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Development and application of tumor-targeting magnetic nanoparticles FA-StNP@Fe2O3 for hyperthermia

  • Articles/Material Chemistry
  • Published:
Chinese Science Bulletin

Abstract

Modified magnetic starch nanoparticles (FA-StNP@Fe2O3) were synthesized by conjugating folic acid (FA-PEG-NH2) onto the surface of magnetic starch nanoparticles (StNP@Fe2O3) prepared by reverse microemulsion method. The synthesized FA-StNP@Fe2O3 was investigated by transmission electron microscopy and zeta potential analysis. The average size of its well dispersed particles was 250 nm. The iron concentration of 2 mg/g was detected by phenanthroline method. Placing FA-StNP@Fe2O3 nanoparticles in the alternating magnetic field for 30 min resulted in an increase in the suspension temperature from ambient temperature (37°C) to a value between 42°C and 43°C. Co-cultured nanoparticles and Hela cell line or normal HUEC-12 cell line, and the biological effects at the cellular level were investigated in the alternating magnetic field using MTT assay, Hochest-PI double staining and flow cytometry analysis. Experimental results showed that FA-StNP@Fe2O3 within acertain concentration range has no obvious effect on cell proliferation. When treated in the magnetic field, apoptosis rate on Hela induced by FA-StNP@Fe2O3 was 13.4%. Prussian blue staining analysis confirmed that the nanoparticles modified with folic acid had improved ability in tumor cell-targeting, and therefore, potential applications in biomedical and magnetocaloric areas. It is expected be applied in tumor targeting therapy in the near future.

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Authors and Affiliations

  1. State Key Laboratory of Chemo/Biosening and Chemometrics, Hunan University, Changsha, 410082, China

    YuanQing Zheng, ChunYi Tong, Bei Wang, HongDong Liao, Dan Li & XuanMing Liu

  2. Research Center of Biological Energy Sources and Biomaterial, Hunan University, Changsha, 410082, China

    YuanQing Zheng, ChunYi Tong, Bei Wang, HongDong Liao, Dan Li & XuanMing Liu

  3. Institute of Life Science and Biotechnology, Hunan University, Changsha, 410082, China

    YuanQing Zheng, ChunYi Tong, Bei Wang, Ying Xie, HongDong Liao, Dan Li & XuanMing Liu

Authors
  1. YuanQing Zheng
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  2. ChunYi Tong
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  3. Bei Wang
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  4. Ying Xie
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  5. HongDong Liao
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  6. Dan Li
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  7. XuanMing Liu
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Corresponding authors

Correspondence to Dan Li or XuanMing Liu.

Additional information

Supported by the Key Program for Science and Technology of Hunan Province (Grant No. 03NKY1001) and Key Construction Program of the National “985” Project.

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Zheng, Y., Tong, C., Wang, B. et al. Development and application of tumor-targeting magnetic nanoparticles FA-StNP@Fe2O3 for hyperthermia. Chin. Sci. Bull. 54, 2998–3004 (2009). https://doi.org/10.1007/s11434-009-0422-x

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  • DOI: https://doi.org/10.1007/s11434-009-0422-x

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