogle Scholar
  33. 33.
    Guo W W, Prasad D, Cheng Y J, et al. Targeted cybridization in citrus: transfer of Satsuma cytoplasm to seedy cultivars for potential seedlessness. Plant Cell Rep, 2004, 22: 752–758CrossRefGoogle Scholar

Copyright information

© Science in China Press and Springer-Verlag GmbH 2009

Authors and Affiliations

  • LiLi Fu
    • 1
  • XiYan Yang
    • 1
  • XianLong Zhang
    • 1
  • ZhiWei Wang
    • 1
  • ChangHui Feng
    • 1
  • ChuanXiang Liu
    • 1
  • Pei-Yong Jiang
    • 1
  • JinLong Zhang
    • 1
  1. 1.National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhanChina

Personalised recommendations