Abstract
The outbreak of a novel influenza A (H1N1) virus across the globe poses a threat to human health. It is of paramount importance to develop a rapid, reliable and inexpensive diagnostic procedure. Based on the bioinformatic information from public database, primers specific for influenza A virus surface protein haemagglutinin (HA) of several subtypes (including H1, H2, H3, H5, H7 and H9) were designed. Primer-specific PCR products were subjected to sequencing for accurately distinguishing H1 and H3 subtypes from others. This sequencing-based detection method will not only be applied to rapid detection and simultaneous subtype identification of new influenza A virus H1N1, but also provide the strategies to monitor other new types of influenza virus with explosive potential.
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Yan, A., Ding, G., Zhou, Z. et al. Subtyping of type A influenza by sequencing the variable regions of HA gene specifically amplified with RT-PCR. Chin. Sci. Bull. 54, 2164–2167 (2009). https://doi.org/10.1007/s11434-009-0433-7
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DOI: https://doi.org/10.1007/s11434-009-0433-7